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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
NDUFA9 Monoclonal Antibody (20C11B11B11)
catalog :
459100
quantity :
100 µg
price :
US 459
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
20C11B11B11
reactivity :
human, mouse, rat, bovine
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 46
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig s3-1b
Cheng C, Wooten J, Gibbs Z, McGlynn K, Mishra P, Whitehurst A. Sperm-specific COX6B2 enhances oxidative phosphorylation, proliferation, and survival in human lung adenocarcinoma. elife. 2020;9: pubmed publisher
  • western blot; mouse; loading ...; fig 2b
Koh J, Hancock C, Terada S, Higashida K, Holloszy J, Han D. PPARβ Is Essential for Maintaining Normal Levels of PGC-1α and Mitochondria and for the Increase in Muscle Mitochondria Induced by Exercise. Cell Metab. 2017;25:1176-1185.e5 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2b
Ariyoshi M, Katane M, Hamase K, Miyoshi Y, Nakane M, Hoshino A, et al. D-Glutamate is metabolized in the heart mitochondria. Sci Rep. 2017;7:43911 pubmed publisher
  • western blot; mouse; loading ...; fig 6b
Schatton D, Pla Martín D, Marx M, Hansen H, Mourier A, Nemazanyy I, et al. CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J Cell Biol. 2017;216:675-693 pubmed publisher
  • western blot; human; loading ...; fig 2b
Cenini G, Rüb C, Bruderek M, Voos W. Amyloid ?-peptides interfere with mitochondrial preprotein import competence by a coaggregation process. Mol Biol Cell. 2016;27:3257-3272 pubmed
  • western blot; human; loading ...; fig 6a
Rohlenova K, Sachaphibulkij K, Stursa J, Bezawork Geleta A, Blecha J, Endaya B, et al. Selective Disruption of Respiratory Supercomplexes as a New Strategy to Suppress Her2high Breast Cancer. Antioxid Redox Signal. 2017;26:84-103 pubmed publisher
  • western blot; mouse; fig 6
Inoue T, Ikeda M, Ide T, Fujino T, Matsuo Y, Arai S, et al. Twinkle overexpression prevents cardiac rupture after myocardial infarction by alleviating impaired mitochondrial biogenesis. Am J Physiol Heart Circ Physiol. 2016;311:H509-19 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Seiferling D, Szczepanowska K, Becker C, Senft K, Hermans S, Maiti P, et al. Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt. EMBO Rep. 2016;17:953-64 pubmed publisher
  • western blot; human; fig 5
Scott A, Wilkinson A, Wilkinson J. Basal metabolic state governs AIF-dependent growth support in pancreatic cancer cells. BMC Cancer. 2016;16:286 pubmed publisher
  • western blot; mouse; fig s3a
Wai T, García Prieto J, Baker M, Merkwirth C, Benit P, Rustin P, et al. Imbalanced OPA1 processing and mitochondrial fragmentation cause heart failure in mice. Science. 2015;350:aad0116 pubmed publisher
  • western blot; mouse; fig 5
Korwitz A, Merkwirth C, Richter Dennerlein R, Tröder S, Sprenger H, Quirós P, et al. Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria. J Cell Biol. 2016;212:157-66 pubmed publisher
  • western blot; mouse
Ikeda M, Ide T, Fujino T, Arai S, Saku K, Kakino T, et al. Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress. PLoS ONE. 2015;10:e0119687 pubmed publisher
  • western blot; rat; 1:1000
Gomez A, Gomez J, Lopez Torres M, Naudi A, Mota Martorell N, Pamplona R, et al. Cysteine dietary supplementation reverses the decrease in mitochondrial ROS production at complex I induced by methionine restriction. J Bioenerg Biomembr. 2015;47:199-208 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Zheng H, Fu J, Xue P, Zhao R, Dong J, Liu D, et al. CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion. Antioxid Redox Signal. 2015;22:819-31 pubmed publisher
  • western blot; rat; 1:2000
Quan X, Nguyen T, Choi S, Xu S, Das R, Cha S, et al. Essential role of mitochondrial Ca2+ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells. J Biol Chem. 2015;290:4086-96 pubmed publisher
  • western blot; human; 1:1000
Ishikawa K, Saiki S, Furuya N, Yamada D, Imamichi Y, Li Y, et al. P150glued-associated disorders are caused by activation of intrinsic apoptotic pathway. PLoS ONE. 2014;9:e94645 pubmed publisher
  • western blot; mouse; 1:2000; fig s15
Hoshino A, Mita Y, Okawa Y, Ariyoshi M, Iwai Kanai E, Ueyama T, et al. Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart. Nat Commun. 2013;4:2308 pubmed publisher
  • western blot; mouse; fig 2
Kim S, Asaka M, Higashida K, Takahashi Y, Holloszy J, Han D. ?-Adrenergic stimulation does not activate p38 MAP kinase or induce PGC-1? in skeletal muscle. Am J Physiol Endocrinol Metab. 2013;304:E844-52 pubmed publisher
  • western blot; human; fig 5
Lewis E, Wilkinson A, Jackson J, Mehra R, Varambally S, Chinnaiyan A, et al. The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells. J Biol Chem. 2012;287:43862-75 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; fig 1
Lauritzen K, Cheng C, Wiksen H, Bergersen L, Klungland A. Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses. DNA Repair (Amst). 2011;10:639-53 pubmed publisher
  • western blot; human; 1:1000
Brosel S, Yang H, Tanji K, Bonilla E, Schon E. Unexpected vascular enrichment of SCO1 over SCO2 in mammalian tissues: implications for human mitochondrial disease. Am J Pathol. 2010;177:2541-8 pubmed publisher
Delavallée L, Mathiah N, Cabon L, Mazeraud A, Brunelle Navas M, Lerner L, et al. Mitochondrial AIF loss causes metabolic reprogramming, caspase-independent cell death blockade, embryonic lethality, and perinatal hydrocephalus. Mol Metab. 2020;40:101027 pubmed publisher
Timper K, Del Río Martín A, Cremer A, Bremser S, Alber J, Giavalisco P, et al. GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function. Cell Metab. 2020;31:1189-1205.e13 pubmed publisher
Wende A, Schell J, Ha C, Pepin M, Khalimonchuk O, Schwertz H, et al. Maintaining Myocardial Glucose Utilization in Diabetic Cardiomyopathy Accelerates Mitochondrial Dysfunction. Diabetes. 2020;69:2094-2111 pubmed publisher
Mota Martorell N, Jove M, Pradas I, Sanchez I, Gomez J, Naudi A, et al. Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity. Redox Biol. 2020;34:101539 pubmed publisher
Moschinger M, Hilse K, Rupprecht A, Zeitz U, Erben R, Rülicke T, et al. Age-related sex differences in the expression of important disease-linked mitochondrial proteins in mice. Biol Sex Differ. 2019;10:56 pubmed publisher
Hu X, Calton M, Tang S, Vollrath D. Depletion of Mitochondrial DNA in Differentiated Retinal Pigment Epithelial Cells. Sci Rep. 2019;9:15355 pubmed publisher
Lin S, Huang C, Sun J, Bollt O, Wang X, Martine E, et al. The mitochondrial deoxyguanosine kinase is required for cancer cell stemness in lung adenocarcinoma. EMBO Mol Med. 2019;11:e10849 pubmed publisher
Han J, Kim S, Ryu M, Jang Y, Lee M, Ju X, et al. Chloramphenicol Mitigates Oxidative Stress by Inhibiting Translation of Mitochondrial Complex I in Dopaminergic Neurons of Toxin-Induced Parkinson's Disease Model. Oxid Med Cell Longev. 2019;2019:4174803 pubmed publisher
Ikeda K, Horie Inoue K, Suzuki T, Hobo R, Nakasato N, Takeda S, et al. Mitochondrial supercomplex assembly promotes breast and endometrial tumorigenesis by metabolic alterations and enhanced hypoxia tolerance. Nat Commun. 2019;10:4108 pubmed publisher
Lin S, Huang C, Gunda V, Sun J, Chellappan S, Li Z, et al. Fascin Controls Metastatic Colonization and Mitochondrial Oxidative Phosphorylation by Remodeling Mitochondrial Actin Filaments. Cell Rep. 2019;28:2824-2836.e8 pubmed publisher
Zhang R, Wang X, Qu J, Liu B, Zhang P, Zhang T, et al. Caloric Restriction Induces MicroRNAs to Improve Mitochondrial Proteostasis. iScience. 2019;17:155-166 pubmed publisher
Hubbard W, Harwood C, Prajapati P, Springer J, Saatman K, Sullivan P. Fractionated mitochondrial magnetic separation for isolation of synaptic mitochondria from brain tissue. Sci Rep. 2019;9:9656 pubmed publisher
Deng J, Guo Y, Yuan F, Chen S, Yin H, Jiang X, et al. Autophagy inhibition prevents glucocorticoid-increased adiposity via suppressing BAT whitening. Autophagy. 2019;:1-15 pubmed publisher
Pestoni J, Klingeman Plati S, Valdivia Camacho O, Fuse M, Onatunde M, Sparrow N, et al. Peroxynitrite supports a metabolic reprogramming in merlin-deficient Schwann cells and promotes cell survival. J Biol Chem. 2019;294:11354-11368 pubmed publisher
Nakazawa H, Ikeda K, Shinozaki S, Yasuhara S, Yu Y, Martyn J, et al. Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle. FEBS Open Bio. 2019;9:348-363 pubmed publisher
Katsu Jiménez Y, Vázquez Calvo C, Maffezzini C, Halldin M, Peng X, Freyer C, et al. Absence of TXNIP in Humans Leads to Lactic Acidosis and Low Serum Methionine Linked to Deficient Respiration on Pyruvate. Diabetes. 2019;68:709-723 pubmed publisher
Nadeau L, Patten D, Caron A, Garneau L, Pinault Masson E, Foretz M, et al. IL-15 improves skeletal muscle oxidative metabolism and glucose uptake in association with increased respiratory chain supercomplex formation and AMPK pathway activation. Biochim Biophys Acta Gen Subj. 2019;1863:395-407 pubmed publisher
Sprenger H, Wani G, Hesseling A, König T, Patron M, MacVicar T, et al. Loss of the mitochondrial i-AAA protease YME1L leads to ocular dysfunction and spinal axonopathy. EMBO Mol Med. 2019;11: pubmed publisher
Timper K, Paeger L, Sánchez Lasheras C, Varela L, Jais A, Nolte H, et al. Mild Impairment of Mitochondrial OXPHOS Promotes Fatty Acid Utilization in POMC Neurons and Improves Glucose Homeostasis in Obesity. Cell Rep. 2018;25:383-397.e10 pubmed publisher
Scott A, Walker S, Krank J, Wilkinson A, Johnson K, Lewis E, et al. AIF promotes a JNK1-mediated cadherin switch independently of respiratory chain stabilization. J Biol Chem. 2018;293:14707-14722 pubmed publisher
Hilse K, Rupprecht A, Egerbacher M, Bardakji S, Zimmermann L, Wulczyn A, et al. The Expression of Uncoupling Protein 3 Coincides With the Fatty Acid Oxidation Type of Metabolism in Adult Murine Heart. Front Physiol. 2018;9:747 pubmed publisher
Becker C, Kukat A, Szczepanowska K, Hermans S, Senft K, Brandscheid C, et al. CLPP deficiency protects against metabolic syndrome but hinders adaptive thermogenesis. EMBO Rep. 2018;19: pubmed publisher
Kumar V, Bouameur J, Bär J, Rice R, Hornig Do H, Roop D, et al. A keratin scaffold regulates epidermal barrier formation, mitochondrial lipid composition, and activity. J Cell Biol. 2015;211:1057-75 pubmed publisher
Hilse K, Kalinovich A, Rupprecht A, Smorodchenko A, Zeitz U, Staniek K, et al. The expression of UCP3 directly correlates to UCP1 abundance in brown adipose tissue. Biochim Biophys Acta. 2016;1857:72-78 pubmed publisher
Zhang J, Gao Q, Zhou Y, Dier U, Hempel N, Hochwald S. Focal adhesion kinase-promoted tumor glucose metabolism is associated with a shift of mitochondrial respiration to glycolysis. Oncogene. 2016;35:1926-42 pubmed publisher
product information
Product Type :
Antibody
Product Name :
NDUFA9 Monoclonal Antibody (20C11B11B11)
Catalog # :
459100
Quantity :
100 µg
Price :
US 459
Clonality :
Monoclonal
Purity :
IgG fraction
Host :
Mouse
Reactivity :
Bovine, Human, Mouse, Rat
Applications :
Flow Cytometry: 1-2 µg/10 ^6 cells, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1.0 µg/mL
Species :
Bovine, Human, Mouse, Rat
Clone :
20C11B11B11
Isotype :
IgG1, kappa
Storage :
4° C, do not freeze
Description :
NDUFA9 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial) is also named as NDUFS2L, CI-39kD and belongs to the complex I NDUFA9 subunit family. It is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity.
Immunogen :
Bovine Heart Complex I
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1-2 µg/10 ^6 cells, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1.0 µg/mL
Aliases :
1010001N11Rik; CC6; CI39k; CI-39k; CI-39kD; complex I 39kDa subunit; complex I-39kD; EMBL:AAH91192.1, ECO:0000312; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa; NADH dehydrogenase (ubiquinone) 42 kDa subunit; NADH dehydrogenase (ubiquinone) Fe-S protein 2-like (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; NADH:ubiquinone oxidoreductase subunit A9; NADH-ubiquinone oxidoreductase 39 kDa subunit; NDUFA9; ndufa9 {ECO:0000312; NDUFS2L; RGD:1307307}; SDR22E1; short chain dehydrogenase/reductase family 22E, member 1; sperm flagella protein 3
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA