Invitrogen TUBA4A antibody

alpha Tubulin Monoclonal Antibody (DM1A)
Invitrogen
catalog: 62204

mouse monoclonal (DM1A)
reactivity: freshwater planarian, hamsters, Leishmania infantum, African green monkey, guinea pig, human, mouse, rat, dogs, chicken, bovine, pigs , fruit fly , domestic sheep, Xenopus laevis
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, other
citations: 206

quantity: 40 uL
price: US 277.00
to the supplier

alpha Tubulin Monoclonal Antibody (YL1/2)
Invitrogen
catalog: MA1-80017

rat monoclonal (YL1/2)
reactivity: Dictyostelium discoideum, African green monkey, Saccharomyces cerevisiae S288C, human, mouse, rat, dogs, pigs , fruit fly , Xenopus laevis, domestic rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 85

quantity: 500 ug
price: US 524.00
to the supplier

alpha Tubulin Monoclonal Antibody (B-5-1-2)
Invitrogen
catalog: 32-2500

mouse monoclonal (B-5-1-2)
reactivity: human, mouse, rat, dogs, brewer's yeast, thale cress, domestic rabbit
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
citations: 92

quantity: 100 ug
price: US 469.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eBioscience
Invitrogen
catalog: 14-4502-80

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 37

quantity: 25 ug
price: US 134.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eBioscience
Invitrogen
catalog: 14-4502-82

quantity: 100 ug
price: US 356.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), Alexa Fluor 488, eBioscience
Invitrogen
catalog: 53-4502-80

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: AF488
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 21

quantity: 25 ug
price: US 177.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), Alexa Fluor 488, eBioscience
Invitrogen
catalog: 53-4502-82

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: AF488
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 22

quantity: 100 ug
price: US 486.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eFluor 615, eBioscience
Invitrogen
catalog: 42-4502-82

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: eFluor 615
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 16

quantity: 100 ug
price: US 496.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eBioscience
Invitrogen
catalog: 14-4502-95

quantity: 10 mg
price: US 3685.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), eBioscience
Invitrogen
catalog: 14-4502-37

quantity: 2 mg
price: US 1482.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), Biotin, eBioscience
Invitrogen
catalog: 13-4502-80

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: biotin
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 15

quantity: 25 ug
price: US 166.00
to the supplier

alpha Tubulin Monoclonal Antibody (DM1A), Biotin, eBioscience
Invitrogen
catalog: 13-4502-82

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dogs, pigs
conjugate: biotin
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 15

quantity: 100 ug
price: US 399.00
to the supplier

alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-19489

domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunocytochemistry, flow cytometry
citations: 11

Immunoprecipitation of acetylated alpha-Tubulin was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an alpha-Tubulin polyclonal antibody (Product # PA5-19489) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).

Western blot analysis of HeLa Whole Cell Lysate using PA5-19489, alpha Tubulin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19489 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19489 staining of A431 Whole Cell Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Immunofluorescent staining of HeLa cells using PA5-19489, anti-Alpha Tubulin antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
quantity: 50 ug
price: US 478.00
to the supplier

alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA1-38814

domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 3

quantity: 1 mL
price: US 502.00
to the supplier