Phospho-Tau (Ser199, Ser202) Polyclonal Antibody | Invitrogen 44-768G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Tau (Ser199, Ser202) Polyclonal Antibody

catalog :

44-768G

quantity :

100 µL

price :

US 514

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
western blot; mouse western blot knockout validation; human; 1:500; tbl 1 immunocytochemistry; human; 1:100; tbl 2	Ercan E, Eid S, Weber C, Kowalski A, Bichmann M, Behrendt A, et al. A validated antibody panel for the characterization of tau post-translational modifications. Mol Neurodegener. 2017;12:87 pubmed publisher
ELISA; human; loading ...; fig 1a	Ercan Herbst E, Ehrig J, Schöndorf D, Behrendt A, Klaus B, Gomez Ramos B, et al. A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer's disease brain. Acta Neuropathol Commun. 2019;7:192 pubmed publisher
western blot; mouse; loading ...; fig e1a	Faraco G, Hochrainer K, Segarra S, Schaeffer S, Santisteban M, Menon A, et al. Dietary salt promotes cognitive impairment through tau phosphorylation. Nature. 2019;: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2A	Chen S, Sun J, Zhao G, Guo A, Chen Y, Fu R, et al. Liraglutide Improves Water Maze Learning and Memory Performance While Reduces Hyperphosphorylation of Tau and Neurofilaments in APP/PS1/Tau Triple Transgenic Mice. Neurochem Res. 2017;42:2326-2335 pubmed publisher
immunohistochemistry - paraffin section; human; 1:5000; loading ...; fig 6	Noy S, Krawitz S, Del Bigio M. Chronic Traumatic Encephalopathy-Like Abnormalities in a Routine Neuropathology Service. J Neuropathol Exp Neurol. 2016;75:1145-1154 pubmed publisher
	Kim Y, Siddiqui T, Blaze J, Cosacak M, Winters T, Kumar A, et al. RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation. Acta Neuropathol. 2023;145:29-48 pubmed publisher
	Yan F, Liu J, Chen M, Zhang Y, Wei S, Jin H, et al. Icariin ameliorates memory deficits through regulating brain insulin signaling and glucose transporters in 3×Tg-AD mice. Neural Regen Res. 2023;18:183-188 pubmed publisher
	Levert S, Pilliod J, Aumont x, Armanville S, Tremblay C, Calon F, et al. Direct and Indirect Effects of Filamin A on Tau Pathology in Neuronal Cells. Mol Neurobiol. 2022;: pubmed publisher
	Tan B, Babür E, Toy N, G xfc nayd x131 n B, Dursun N, S xfc er C. Tau protein is differentially phosphorylated in young- and old-aged rats with experimentally induced hyperthyroidism. Int J Dev Neurosci. 2022;82:654-663 pubmed publisher
	Gannon O, Robison L, Salinero A, Abi Ghanem C, Mansour F, Kelly R, et al. High-fat diet exacerbates cognitive decline in mouse models of Alzheimer's disease and mixed dementia in a sex-dependent manner. J Neuroinflammation. 2022;19:110 pubmed publisher
	Brody A, Nies S, Guan F, Smith L, Mukherjee B, Salazar S, et al. Alzheimer risk gene product Pyk2 suppresses tau phosphorylation and phenotypic effects of tauopathy. Mol Neurodegener. 2022;17:32 pubmed publisher
	Wang X, Qi L, Cheng Y, Ji X, Chi T, Liu P, et al. PINK1 overexpression prevents forskolin-induced tau hyperphosphorylation and oxidative stress in a rat model of Alzheimer's disease. Acta Pharmacol Sin. 2022;43:1916-1927 pubmed publisher
	Hong S, Nagayach A, Lu Y, Peng H, Duong Q, Pham N, et al. A high fat, sugar, and salt Western diet induces motor-muscular and sensory dysfunctions and neurodegeneration in mice during aging: Ameliorative action of metformin. CNS Neurosci Ther. 2021;27:1458-1471 pubmed publisher
	Alavi M. Tau phosphorylation and OPA1 proteolysis are unrelated events: Implications for Alzheimer's Disease. Biochim Biophys Acta Mol Cell Res. 2021;1868:119116 pubmed publisher
	Wijesekara N, Gon xe7 alves R, Ahrens R, Ha K, De Felice F, Fraser P. Combination of human tau and islet amyloid polypeptide exacerbates metabolic dysfunction in transgenic mice. J Pathol. 2021;254:244-253 pubmed publisher
	Reimer L, Betzer C, Kofoed R, Volbracht C, Fog K, Kurhade C, et al. PKR kinase directly regulates tau expression and Alzheimer's disease-related tau phosphorylation. Brain Pathol. 2021;31:103-119 pubmed publisher
	Pilliod J, Desjardins A, Pernègre C, Jamann H, Larochelle C, Fon E, et al. Clearance of intracellular tau protein from neuronal cells via VAMP8-induced secretion. J Biol Chem. 2020;295:17827-17841 pubmed publisher
	Chiasseu M, Fesharaki Zadeh A, Saito T, Saido T, Strittmatter S. Gene-environment interaction promotes Alzheimer's risk as revealed by synergy of repeated mild traumatic brain injury and mouse App knock-in. Neurobiol Dis. 2020;145:105059 pubmed publisher
	Heckmann B, Teubner B, Boada Romero E, Tummers B, Guy C, Fitzgerald P, et al. Noncanonical function of an autophagy protein prevents spontaneous Alzheimer's disease. Sci Adv. 2020;6:eabb9036 pubmed publisher
	Drummond E, Pires G, MacMurray C, Askenazi M, Nayak S, Bourdon M, et al. Phosphorylated tau interactome in the human Alzheimer's disease brain. Brain. 2020;143:2803-2817 pubmed publisher
	Schwab N, Grenier K, Hazrati L. DNA repair deficiency and senescence in concussed professional athletes involved in contact sports. Acta Neuropathol Commun. 2019;7:182 pubmed publisher
	Heckmann B, Teubner B, Tummers B, Boada Romero E, Harris L, Yang M, et al. LC3-Associated Endocytosis Facilitates β-Amyloid Clearance and Mitigates Neurodegeneration in Murine Alzheimer's Disease. Cell. 2019;: pubmed publisher
	Schwab N, Tator C, Hazrati L. DNA damage as a marker of brain damage in individuals with history of concussions. Lab Invest. 2019;: pubmed publisher
	Mohamed N, Desjardins A, Leclerc N. Tau secretion is correlated to an increase of Golgi dynamics. PLoS ONE. 2017;12:e0178288 pubmed publisher
	Rodriguez L, Mohamed N, Desjardins A, Lippé R, Fon E, Leclerc N. Rab7A regulates tau secretion. J Neurochem. 2017;141:592-605 pubmed publisher
	Blas Rus N, Bustos Morán E, Perez de Castro I, de Carcer G, Borroto A, Camafeita E, et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun. 2016;7:11389 pubmed publisher
	Kaufman A, Salazar S, Haas L, Yang J, Kostylev M, Jeng A, et al. Fyn inhibition rescues established memory and synapse loss in Alzheimer mice. Ann Neurol. 2015;77:953-71 pubmed publisher
	Zhang J, Tan H, Jiang W, Zuo Z. The choice of general anesthetics may not affect neuroinflammation and impairment of learning and memory after surgery in elderly rats. J Neuroimmune Pharmacol. 2015;10:179-89 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-Tau (Ser199, Ser202) Polyclonal Antibody

Catalog # :

44-768G

Quantity :

100 µL

Price :

US 514

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000

Species :

Human, Mouse

Isotype :

IgG

Storage :

-20°C

Description :

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen :

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human tau that contains serines 199 and 202. The sequence is conserved in mouse and rat.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000

Aliases :

AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)

more info or order :

Invitrogen product webpage