PDHA1 Monoclonal Antibody (9H9AF5) | Invitrogen 459400 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PDHA1 Monoclonal Antibody (9H9AF5)

catalog :

459400

quantity :

100 µg

price :

425 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9H9AF5

reactivity :

human, mouse, rat, bovine

application :

western blot, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4b	Park S, Safi R, Liu X, Baldi R, Liu W, Liu J, et al. Inhibition of ERRα Prevents Mitochondrial Pyruvate Uptake Exposing NADPH-Generating Pathways as Targetable Vulnerabilities in Breast Cancer. Cell Rep. 2019;27:3587-3601.e4 pubmed publisher
western blot; human; 1:500; loading ...; fig 6c	Santacatterina F, Sánchez Aragó M, Catalán García M, Garrabou G, de Arenas C, Grau J, et al. Pyruvate kinase M2 and the mitochondrial ATPase Inhibitory Factor 1 provide novel biomarkers of dermatomyositis: a metabolic link to oncogenesis. J Transl Med. 2017;15:29 pubmed publisher
western blot; mouse; loading ...; fig 4a	Takahashi Y, Tamura Y, Matsunaga Y, Kitaoka Y, Terada S, Hatta H. Effects of Taurine Administration on Carbohydrate Metabolism in Skeletal Muscle during the Post-Exercise Phase. J Nutr Sci Vitaminol (Tokyo). 2016;62:257-264 pubmed
western blot; mouse; loading ...	Goguet Rubio P, Seyran B, Gayte L, Bernex F, Sutter A, Delpech H, et al. E4F1-mediated control of pyruvate dehydrogenase activity is essential for skin homeostasis. Proc Natl Acad Sci U S A. 2016;113:11004-9 pubmed publisher
western blot; human; loading ...; fig 3e	Titov D, Cracan V, Goodman R, Peng J, Grabarek Z, Mootha V. Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio. Science. 2016;352:231-5 pubmed publisher
western blot; human; fig s2	Jiang L, Shestov A, Swain P, Yang C, Parker S, Wang Q, et al. Reductive carboxylation supports redox homeostasis during anchorage-independent growth. Nature. 2016;532:255-8 pubmed publisher
western blot; mouse	Kerr E, Gaude E, Turrell F, Frezza C, Martins C. Mutant Kras copy number defines metabolic reprogramming and therapeutic susceptibilities. Nature. 2016;531:110-3 pubmed publisher
western blot; human	Prabhu A, Sarcar B, Miller C, Kim S, Nakano I, Forsyth P, et al. Ras-mediated modulation of pyruvate dehydrogenase activity regulates mitochondrial reserve capacity and contributes to glioblastoma tumorigenesis. Neuro Oncol. 2015;17:1220-30 pubmed publisher
western blot; human; 1:500; fig 6	Madhok B, Yeluri S, Perry S, Hughes T, Jayne D. Dichloroacetate induces apoptosis and cell-cycle arrest in colorectal cancer cells. Br J Cancer. 2010;102:1746-52 pubmed publisher
	Flicker D, Sancak Y, Mick E, Goldberger O, Mootha V. Exploring the In Vivo Role of the Mitochondrial Calcium Uniporter in Brown Fat Bioenergetics. Cell Rep. 2019;27:1364-1375.e5 pubmed publisher
	Yao C, Wang R, Wang Y, Kung C, Weber J, Patti G. Mitochondrial fusion supports increased oxidative phosphorylation during cell proliferation. elife. 2019;8: pubmed publisher
	Miotto P, McGlory C, Holloway T, Phillips S, Holloway G. Sex differences in mitochondrial respiratory function in human skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2018;314:R909-R915 pubmed publisher
	Chen J, Guccini I, Di Mitri D, Brina D, Revandkar A, Sarti M, et al. Compartmentalized activities of the pyruvate dehydrogenase complex sustain lipogenesis in prostate cancer. Nat Genet. 2018;50:219-228 pubmed publisher
	Park S, Chang C, Safi R, Liu X, Baldi R, Jasper J, et al. ERR?-Regulated Lactate Metabolism Contributes to Resistance to Targeted Therapies in Breast Cancer. Cell Rep. 2016;15:323-35 pubmed publisher

product information

Product Type :

Antibody

Product Name :

PDHA1 Monoclonal Antibody (9H9AF5)

Catalog # :

459400

Quantity :

100 µg

Price :

425 USD

Clonality :

Monoclonal

Purity :

IgG fraction

Host :

Mouse

Reactivity :

Bovine, Human, Mouse, Rat

Applications :

Flow Cytometry: 1 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Western Blot: 1.0 µg/mL

Species :

Bovine, Human, Mouse, Rat

Clone :

9H9AF5

Isotype :

IgG1

Storage :

4° C, do not freeze

Description :

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen :

Human recombinant full-length PDH E1-alpha

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Western Blot: 1.0 µg/mL

Aliases :

PDHA; PDHA1; Pdha-1; PDHAD; PDHCE1A; PDHE1-A type I; PHE1A; pyruvate dehydrogenase; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase alpha 1; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 alpha 1 subunit; pyruvate dehydrogenase E1 component alpha subunit; pyruvate dehydrogenase E1 component alpha subunit precursor; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; pyruvate dehydrogenase E1 subunit; pyruvate dehydrogenase E1 subunit alpha 1; pyruvate dehydrogenase E1alpha subunit

more info or order :

Invitrogen product webpage