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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45.1 Monoclonal Antibody (A20), PerCP-Cyanine5.5, eBioscience
catalog :
45-0453-82
quantity :
100 µg
price :
US 339
clonality :
monoclonal
host :
mouse
conjugate :
PerCP/Cy5.5
clone name :
A20
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:300; loading ...; fig 2f, 2g
Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher
  • flow cytometry; mouse; fig s3d
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7d
Smith T, Moffett H, Stephan S, Opel C, Dumigan A, Jiang X, et al. Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors. J Clin Invest. 2017;127:2176-2191 pubmed publisher
  • flow cytometry; mouse
Nowacka J, Baumgartner C, Pelorosso C, Roth M, Zuber J, Baccarini M. MEK1 is required for the development of NRAS-driven leukemia. Oncotarget. 2016;7:80113-80130 pubmed publisher
  • flow cytometry; mouse; fig s1
van Blijswijk J, Schraml B, Rogers N, Whitney P, Zelenay S, Acton S, et al. Altered lymph node composition in diphtheria toxin receptor-based mouse models to ablate dendritic cells. J Immunol. 2015;194:307-15 pubmed publisher
  • flow cytometry; mouse; fig 6b
Wu X, Satpathy A, Kc W, Liu P, Murphy T, Murphy K. Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo. PLoS ONE. 2013;8:e64800 pubmed publisher
Khouili S, Cook E, Hernández García E, Martínez López M, Conde Garrosa R, Iborra S. SHP-1 Regulates Antigen Cross-Presentation and Is Exploited by Leishmania to Evade Immunity. Cell Rep. 2020;33:108468 pubmed publisher
Köchl R, Vanes L, Llorian Sopena M, Chakravarty P, Hartweger H, Fountain K, et al. Critical role of WNK1 in MYC-dependent early mouse thymocyte development. elife. 2020;9: pubmed publisher
Khiew S, Jain D, Chen J, Yang J, Yin D, Young J, et al. Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells. J Clin Invest. 2020;130:3453-3466 pubmed publisher
B guelin W, Teater M, Meydan C, Hoehn K, Phillip J, Soshnev A, et al. Mutant EZH2 Induces a Pre-malignant Lymphoma Niche by Reprogramming the Immune Response. Cancer Cell. 2020;37:655-673.e11 pubmed publisher
Rana A, de Almeida F, Paico Montero H, Gonzales Carazas M, Bortoluci K, Sad S, et al. RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes. Front Immunol. 2020;11:536 pubmed publisher
Zenke S, Palm M, Braun J, Gavrilov A, Meiser P, Böttcher J, et al. Quorum Regulation via Nested Antagonistic Feedback Circuits Mediated by the Receptors CD28 and CTLA-4 Confers Robustness to T Cell Population Dynamics. Immunity. 2020;52:313-327.e7 pubmed publisher
Gall M, Zhang H, Lee Q, Jolly C, McCaughan G, Cook A, et al. Immune regeneration in irradiated mice is not impaired by the absence of DPP9 enzymatic activity. Sci Rep. 2019;9:7292 pubmed publisher
Beneke V, Küster F, Neehus A, Hesse C, Lopez Rodriguez E, Haake K, et al. An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. Sci Rep. 2018;8:16281 pubmed publisher
Kim M, Galan C, Hill A, Wu W, Fehlner Peach H, Song H, et al. Critical Role for the Microbiota in CX3CR1+ Intestinal Mononuclear Phagocyte Regulation of Intestinal T Cell Responses. Immunity. 2018;49:151-163.e5 pubmed publisher
Schmidt K, Zhang Q, Tasdogan A, Petzold A, Dahl A, Arneth B, et al. The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice. elife. 2018;7: pubmed publisher
Xia P, Wang S, Ye B, Du Y, Li C, Xiong Z, et al. A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion. Immunity. 2018;48:688-701.e7 pubmed publisher
Zhang B, Nguyen L, Li L, Zhao D, Kumar B, Wu H, et al. Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia. Nat Med. 2018;24:450-462 pubmed publisher
Cimmino L, Dolgalev I, Wang Y, Yoshimi A, Martin G, Wang J, et al. Restoration of TET2 Function Blocks Aberrant Self-Renewal and Leukemia Progression. Cell. 2017;170:1079-1095.e20 pubmed publisher
Miao T, Symonds A, Singh R, Symonds J, Ogbe A, Omodho B, et al. Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation. J Exp Med. 2017;214:1787-1808 pubmed publisher
Lewis G, Wehrens E, Labarta Bajo L, Streeck H, Zuniga E. TGF-? receptor maintains CD4 T helper cell identity during chronic viral infections. J Clin Invest. 2016;126:3799-3813 pubmed publisher
Drennan M, Govindarajan S, Verheugen E, Coquet J, Staal J, McGuire C, et al. NKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20. J Exp Med. 2016;213:1973-81 pubmed publisher
Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
Mucci A, Kunkiel J, Suzuki T, Brennig S, Glage S, Kühnel M, et al. Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency. Stem Cell Reports. 2016;7:292-305 pubmed publisher
Srikakulapu P, Hu D, Yin C, Mohanta S, Bontha S, Peng L, et al. Artery Tertiary Lymphoid Organs Control Multilayered Territorialized Atherosclerosis B-Cell Responses in Aged ApoE-/- Mice. Arterioscler Thromb Vasc Biol. 2016;36:1174-85 pubmed publisher
Ghosh M, Shapiro L. In vitro Ag Cross-presentation and in vivo Ag Cross-presentation by Dendritic Cells in the Mouse. Bio Protoc. 2012;2:e305 pubmed
Wu L, Li N, Zhang M, Xue S, Cassady K, Lin Q, et al. MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice. Proc Natl Acad Sci U S A. 2015;112:15994-9 pubmed publisher
Xue J, Sharma V, Hsieh M, Chawla A, Murali R, Pandol S, et al. Alternatively activated macrophages promote pancreatic fibrosis in chronic pancreatitis. Nat Commun. 2015;6:7158 pubmed publisher
Basu R, Whitley S, Bhaumik S, Zindl C, Schoeb T, Benveniste E, et al. IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance. Nat Immunol. 2015;16:286-95 pubmed publisher
Xia P, Wang S, Huang G, Zhu P, Li M, Ye B, et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med. 2014;211:2119-34 pubmed publisher
Haldar M, Kohyama M, So A, Kc W, Wu X, Briseño C, et al. Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages. Cell. 2014;156:1223-1234 pubmed publisher
Grosselin J, Sii Felice K, Payen E, Chretien S, Tronik Le Roux D, Leboulch P. Arrayed lentiviral barcoding for quantification analysis of hematopoietic dynamics. Stem Cells. 2013;31:2162-71 pubmed publisher
Zhang H, Wang Y, Meng A, Yan H, WANG X, Niu J, et al. Inhibiting TGF?1 has a protective effect on mouse bone marrow suppression following ionizing radiation exposure in vitro. J Radiat Res. 2013;54:630-6 pubmed publisher
Reis B, Rogoz A, Costa Pinto F, Taniuchi I, Mucida D. Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4? T cell immunity. Nat Immunol. 2013;14:271-80 pubmed publisher
Ma D, Suthar M, Kasahara S, Gale M, Clark E. CD22 is required for protection against West Nile virus Infection. J Virol. 2013;87:3361-75 pubmed publisher
Taylor J, Pape K, Jenkins M. A germinal center-independent pathway generates unswitched memory B cells early in the primary response. J Exp Med. 2012;209:597-606 pubmed publisher
Greenbaum A, Revollo L, Woloszynek J, Civitelli R, Link D. N-cadherin in osteolineage cells is not required for maintenance of hematopoietic stem cells. Blood. 2012;120:295-302 pubmed publisher
Wojno E, Hosken N, Stumhofer J, O Hara A, Mauldin E, Fang Q, et al. A role for IL-27 in limiting T regulatory cell populations. J Immunol. 2011;187:266-73 pubmed publisher
product information
Product Type :
Antibody
Product Name :
CD45.1 Monoclonal Antibody (A20), PerCP-Cyanine5.5, eBioscience
Catalog # :
45-0453-82
Quantity :
100 µg
Price :
US 339
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.25 µg/test
Species :
Mouse
Clone :
A20
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.25 µg/test
Aliases :
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA