product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
NDUFB8 Monoclonal Antibody (20E9DH10C12)
catalog :
459210
quantity :
100 µg
price :
US 454
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
20E9DH10C12
reactivity :
human, mouse, rat, bovine, pigs
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 25
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig s2l
Basse A, Agerholm M, Farup J, Dalbram E, Nielsen J, Ørtenblad N, et al. Nampt controls skeletal muscle development by maintaining Ca2+ homeostasis and mitochondrial integrity. Mol Metab. 2021;53:101271 pubmed publisher
  • western blot; human; 1:5000; loading ...
Borgia D, Malena A, Spinazzi M, Desbats M, Salviati L, Russell A, et al. Increased mitophagy in the skeletal muscle of spinal and bulbar muscular atrophy patients. Hum Mol Genet. 2017;26:1087-1103 pubmed publisher
  • western blot; mouse; fig 1d
D Andrea A, Gritti I, Nicoli P, Giorgio M, Doni M, Conti A, et al. The mitochondrial translation machinery as a therapeutic target in Myc-driven lymphomas. Oncotarget. 2016;7:72415-72430 pubmed publisher
  • western blot; human; fig 5
Scott A, Wilkinson A, Wilkinson J. Basal metabolic state governs AIF-dependent growth support in pancreatic cancer cells. BMC Cancer. 2016;16:286 pubmed publisher
  • western blot; mouse
Brandauer J, Andersen M, Kellezi H, Risis S, Frøsig C, Vienberg S, et al. AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD. Front Physiol. 2015;6:85 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:2000
Braun M, Hettinger N, Koentges C, Pfeil K, Cimolai M, Hoffmann M, et al. Myocardial mitochondrial and contractile function are preserved in mice lacking adiponectin. PLoS ONE. 2015;10:e0119416 pubmed publisher
  • western blot; mouse
Zou P, Liu L, Zheng L, Liu L, Stoneman R, Cho A, et al. Targeting FoxO1 with AS1842856 suppresses adipogenesis. Cell Cycle. 2014;13:3759-67 pubmed publisher
  • western blot; rat; fig 9
Dun Y, Vargas J, Brot N, Finnemann S. Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells. Free Radic Biol Med. 2013;65:1340-1351 pubmed publisher
  • western blot; mouse; 1:2000; fig 1
  • western blot; human; fig 2
Morató L, Galino J, Ruiz M, Calingasan N, Starkov A, Dumont M, et al. Pioglitazone halts axonal degeneration in a mouse model of X-linked adrenoleukodystrophy. Brain. 2013;136:2432-43 pubmed publisher
  • western blot; human; fig 5
Lewis E, Wilkinson A, Jackson J, Mehra R, Varambally S, Chinnaiyan A, et al. The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells. J Biol Chem. 2012;287:43862-75 pubmed publisher
Sang L, He Y, Kang J, Ye H, Bai W, Luo X, et al. Mitochondrial Deoxyguanosine Kinase Regulates NAD+ Biogenesis Independent of Mitochondria Complex I Activity. Front Oncol. 2020;10:570656 pubmed publisher
Koh J, Kim K, Park S, Kim Y, Kim J. PPARδ Attenuates Alcohol-Mediated Insulin Resistance by Enhancing Fatty Acid-Induced Mitochondrial Uncoupling and Antioxidant Defense in Skeletal Muscle. Front Physiol. 2020;11:749 pubmed publisher
Timper K, Del Río Martín A, Cremer A, Bremser S, Alber J, Giavalisco P, et al. GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function. Cell Metab. 2020;31:1189-1205.e13 pubmed publisher
Acin Perez R, Benador I, Petcherski A, Veliova M, Benavides G, Lagarrigue S, et al. A novel approach to measure mitochondrial respiration in frozen biological samples. EMBO J. 2020;39:e104073 pubmed publisher
Dollerup O, Chubanava S, Agerholm M, Søndergård S, Altıntaş A, Møller A, et al. Nicotinamide riboside does not alter mitochondrial respiration, content or morphology in skeletal muscle from obese and insulin-resistant men. J Physiol. 2020;598:731-754 pubmed publisher
Lin S, Huang C, Sun J, Bollt O, Wang X, Martine E, et al. The mitochondrial deoxyguanosine kinase is required for cancer cell stemness in lung adenocarcinoma. EMBO Mol Med. 2019;11:e10849 pubmed publisher
Han J, Kim S, Ryu M, Jang Y, Lee M, Ju X, et al. Chloramphenicol Mitigates Oxidative Stress by Inhibiting Translation of Mitochondrial Complex I in Dopaminergic Neurons of Toxin-Induced Parkinson's Disease Model. Oxid Med Cell Longev. 2019;2019:4174803 pubmed publisher
Lin S, Huang C, Gunda V, Sun J, Chellappan S, Li Z, et al. Fascin Controls Metastatic Colonization and Mitochondrial Oxidative Phosphorylation by Remodeling Mitochondrial Actin Filaments. Cell Rep. 2019;28:2824-2836.e8 pubmed publisher
Polyzos A, Lee D, Datta R, Hauser M, Budworth H, Holt A, et al. Metabolic Reprogramming in Astrocytes Distinguishes Region-Specific Neuronal Susceptibility in Huntington Mice. Cell Metab. 2019;29:1258-1273.e11 pubmed publisher
Timper K, Paeger L, Sánchez Lasheras C, Varela L, Jais A, Nolte H, et al. Mild Impairment of Mitochondrial OXPHOS Promotes Fatty Acid Utilization in POMC Neurons and Improves Glucose Homeostasis in Obesity. Cell Rep. 2018;25:383-397.e10 pubmed publisher
Scott A, Walker S, Krank J, Wilkinson A, Johnson K, Lewis E, et al. AIF promotes a JNK1-mediated cadherin switch independently of respiratory chain stabilization. J Biol Chem. 2018;293:14707-14722 pubmed publisher
Agerholm M, Dall M, Jensen B, Prats C, Madsen S, Basse A, et al. Perturbations of NAD+ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle. Am J Physiol Endocrinol Metab. 2018;314:E377-E395 pubmed publisher
Zhang J, Gao Q, Zhou Y, Dier U, Hempel N, Hochwald S. Focal adhesion kinase-promoted tumor glucose metabolism is associated with a shift of mitochondrial respiration to glycolysis. Oncogene. 2016;35:1926-42 pubmed publisher
Morató L, Ruiz M, Boada J, Calingasan N, Galino J, Guilera C, et al. Activation of sirtuin 1 as therapy for the peroxisomal disease adrenoleukodystrophy. Cell Death Differ. 2015;22:1742-53 pubmed publisher
Lasagna Reeves C, Castillo Carranza D, Sengupta U, Clos A, Jackson G, Kayed R. Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice. Mol Neurodegener. 2011;6:39 pubmed publisher
product information
Product Type :
Antibody
Product Name :
NDUFB8 Monoclonal Antibody (20E9DH10C12)
Catalog # :
459210
Quantity :
100 µg
Price :
US 454
Clonality :
Monoclonal
Purity :
Ammonium sulfate precipitation
Host :
Mouse
Reactivity :
Bovine, Human, Mouse, Porcine, Rat
Applications :
Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): 1:100, Western Blot: 0.5 - 1 µg/mL
Species :
Bovine, Human, Mouse, Porcine, Rat
Clone :
20E9DH10C12
Isotype :
IgG1, kappa
Storage :
4° C
Description :
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt]
Immunogen :
Bovine Complex I
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): 1:100, Western Blot: 0.5 - 1 µg/mL
Aliases :
19 kDa; 2900010I05Rik; AI987932; ASHI; CI-ASHI; complex I ASHI subunit; complex I-ASHI; NADH dehydrogenase; NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; NADH dehydrogenase 1 beta subcomplex 8; NADH:ubiquinone oxidoreductase ASHI subunit; NADH:ubiquinone oxidoreductase subunit B8; NADH-ubiquinone oxidoreductase ASHI subunit; NDUFB8
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA