Phospho-Tau (Ser422) Polyclonal Antibody | Invitrogen 44-764G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Tau (Ser422) Polyclonal Antibody

catalog :

44-764G

quantity :

100 µL

price :

US 446

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, fruit fly

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - free floating section

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 5g	Andr xe9 s Benito P, Carmona M, Jord xe1 n M, Fern xe1 ndez Irigoyen J, Santamar xed a E, Del Rio J, et al. Host Tau Genotype Specifically Designs and Regulates Tau Seeding and Spreading and Host Tau Transformation Following Intrahippocampal Injection of Identical Tau AD Inoculum. Int J Mol Sci. 2022;23: pubmed publisher
western blot; mouse; 1:1000; fig 3	Manassero G, Guglielmotto M, Zamfir R, Borghi R, Colombo L, Salmona M, et al. Beta-amyloid 1-42 monomers, but not oligomers, produce PHF-like conformation of Tau protein. Aging Cell. 2016;15:914-23 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:2000; fig 1	Li T, Braunstein K, Zhang J, Lau A, Sibener L, Deeble C, et al. The neuritic plaque facilitates pathological conversion of tau in an Alzheimer's disease mouse model. Nat Commun. 2016;7:12082 pubmed publisher
western blot; fruit fly ; fig 3	Dourlen P, Fernandez Gomez F, Dupont C, Grenier Boley B, Bellenguez C, Obriot H, et al. Functional screening of Alzheimer risk loci identifies PTK2B as an in vivo modulator and early marker of Tau pathology. Mol Psychiatry. 2017;22:874-883 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:250; fig 5	Bouvier D, Jones E, Quesseveur G, Davoli M, A Ferreira T, Quirion R, et al. High Resolution Dissection of Reactive Glial Nets in Alzheimer's Disease. Sci Rep. 2016;6:24544 pubmed publisher
	Van Hummel A, Taleski G, Sontag J, Feiten A, Ke Y, Ittner L, et al. Methyl donor supplementation reduces phospho-Tau, Fyn and demethylated protein phosphatase 2A levels and mitigates learning and motor deficits in a mouse model of tauopathy. Neuropathol Appl Neurobiol. 2023;49:e12931 pubmed publisher
	Salgado B, Sastre I, Bullido M, Aldudo J. Herpes Simplex Virus Type 1 Induces AD-like Neurodegeneration Markers in Human Progenitor and Differentiated ReNcell VM Cells. Microorganisms. 2023;11: pubmed publisher
	Arputhasamy C, Foulger A, Lucanic M, Rane A, Schmidt M, Garrett T, et al. Potassium-chelating drug sodium polystyrene sulfonate enhances lysosomal function and suppresses proteotoxicity. GeroScience. 2023;45:1237-1245 pubmed publisher
	Kim D, Tu K, Wei A, Lau A, Gonzalez Gil A, Cao T, et al. Amyloid-beta and tau pathologies act synergistically to induce novel disease stage-specific microglia subtypes. Mol Neurodegener. 2022;17:83 pubmed publisher
	Li L, Miao J, Chu D, Jin N, Tung Y, Dai C, et al. Tau antibody 77G7 targeting microtubule binding domain suppresses proteopathic tau to seed tau aggregation. CNS Neurosci Ther. 2022;28:2245-2259 pubmed publisher
	Fixemer S, Ameli C, Hammer G, Salamanca L, Uriarte Huarte O, Schwartz C, et al. Microglia phenotypes are associated with subregional patterns of concomitant tau, amyloid-β and α-synuclein pathologies in the hippocampus of patients with Alzheimer's disease and dementia with Lewy bodies. Acta Neuropathol Commun. 2022;10:36 pubmed publisher
	Chen L, van Zijl P, Wei Z, Lu H, Duan W, Wong P, et al. Early detection of Alzheimer's disease using creatine chemical exchange saturation transfer magnetic resonance imaging. Neuroimage. 2021;236:118071 pubmed publisher
	Martinez Valbuena I, Valenti Azcarate R, Amat Villegas I, Marcilla I, Martí Andrés G, Caballero M, et al. Mixed pathologies in pancreatic β cells from subjects with neurodegenerative diseases and their interaction with prion protein. Acta Neuropathol Commun. 2021;9:64 pubmed publisher
	Ruan Z, Pathak D, Venkatesan Kalavai S, Yoshii Kitahara A, Muraoka S, Bhatt N, et al. Alzheimer's disease brain-derived extracellular vesicles spread tau pathology in interneurons. Brain. 2021;144:288-309 pubmed publisher
	Guglielmotto M, Manassero G, Vasciaveo V, Venezia M, Tabaton M, Tamagno E. Estrogens Inhibit Amyloid-β-Mediated Paired Helical Filament-Like Conformation of Tau Through Antioxidant Activity and miRNA 218 Regulation in hTau Mice. J Alzheimers Dis. 2020;77:1339-1351 pubmed publisher
	Martinez Valbuena I, Valenti Azcarate R, Amat Villegas I, Riverol M, Marcilla I, de Andrea C, et al. Amylin as a potential link between type 2 diabetes and alzheimer disease. Ann Neurol. 2019;86:539-551 pubmed publisher
	Tiernan C, Ginsberg S, He B, Ward S, Guillozet Bongaarts A, Kanaan N, et al. Pretangle pathology within cholinergic nucleus basalis neurons coincides with neurotrophic and neurotransmitter receptor gene dysregulation during the progression of Alzheimer's disease. Neurobiol Dis. 2018;117:125-136 pubmed publisher
	Rodriguez L, Mohamed N, Desjardins A, Lippé R, Fon E, Leclerc N. Rab7A regulates tau secretion. J Neurochem. 2017;141:592-605 pubmed publisher
	Takeda S, Wegmann S, Cho H, DeVos S, Commins C, Roe A, et al. Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer's disease brain. Nat Commun. 2015;6:8490 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-Tau (Ser422) Polyclonal Antibody

Catalog # :

44-764G

Quantity :

100 µL

Price :

US 446

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human

Applications :

Western Blot: 1:1,000

Species :

Human

Isotype :

IgG

Storage :

-20°C

Description :

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen :

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Tau that contains serine 422. The sequence is conserved in mouse and rat.

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1:1,000

Aliases :

AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)

more info or order :

Invitrogen product webpage