product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-IRS1 (Tyr612) Polyclonal Antibody
catalog :
44-816G
quantity :
100 µL
price :
US 500.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; fig 6e
Lertpatipanpong P, Lee J, Kim I, Eling T, Oh S, Seong J, et al. The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice. Sci Rep. 2021;11:15027 pubmed publisher
  • western blot; mouse; loading ...; fig 4i
Wang X, Cao Q, Yu L, Shi H, Xue B, Shi H. Epigenetic regulation of macrophage polarization and inflammation by DNA methylation in obesity. JCI Insight. 2016;1:e87748 pubmed publisher
  • western blot; human; fig 3
Leonard S, Kinsella G, Benetti E, Findlay J. Regulating the effects of GPR21, a novel target for type 2 diabetes. Sci Rep. 2016;6:27002 pubmed publisher
Gayen M, Benoit M, Fan Q, Hudobenko J, Yan R. The CX3CL1 intracellular domain exhibits neuroprotection via insulin receptor/insulin-like growth factor receptor signaling. J Biol Chem. 2022;298:102532 pubmed publisher
Chen C, Gu L, Matye D, Clayton Y, Hasan M, Wang Y, et al. Cullin neddylation inhibitor attenuates hyperglycemia by enhancing hepatic insulin signaling through insulin receptor substrate stabilization. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher
Kanakkanthara A, Hou X, Ekstrom T, Zanfagnin V, Huehls A, Kelly R, et al. Repurposing Ceritinib Induces DNA Damage and Enhances PARP Inhibitor Responses in High-Grade Serous Ovarian Carcinoma. Cancer Res. 2022;82:307-319 pubmed publisher
Mehta S, Fiorelli R, Bao X, Pennington Krygier C, Derogatis A, Kim S, et al. A Phase 0 Trial of Ceritinib in Patients with Brain Metastases and Recurrent Glioblastoma. Clin Cancer Res. 2022;28:289-297 pubmed publisher
Vieira R, Mu xf1 oz V, Junqueira R, de Oliveira F, Gaspar R, Nakandakari S, et al. Time-restricted feeding combined with aerobic exercise training can prevent weight gain and improve metabolic disorders in mice fed a high-fat diet. J Physiol. 2022;600:797-813 pubmed publisher
You D, Chul Jung B, Villivalam S, Lim H, Kang S. JMJD8 is a Novel Molecular Nexus Between Adipocyte-Intrinsic Inflammation and Insulin Resistance. Diabetes. 2021;: pubmed publisher
Muñoz V, Gaspar R, Severino M, Macêdo A, Simabuco F, Ropelle E, et al. Exercise Counterbalances Rho/ROCK2 Signaling Impairment in the Skeletal Muscle and Ameliorates Insulin Sensitivity in Obese Mice. Front Immunol. 2021;12:702025 pubmed publisher
Kuramoto K, Kim Y, Hong J, He C. The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding. Cell Rep. 2021;35:109184 pubmed publisher
Yu W, Singh R, Wang Z, O Malley B, Yi P. The E3 ligase TRAF4 promotes IGF signaling by mediating atypical ubiquitination of IRS-1. J Biol Chem. 2021;296:100739 pubmed publisher
Liu Y, Tang L, Liang C, Wu M, Zhang Z. Insulin Resistance and Pellino-1 Mediated Decrease in the Activities of Vasodilator Signaling Contributes to Sunitinib-Induced Hypertension. Front Pharmacol. 2021;12:617165 pubmed publisher
Shatoor A, Al Humayed S, Almohiy H. Short-term administration of C. aronia stimulates insulin signaling, suppresses fatty acids metabolism, and increases glucose uptake and utilization in the hearts of healthy rats. Saudi J Biol Sci. 2021;28:1966-1977 pubmed publisher
Jo D, Yoon G, Song J. Role of Exendin-4 in Brain Insulin Resistance, Mitochondrial Function, and Neurite Outgrowth in Neurons under Palmitic Acid-Induced Oxidative Stress. Antioxidants (Basel). 2021;10: pubmed publisher
Bai Y, Du R, Tang L, Li L. The role of Smad4 in the regulation of insulin resistance, inflammation and cell proliferation in HTR8-Svneo cells. Cell Biochem Funct. 2021;39:126-138 pubmed publisher
Chou S, Chan L, Chung C, Chiu J, Hsieh Y, Hong C. Altered Insulin Receptor Substrate 1 Phosphorylation in Blood Neuron-Derived Extracellular Vesicles From Patients With Parkinson's Disease. Front Cell Dev Biol. 2020;8:564641 pubmed publisher
Filippello A, Scamporrino A, Di Mauro S, Malaguarnera R, Di Pino A, Scicali R, et al. Direct Effects of D-Chiro-Inositol on Insulin Signaling and Glucagon Secretion of Pancreatic Alpha Cells. Biomolecules. 2020;10: pubmed publisher
Lv H, Tang L, Guo C, Jiang Y, Gao C, Wang Y, et al. Intranasal insulin administration may be highly effective in improving cognitive function in mice with cognitive dysfunction by reversing brain insulin resistance. Cogn Neurodyn. 2020;14:323-338 pubmed publisher
Hernandez D, Kang J, Choudhury M, Andrianifahanana M, Yin X, Limper A, et al. IPF pathogenesis is dependent upon TGFβ induction of IGF-1. FASEB J. 2020;34:5363-5388 pubmed publisher
Jahng J, Alsaadi R, Palanivel R, Song E, Hipolito V, Sung H, et al. Iron overload inhibits late stage autophagic flux leading to insulin resistance. EMBO Rep. 2019;20:e47911 pubmed publisher
Luo Q, Xiao Y, Alex A, Cummins T, Bhatwadekar A. The Diurnal Rhythm of Insulin Receptor Substrate-1 (IRS-1) and Kir4.1 in Diabetes: Implications for a Clock Gene Bmal1. Invest Ophthalmol Vis Sci. 2019;60:1928-1936 pubmed publisher
Nakazawa H, Ikeda K, Shinozaki S, Yasuhara S, Yu Y, Martyn J, et al. Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle. FEBS Open Bio. 2019;9:348-363 pubmed publisher
Bian F, Ma X, Villivalam S, You D, Choy L, Paladugu A, et al. TET2 facilitates PPARγ agonist-mediated gene regulation and insulin sensitization in adipocytes. Metabolism. 2018;89:39-47 pubmed publisher
Woo H, Hong C, Jung S, Choe S, Yu S. Chronic restraint stress induces hippocampal memory deficits by impairing insulin signaling. Mol Brain. 2018;11:37 pubmed publisher
Lassak A, Dean M, Wyczechowska D, Wilk A, Marrero L, Trillo Tinoco J, et al. Molecular and Structural Traits of Insulin Receptor Substrate 1/LC3 Nuclear Structures and Their Role in Autophagy Control and Tumor Cell Survival. Mol Cell Biol. 2018;38: pubmed publisher
Holt L, Brandon A, Small L, Suryana E, Preston E, Wilks D, et al. Ablation of Grb10 Specifically in Muscle Impacts Muscle Size and Glucose Metabolism in Mice. Endocrinology. 2018;159:1339-1351 pubmed publisher
You D, Nilsson E, Tenen D, Lyubetskaya A, Lo J, Jiang R, et al. Dnmt3a is an epigenetic mediator of adipose insulin resistance. elife. 2017;6: pubmed publisher
Marciniak E, Leboucher A, Caron E, Ahmed T, Tailleux A, Dumont J, et al. Tau deletion promotes brain insulin resistance. J Exp Med. 2017;214:2257-2269 pubmed publisher
Meex R, Hoy A, Morris A, Brown R, Lo J, Burke M, et al. Fetuin B Is a Secreted Hepatocyte Factor Linking Steatosis to Impaired Glucose Metabolism. Cell Metab. 2015;22:1078-89 pubmed publisher
Garwood C, Ratcliffe L, Morgan S, Simpson J, Owens H, Vazquez Villaseñor I, et al. Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors. Mol Brain. 2015;8:51 pubmed publisher
Meyer K, Albaugh B, Schoenike B, Roopra A. Type 1 Insulin-Like Growth Factor Receptor/Insulin Receptor Substrate 1 Signaling Confers Pathogenic Activity on Breast Tumor Cells Lacking REST. Mol Cell Biol. 2015;35:2991-3004 pubmed publisher
Balhara B, Burkart A, Topcu V, Lee Y, Cowan C, Kahn C, et al. Severe insulin resistance alters metabolism in mesenchymal progenitor cells. Endocrinology. 2015;156:2039-48 pubmed publisher
Nakazawa H, Yamada M, Tanaka T, Kramer J, Yu Y, Fischman A, et al. Role of protein farnesylation in burn-induced metabolic derangements and insulin resistance in mouse skeletal muscle. PLoS ONE. 2015;10:e0116633 pubmed publisher
Isoyama S, Kajiwara G, Tamaki N, Okamura M, Yoshimi H, Nakamura N, et al. Basal expression of insulin-like growth factor 1 receptor determines intrinsic resistance of cancer cells to a phosphatidylinositol 3-kinase inhibitor ZSTK474. Cancer Sci. 2015;106:171-8 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-IRS1 (Tyr612) Polyclonal Antibody
Catalog # :
44-816G
Quantity :
100 µL
Price :
US 500.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Human
Applications :
Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000
Species :
Human
Isotype :
IgG
Storage :
-20°C
Description :
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
Immunogen :
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IRS-1 that contains tyrosine 612. The sequence is conserved in mouse, rat and chicken.
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000
Aliases :
G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA