domestic rabbit polyclonal
reactivity: African green monkey, human, mouse, rat, dogs, domestic rabbit
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 45
reactivity: African green monkey, human, mouse, rat, dogs, domestic rabbit
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 45
Immunofluorescent analysis of Phalloidin (orange) and Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
Immunofluorescent analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS 0.1% triton-X (Product #37525) for 30 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Calreticulin (Product # PA3-900) at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product #35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554-Phalloidin (Product# 21834) at a dilution of 1:300 (i.e. 1unit/mL final concentration) in PBS and incubated for 30 min including. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249) at a dilution of 1µg/mL. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
Immunofluorescent analysis of Phalloidin (green) and Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (Product # 35562) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
quantity: 100 µL
price: US 399.00
to the supplier
chicken polyclonal
reactivity: Chinese hamsters, human, mouse, rat, dogs
application: western blot, immunocytochemistry
citations: 5
reactivity: Chinese hamsters, human, mouse, rat, dogs
application: western blot, immunocytochemistry
citations: 5
chicken polyclonal
reactivity: Chinese hamsters, human, mouse, rat, dogs, domestic rabbit
application: western blot, immunocytochemistry, immunoprecipitation
citations: 2
reactivity: Chinese hamsters, human, mouse, rat, dogs, domestic rabbit
application: western blot, immunocytochemistry, immunoprecipitation
citations: 2
domestic sheep polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunocytochemistry
reactivity: human, mouse, rat, dogs
application: western blot, immunocytochemistry