product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Gata-3 Monoclonal Antibody (TWAJ), PerCP-eFluor™ 710, eBioscience
catalog :
46-9966-41
quantity :
25 Tests
price :
US 170.00
clonality :
monoclonal
host :
rat
conjugate :
Percp/efluor 710
clone name :
TWAJ
reactivity :
human, mouse, pigs , rhesus macaque
application :
flow cytometry
more info or order :
citations: 61
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...
Levine A, Mendoza A, Hemmers S, Moltedo B, Niec R, Schizas M, et al. Stability and function of regulatory T cells expressing the transcription factor T-bet. Nature. 2017;546:421-425 pubmed publisher
  • flow cytometry; human; fig s3c
  • flow cytometry; mouse; loading ...; fig 4c
Lim A, Li Y, Lopez Lastra S, Stadhouders R, Paul F, Casrouge A, et al. Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation. Cell. 2017;168:1086-1100.e10 pubmed publisher
Verma M, Verma D, Sripada A, Sirohi K, Varma R, Sahu A, et al. NFκB1 inhibits memory formation and supports effector function of ILC2s in memory-driven asthma. Front Immunol. 2023;14:1217776 pubmed publisher
Korniotis S, Saichi M, Trichot C, Hoffmann C, Amblard E, Viguier A, et al. GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner. J Cell Sci. 2022;135: pubmed publisher
Antunes K, Cass xe3 o G, Santos L, Borges S, Poppe J, Gon xe7 alves J, et al. Airway Administration of Bacterial Lysate OM-85 Protects Mice Against Respiratory Syncytial Virus Infection. Front Immunol. 2022;13:867022 pubmed publisher
Leonardi I, Gao I, Lin W, Allen M, Li X, Fiers W, et al. Mucosal fungi promote gut barrier function and social behavior via Type 17 immunity. Cell. 2022;185:831-846.e14 pubmed publisher
Wilfahrt D, Philips R, Lama J, Kizerwetter M, Shapiro M, McCue S, et al. Histone deacetylase 3 represses cholesterol efflux during CD4+ T-cell activation. elife. 2021;10: pubmed publisher
Kalim K, Yang J, Modur V, Nguyen P, Li Y, Zheng Y, et al. Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity. Front Immunol. 2021;12:726393 pubmed publisher
Chao J, Korzinkin M, Zhavoronkov A, Ozerov I, Walker M, Higgins K, et al. Effector T cell responses unleashed by regulatory T cell ablation exacerbate oral squamous cell carcinoma. Cell Rep Med. 2021;2:100399 pubmed publisher
Verma M, Michalec L, Sripada A, McKay J, Sirohi K, Verma D, et al. The molecular and epigenetic mechanisms of innate lymphoid cell (ILC) memory and its relevance for asthma. J Exp Med. 2021;218: pubmed publisher
Zutshi S, Sarode A, Ghosh S, Jha M, Sudan R, Kumar S, et al. LmjF.36.3850, a novel hypothetical Leishmania major protein, contributes to the infection. Immunology. 2021;163:460-477 pubmed publisher
Turner J, Stephen Victor E, Wang S, Rivas M, Abdel Gadir A, Harb H, et al. Regulatory T Cell-Derived TGF-β1 Controls Multiple Checkpoints Governing Allergy and Autoimmunity. Immunity. 2020;53:1202-1214.e6 pubmed publisher
Stas M, Koch M, Stadler M, Sawyer S, Sassu E, Mair K, et al. NK and T Cell Differentiation at the Maternal-Fetal Interface in Sows During Late Gestation. Front Immunol. 2020;11:582065 pubmed publisher
Liu G, Deng X, Li X, Cao Y, Xing Y, Zhou P, et al. Expansion of Group 2 Innate Lymphoid Cells in Patients with End-Stage Renal Disease and Their Clinical Significance. J Immunol. 2020;205:36-44 pubmed publisher
Zhao X, Zhou L, Chen Z, Ji Y, Peng X, Qi L, et al. The obesity-induced adipokine sST2 exacerbates adipose Treg and ILC2 depletion and promotes insulin resistance. Sci Adv. 2020;6:eaay6191 pubmed publisher
Stokes J, Hoffman E, Molina M, Kummet N, Simpson R, Zeng Y, et al. Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia. Oncoimmunology. 2020;9:1758011 pubmed publisher
Sidwell T, Liao Y, Garnham A, Vasanthakumar A, Gloury R, Blume J, et al. Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis. Nat Commun. 2020;11:252 pubmed publisher
Eissmann M, Dijkstra C, Jarnicki A, Phesse T, Brunnberg J, Poh A, et al. IL-33-mediated mast cell activation promotes gastric cancer through macrophage mobilization. Nat Commun. 2019;10:2735 pubmed publisher
Esterházy D, Canesso M, Mesin L, Muller P, de Castro T, Lockhart A, et al. Compartmentalized gut lymph node drainage dictates adaptive immune responses. Nature. 2019;569:126-130 pubmed publisher
Xu W, Cherrier D, Chea S, Vosshenrich C, Serafini N, Petit M, et al. An Id2RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials. Immunity. 2019;50:1054-1068.e3 pubmed publisher
Grizotte Lake M, Zhong G, Duncan K, Kirkwood J, Iyer N, Smolenski I, et al. Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis. Immunity. 2018;49:1103-1115.e6 pubmed publisher
Dutton E, Camelo A, Sleeman M, Herbst R, Carlesso G, Belz G, et al. Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity. Wellcome Open Res. 2017;2:117 pubmed publisher
Liang P, Peng S, Zhang M, Ma Y, Zhen X, Li H. Huai Qi Huang corrects the balance of Th1/Th2 and Treg/Th17 in an ovalbumin-induced asthma mouse model. Biosci Rep. 2017;37: pubmed publisher
Elderman M, van Beek A, Brandsma E, de Haan B, Savelkoul H, De Vos P, et al. Sex impacts Th1 cells, Tregs, and DCs in both intestinal and systemic immunity in a mouse strain and location-dependent manner. Biol Sex Differ. 2016;7:21 pubmed publisher
Monticelli L, Buck M, Flamar A, Saenz S, Tait Wojno E, Yudanin N, et al. Arginase 1 is an innate lymphoid-cell-intrinsic metabolic checkpoint controlling type 2 inflammation. Nat Immunol. 2016;17:656-65 pubmed publisher
Deng Y, Yang Y, Wang S, Li F, Liu M, Hua Q, et al. Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced Inflammation by Targeting GATA-3. PLoS ONE. 2015;10:e0139322 pubmed publisher
Drummond R, Dambuza I, Vautier S, Taylor J, Reid D, Bain C, et al. CD4(+) T-cell survival in the GI tract requires dectin-1 during fungal infection. Mucosal Immunol. 2016;9:492-502 pubmed publisher
Li L, Yang C, Zhao Z, Xu B, Zheng M, Zhang C, et al. Skewed T-helper (Th)1/2- and Th17/T regulatory‑cell balances in patients with renal cell carcinoma. Mol Med Rep. 2015;11:947-53 pubmed publisher
Ahlfors H, Morrison P, Duarte J, Li Y, Biro J, Tolaini M, et al. IL-22 fate reporter reveals origin and control of IL-22 production in homeostasis and infection. J Immunol. 2014;193:4602-13 pubmed publisher
Commandeur S, Coppola M, Dijkman K, Friggen A, van Meijgaarden K, van den Eeden S, et al. Clonal analysis of the T-cell response to in vivo expressed Mycobacterium tuberculosis protein Rv2034, using a CD154 expression based T-cell cloning method. PLoS ONE. 2014;9:e99203 pubmed publisher
Lin C, Bradstreet T, Schwarzkopf E, Sim J, Carrero J, Chou C, et al. Bhlhe40 controls cytokine production by T cells and is essential for pathogenicity in autoimmune neuroinflammation. Nat Commun. 2014;5:3551 pubmed publisher
Uchiyama M, Jin X, Matsuda H, Bashuda H, Imazuru T, Shimokawa T, et al. An agonistic anti-BTLA mAb (3C10) induced generation of IL-10-dependent regulatory CD4+ T cells and prolongation of murine cardiac allograft. Transplantation. 2014;97:301-9 pubmed publisher
Villegas Mendez A, Gwyer Findlay E, de Souza J, Grady L, Saris C, Lane T, et al. WSX-1 signalling inhibits CD4? T cell migration to the liver during malaria infection by repressing chemokine-independent pathways. PLoS ONE. 2013;8:e78486 pubmed publisher
Hu T, Wang H, Simmons A, Bajana S, Zhao Y, Kovats S, et al. Increased level of E protein activity during invariant NKT development promotes differentiation of invariant NKT2 and invariant NKT17 subsets. J Immunol. 2013;191:5065-73 pubmed publisher
KEMP K, Lin Z, Zhao F, Gao B, Song J, Zhang K, et al. The serine-threonine kinase inositol-requiring enzyme 1? (IRE1?) promotes IL-4 production in T helper cells. J Biol Chem. 2013;288:33272-82 pubmed publisher
Mielke L, Groom J, Rankin L, Seillet C, Masson F, Putoczki T, et al. TCF-1 controls ILC2 and NKp46+ROR?t+ innate lymphocyte differentiation and protection in intestinal inflammation. J Immunol. 2013;191:4383-91 pubmed publisher
Blom L, Poulsen L. In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells. J Immunol Methods. 2013;397:55-60 pubmed publisher
Durant L, Makris S, Voorburg C, Loebbermann J, Johansson C, Openshaw P. Regulatory T cells prevent Th2 immune responses and pulmonary eosinophilia during respiratory syncytial virus infection in mice. J Virol. 2013;87:10946-54 pubmed publisher
Gwyer Findlay E, Villegas Mendez A, de Souza J, Inkson C, Shaw T, Saris C, et al. IL-27 receptor signaling regulates CD4+ T cell chemotactic responses during infection. J Immunol. 2013;190:4553-61 pubmed publisher
Kleinewietfeld M, Manzel A, Titze J, Kvakan H, Yosef N, Linker R, et al. Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells. Nature. 2013;496:518-22 pubmed publisher
Peters J, Tjabringa G, Fasse E, de Oliveira V, Schalkwijk J, Koenen H, et al. Co-culture of healthy human keratinocytes and T-cells promotes keratinocyte chemokine production and ROR?t-positive IL-17 producing T-cell populations. J Dermatol Sci. 2013;69:44-53 pubmed publisher
Chen J, Huang P, Lee C, Chen P, Chen H. A bovine whey protein extract can induce the generation of regulatory T cells and shows potential to alleviate asthma symptoms in a murine asthma model. Br J Nutr. 2013;109:1813-20 pubmed publisher
Sawant D, Sehra S, Nguyen E, Jadhav R, Englert K, Shinnakasu R, et al. Bcl6 controls the Th2 inflammatory activity of regulatory T cells by repressing Gata3 function. J Immunol. 2012;189:4759-69 pubmed publisher
Blom L, Poulsen L. IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures. J Immunol. 2012;189:4331-7 pubmed publisher
Gibson H, Mishra A, Chan D, Hake T, Porcu P, Wong H. Impaired proteasome function activates GATA3 in T cells and upregulates CTLA-4: relevance for Sézary syndrome. J Invest Dermatol. 2013;133:249-57 pubmed publisher
Shatynski K, Chen H, Kwon J, Williams M. Decreased STAT5 phosphorylation and GATA-3 expression in NOX2-deficient T cells: role in T helper development. Eur J Immunol. 2012;42:3202-11 pubmed publisher
Centuori S, Trad M, LaCasse C, Alizadeh D, Larmonier C, Hanke N, et al. Myeloid-derived suppressor cells from tumor-bearing mice impair TGF-?-induced differentiation of CD4+CD25+FoxP3+ Tregs from CD4+CD25-FoxP3- T cells. J Leukoc Biol. 2012;92:987-97 pubmed publisher
Grundström J, Linton L, Thunberg S, Forsslund H, Janczewska I, Befrits R, et al. Altered immunoregulatory profile during anti-tumour necrosis factor treatment of patients with inflammatory bowel disease. Clin Exp Immunol. 2012;169:137-47 pubmed publisher
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Radulovic K, Manta C, Rossini V, Holzmann K, Kestler H, Wegenka U, et al. CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential. J Immunol. 2012;188:2001-13 pubmed publisher
Hansmann L, Schmidl C, Kett J, Steger L, Andreesen R, Hoffmann P, et al. Dominant Th2 differentiation of human regulatory T cells upon loss of FOXP3 expression. J Immunol. 2012;188:1275-82 pubmed publisher
LaCasse C, Janikashvili N, Larmonier C, Alizadeh D, Hanke N, Kartchner J, et al. Th-1 lymphocytes induce dendritic cell tumor killing activity by an IFN-?-dependent mechanism. J Immunol. 2011;187:6310-7 pubmed publisher
Nakahira M, Nakanishi K. Requirement of GATA-binding protein 3 for II13 gene expression in IL-18-stimulated Th1 cells. Int Immunol. 2011;23:761-72 pubmed publisher
Blom L, Poulsen B, Jensen B, Hansen A, Poulsen L. IL-33 induces IL-9 production in human CD4+ T cells and basophils. PLoS ONE. 2011;6:e21695 pubmed publisher
Hebenstreit D, Fang M, Gu M, Charoensawan V, van Oudenaarden A, Teichmann S. RNA sequencing reveals two major classes of gene expression levels in metazoan cells. Mol Syst Biol. 2011;7:497 pubmed publisher
Lu Y, Malmhäll C, Sjöstrand M, Radinger M, O Neil S, Lotvall J, et al. Expansion of CD4(+) CD25(+) and CD25(-) T-Bet, GATA-3, Foxp3 and ROR?t cells in allergic inflammation, local lung distribution and chemokine gene expression. PLoS ONE. 2011;6:e19889 pubmed publisher
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KEMP K, Levin S, Bryce P, Stein P. Lck mediates Th2 differentiation through effects on T-bet and GATA-3. J Immunol. 2010;184:4178-84 pubmed publisher
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Winer S, Chan Y, Paltser G, Truong D, Tsui H, Bahrami J, et al. Normalization of obesity-associated insulin resistance through immunotherapy. Nat Med. 2009;15:921-9 pubmed publisher
Guan H, Nagarkatti P, Nagarkatti M. Role of CD44 in the differentiation of Th1 and Th2 cells: CD44-deficiency enhances the development of Th2 effectors in response to sheep RBC and chicken ovalbumin. J Immunol. 2009;183:172-80 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Gata-3 Monoclonal Antibody (TWAJ), PerCP-eFluor™ 710, eBioscience
Catalog # :
46-9966-41
Quantity :
25 Tests
Price :
US 170.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Human, Mouse, Porcine, Rhesus Monkey
Applications :
Flow Cytometry: 5 µL (0.06 µg)/test
Species :
Human, Mouse, Porcine, Rhesus Monkey
Clone :
TWAJ
Isotype :
IgG2b, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 5 µL (0.06 µg)/test
Aliases :
EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA