Phospho-Tau (Ser214) Polyclonal Antibody | Invitrogen 44-742G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Tau (Ser214) Polyclonal Antibody

catalog :

44-742G

quantity :

100 µL

price :

US 430.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat, dogs, cat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; cat; 1:1000; loading ...; fig 3e	Fiock K, Smith J, Crary J, Hefti M. β-amyloid and tau pathology in the aging feline brain. J Comp Neurol. 2020;528:108-113 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2A	Chen S, Sun J, Zhao G, Guo A, Chen Y, Fu R, et al. Liraglutide Improves Water Maze Learning and Memory Performance While Reduces Hyperphosphorylation of Tau and Neurofilaments in APP/PS1/Tau Triple Transgenic Mice. Neurochem Res. 2017;42:2326-2335 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 6c	Zimova I, Brezovakova V, Hromádka T, Weisová P, Cubinkova V, Valachova B, et al. Human Truncated Tau Induces Mature Neurofibrillary Pathology in a Mouse Model of Human Tauopathy. J Alzheimers Dis. 2016;54:831-43 pubmed publisher
western blot; human; 1:3000; fig 3	Myeku N, CLELLAND C, Emrani S, Kukushkin N, Yu W, Goldberg A, et al. Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling. Nat Med. 2016;22:46-53 pubmed publisher
western blot; human immunohistochemistry - paraffin section; dogs; fig 3 western blot; dogs; 1:1000; fig 7	Smolek T, Madari A, Farbáková J, Kandrac O, Jadhav S, Cente M, et al. Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment. J Comp Neurol. 2016;524:874-95 pubmed publisher
	Li L, Miao J, Chu D, Jin N, Tung Y, Dai C, et al. Tau antibody 77G7 targeting microtubule binding domain suppresses proteopathic tau to seed tau aggregation. CNS Neurosci Ther. 2022;28:2245-2259 pubmed publisher
	Mate V, Smolek T, Kazmerova Z, Jadhav S, Brezovakova V, Jurkanin B, et al. Enriched environment ameliorates propagation of tau pathology and improves cognition in rat model of tauopathy. Front Aging Neurosci. 2022;14:935973 pubmed publisher
	Farrell K, Kim S, Han N, Iida M, Gonzalez E, Otero García M, et al. Genome-wide association study and functional validation implicates JADE1 in tauopathy. Acta Neuropathol. 2022;143:33-53 pubmed publisher
	Wu R, Li L, Shi R, Zhou Y, Jin N, Gu J, et al. Dephosphorylation Passivates the Seeding Activity of Oligomeric Tau Derived From Alzheimer's Brain. Front Mol Neurosci. 2021;14:631833 pubmed publisher
	Abreha M, Ojelade S, Dammer E, McEachin Z, Duong D, Gearing M, et al. TBK1 interacts with tau and enhances neurodegeneration in tauopathy. J Biol Chem. 2021;296:100760 pubmed publisher
	Gu J, Xu W, Jin N, Li L, Zhou Y, Chu D, et al. Truncation of Tau selectively facilitates its pathological activities. J Biol Chem. 2020;295:13812-13828 pubmed publisher
	Ko H, Chiou S, Wong Y, Wang Y, Lai Y, Chou C, et al. GSKIP-Mediated Anchoring Increases Phosphorylation of Tau by PKA but Not by GSK3beta via cAMP/PKA/GSKIP/GSK3/Tau Axis Signaling in Cerebrospinal Fluid and iPS Cells in Alzheimer Disease. J Clin Med. 2019;8: pubmed publisher
	Hsieh Y, Guo C, Yalamanchili H, Abreha M, Al Ouran R, Li Y, et al. Tau-Mediated Disruption of the Spliceosome Triggers Cryptic RNA Splicing and Neurodegeneration in Alzheimer's Disease. Cell Rep. 2019;29:301-316.e10 pubmed publisher
	Miao J, Shi R, Li L, Chen F, Zhou Y, Tung Y, et al. Pathological Tau From Alzheimer's Brain Induces Site-Specific Hyperphosphorylation and SDS- and Reducing Agent-Resistant Aggregation of Tau in vivo. Front Aging Neurosci. 2019;11:34 pubmed publisher
	Zhang Y, Wu F, Iqbal K, Gong C, Hu W, Liu F. Subacute to chronic Alzheimer-like alterations after controlled cortical impact in human tau transgenic mice. Sci Rep. 2019;9:3789 pubmed publisher
	Zhou Y, Shi J, Chu D, Hu W, Guan Z, Gong C, et al. Relevance of Phosphorylation and Truncation of Tau to the Etiopathogenesis of Alzheimer's Disease. Front Aging Neurosci. 2018;10:27 pubmed publisher
	Hu W, Wu F, Zhang Y, Gong C, Iqbal K, Liu F. Expression of Tau Pathology-Related Proteins in Different Brain Regions: A Molecular Basis of Tau Pathogenesis. Front Aging Neurosci. 2017;9:311 pubmed publisher
	Nagamine S, Yamazaki T, Makioka K, Fujita Y, Ikeda M, Takatama M, et al. Hypersialylation is a common feature of neurofibrillary tangles and granulovacuolar degenerations in Alzheimer's disease and tauopathy brains. Neuropathology. 2016;36:333-45 pubmed publisher
	Wang Y, Zhang Y, Hu W, Xie S, Gong C, Iqbal K, et al. Rapid alteration of protein phosphorylation during postmortem: implication in the study of protein phosphorylation. Sci Rep. 2015;5:15709 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-Tau (Ser214) Polyclonal Antibody

Catalog # :

44-742G

Quantity :

100 µL

Price :

US 430.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1:1,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20°C

Description :

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen :

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 214. The sequence is conserved in many species including mouse, rat, rhesus monkey, baboon, cow and goat.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1:1,000

Aliases :

AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)

more info or order :

Invitrogen product webpage