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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-Tau (Ser404) Polyclonal Antibody
catalog :
44-758G
quantity :
100 µL
price :
US 466
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, rat, dogs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 28
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 3
García Escudero V, Ruiz Gabarre D, Gargini R, Perez M, Garcia E, Cuadros R, et al. A new non-aggregative splicing isoform of human Tau is decreased in Alzheimer's disease. Acta Neuropathol. 2021;142:159-177 pubmed publisher
  • ELISA; human; loading ...; fig 1a
Ercan Herbst E, Ehrig J, Schöndorf D, Behrendt A, Klaus B, Gomez Ramos B, et al. A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer's disease brain. Acta Neuropathol Commun. 2019;7:192 pubmed publisher
  • immunocytochemistry; human; 1:100; tbl 2
  • western blot; human; 1:500; tbl 1
  • western blot; mouse; 1:100
Ercan E, Eid S, Weber C, Kowalski A, Bichmann M, Behrendt A, et al. A validated antibody panel for the characterization of tau post-translational modifications. Mol Neurodegener. 2017;12:87 pubmed publisher
  • western blot; mouse; loading ...; fig 2e
Bodea L, Evans H, Van der Jeugd A, Ittner L, Delerue F, Kril J, et al. Accelerated aging exacerbates a pre-existing pathology in a tau transgenic mouse model. Aging Cell. 2017;16:377-386 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 7a
Takahashi H, Klein Z, Bhagat S, Kaufman A, Kostylev M, Ikezu T, et al. Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network. Acta Neuropathol. 2017;133:785-807 pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Zimova I, Brezovakova V, Hromádka T, Weisová P, Cubinkova V, Valachova B, et al. Human Truncated Tau Induces Mature Neurofibrillary Pathology in a Mouse Model of Human Tauopathy. J Alzheimers Dis. 2016;54:831-43 pubmed publisher
  • western blot; rat
  • western blot; mouse
  • western blot; dogs; 1:1000; fig 7
Smolek T, Madari A, Farbáková J, Kandrac O, Jadhav S, Cente M, et al. Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment. J Comp Neurol. 2016;524:874-95 pubmed publisher
Car xfa s Cadavieco M, Berenguer L xf3 pez I, Montoro Canelo A, Serrano Lope M, Gonz xe1 lez de la Fuente S, Aguado B, et al. Cognitive decline in diabetic mice predisposed to Alzheimer's disease is greater than in wild type. Life Sci Alliance. 2023;6: pubmed publisher
Espinosa Jim xe9 nez T, Cano A, S xe1 nchez L xf3 pez E, Olloquequi J, Folch J, Bull xf3 M, et al. A novel rhein-huprine hybrid ameliorates disease-modifying properties in preclinical mice model of Alzheimer's disease exacerbated with high fat diet. Cell Biosci. 2023;13:52 pubmed publisher
Brigas H, Ribeiro M, Coelho J, Gomes R, Gomez Murcia V, Carvalho K, et al. IL-17 triggers the onset of cognitive and synaptic deficits in early stages of Alzheimer's disease. Cell Rep. 2021;36:109574 pubmed publisher
Perez Garcia G, De Gasperi R, Gama Sosa M, Perez G, Otero Pagan A, Pryor D, et al. Laterality and region-specific tau phosphorylation correlate with PTSD-related behavioral traits in rats exposed to repetitive low-level blast. Acta Neuropathol Commun. 2021;9:33 pubmed publisher
Reimer L, Betzer C, Kofoed R, Volbracht C, Fog K, Kurhade C, et al. PKR kinase directly regulates tau expression and Alzheimer's disease-related tau phosphorylation. Brain Pathol. 2021;31:103-119 pubmed publisher
Gu J, Xu W, Jin N, Li L, Zhou Y, Chu D, et al. Truncation of Tau selectively facilitates its pathological activities. J Biol Chem. 2020;295:13812-13828 pubmed publisher
Sun Y, Guo Y, Feng X, Jia M, Ai N, Dong Y, et al. The behavioural and neuropathologic sexual dimorphism and absence of MIP-3α in tau P301S mouse model of Alzheimer's disease. J Neuroinflammation. 2020;17:72 pubmed publisher
Schueller E, Paiva I, Blanc F, Wang X, Cassel J, Boutillier A, et al. Dysregulation of histone acetylation pathways in hippocampus and frontal cortex of Alzheimer's disease patients. Eur Neuropsychopharmacol. 2020;33:101-116 pubmed publisher
Chen M, Zheng J, Liu G, Zeng C, Xu E, Zhu W, et al. High Dietary Iron Disrupts Iron Homeostasis and Induces Amyloid-β and Phospho-τ Expression in the Hippocampus of Adult Wild-Type and APP/PS1 Transgenic Mice. J Nutr. 2019;149:2247-2254 pubmed publisher
Martín Maestro P, Gargini R, Garcia E, Simon D, Avila J, García Escudero V. Mitophagy Failure in APP and Tau Overexpression Model of Alzheimer's Disease. J Alzheimers Dis. 2019;: pubmed publisher
Miao J, Shi R, Li L, Chen F, Zhou Y, Tung Y, et al. Pathological Tau From Alzheimer's Brain Induces Site-Specific Hyperphosphorylation and SDS- and Reducing Agent-Resistant Aggregation of Tau in vivo. Front Aging Neurosci. 2019;11:34 pubmed publisher
Ulrich G, Salvade A, Boersema P, Cali T, Foglieni C, Sola M, et al. Phosphorylation of nuclear Tau is modulated by distinct cellular pathways. Sci Rep. 2018;8:17702 pubmed publisher
Chatterjee S, Cassel R, Schneider Anthony A, Merienne K, Cosquer B, Tzeplaeff L, et al. Reinstating plasticity and memory in a tauopathy mouse model with an acetyltransferase activator. EMBO Mol Med. 2018;10: pubmed publisher
Huang C, Irwin M, Wong G, Chang R. Evidence of the impact of systemic inflammation on neuroinflammation from a non-bacterial endotoxin animal model. J Neuroinflammation. 2018;15:147 pubmed publisher
Zhou Y, Shi J, Chu D, Hu W, Guan Z, Gong C, et al. Relevance of Phosphorylation and Truncation of Tau to the Etiopathogenesis of Alzheimer's Disease. Front Aging Neurosci. 2018;10:27 pubmed publisher
Marciniak E, Leboucher A, Caron E, Ahmed T, Tailleux A, Dumont J, et al. Tau deletion promotes brain insulin resistance. J Exp Med. 2017;214:2257-2269 pubmed publisher
Mohamed N, Desjardins A, Leclerc N. Tau secretion is correlated to an increase of Golgi dynamics. PLoS ONE. 2017;12:e0178288 pubmed publisher
Wang Y, Zhang Y, Hu W, Xie S, Gong C, Iqbal K, et al. Rapid alteration of protein phosphorylation during postmortem: implication in the study of protein phosphorylation. Sci Rep. 2015;5:15709 pubmed publisher
Takeda S, Wegmann S, Cho H, DeVos S, Commins C, Roe A, et al. Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer's disease brain. Nat Commun. 2015;6:8490 pubmed publisher
Porquet D, Andrés Benito P, Griñán Ferré C, Camins A, Ferrer I, Canudas A, et al. Amyloid and tau pathology of familial Alzheimer's disease APP/PS1 mouse model in a senescence phenotype background (SAMP8). Age (Dordr). 2015;37:9747 pubmed publisher
Dammer E, Lee A, Duong D, Gearing M, Lah J, Levey A, et al. Quantitative phosphoproteomics of Alzheimer's disease reveals cross-talk between kinases and small heat shock proteins. Proteomics. 2015;15:508-519 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-Tau (Ser404) Polyclonal Antibody
Catalog # :
44-758G
Quantity :
100 µL
Price :
US 466
Clonality :
Polyclonal
Purity :
Affinity chromatography
Host :
Rabbit
Reactivity :
Human, Mouse, Rat
Applications :
Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000
Species :
Human, Mouse, Rat
Isotype :
IgG
Storage :
-20°C
Description :
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Immunogen :
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Tau that contains serine 404. The sequence is conserved in mouse and rat.
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000
Aliases :
AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA