ERK1/ERK2 Monoclonal Antibody (ERK-7D8)
Invitrogen
catalog: 13-6200
mouse monoclonal (ERK-7D8)
reactivity: human, mouse, rat, dogs, Xenopus laevis
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 52
quantity: 200 ug
price: US 471.00
to the supplier
ERK1/ERK2 Monoclonal Antibody (K.913.4)
Invitrogen
catalog: MA5-15134
domestic rabbit monoclonal (K.913.4)
reactivity: Chinese hamsters, human, mouse, rat, dogs, bovine, pigs , zebrafish , fruit fly , roundworm
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 9
quantity: 200 uL
price: US 524.00
to the supplier
Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10 ...
Invitrogen
catalog: 700012
domestic rabbit recombinant (15H10L7)
reactivity: human, mouse, rat, dogs
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 14
quantity: 100 ug
price: US 552.00
to the supplier
ERK2 Monoclonal Antibody (6F8)
Invitrogen
catalog: MA1-099
mouse monoclonal (6F8)
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MAPK1 was performed by loading 25µg of A431 and K562 whole cell lysate and 10µg of HEK293T overexpression lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing MAPK1 (Product #MA1-099) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #32430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075).

Immunofluorescent analysis of MAPK1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing MAPK1 (Product# MA1-099), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification.
quantity: 100 ug
price: US 414.00
to the supplier
ERK2 Monoclonal Antibody (C1)
Invitrogen
catalog: MA5-50855
mouse monoclonal (C1)
reactivity: guinea pig, domestic goat, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 200 uL
price: US 454.00
to the supplier
ERK2 Monoclonal Antibody (C1)
Invitrogen
catalog: MA5-50856
mouse monoclonal (C1)
reactivity: domestic goat, guinea pig, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 20 uL
price: US 181.00
to the supplier