Phospho-Tau (Thr212) Polyclonal Antibody | Invitrogen 44-740G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Tau (Thr212) Polyclonal Antibody

catalog :

44-740G

quantity :

100 µL

price :

US 446.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

African green monkey, baboons, human, mouse, rat, dogs, bovine, domestic sheep

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4a	Wu X, Kosaraju J, Tam K. SLM, a novel carbazole-based fluorophore attenuates okadaic acid-induced tau hyperphosphorylation via down-regulating GSK-3? activity in SH-SY5Y cells. Eur J Pharm Sci. 2017;110:101-108 pubmed publisher
western blot; mouse; loading ...; fig 3a	Zimova I, Brezovakova V, Hromádka T, Weisová P, Cubinkova V, Valachova B, et al. Human Truncated Tau Induces Mature Neurofibrillary Pathology in a Mouse Model of Human Tauopathy. J Alzheimers Dis. 2016;54:831-43 pubmed publisher
western blot; human; 1:1000; fig 3	Kim H, Lee K, Kim A, Choi M, Choi K, Kang M, et al. A chemical with proven clinical safety rescues Down-syndrome-related phenotypes in through DYRK1A inhibition. Dis Model Mech. 2016;9:839-48 pubmed publisher
western blot; human; 1:3000; fig 1	Kii I, Sumida Y, Goto T, Sonamoto R, Okuno Y, Yoshida S, et al. Selective inhibition of the kinase DYRK1A by targeting its folding process. Nat Commun. 2016;7:11391 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3	Mariano M, Hartmann R, Engel M. Systematic diversification of benzylidene heterocycles yields novel inhibitor scaffolds selective for Dyrk1A, Clk1 and CK2. Eur J Med Chem. 2016;112:209-216 pubmed publisher
immunohistochemistry - paraffin section; dogs western blot; dogs; 1:1000; fig 7 western blot; baboons western blot; human western blot; rat western blot; mouse western blot; domestic sheep western blot; bovine western blot; African green monkey	Smolek T, Madari A, Farbáková J, Kandrac O, Jadhav S, Cente M, et al. Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment. J Comp Neurol. 2016;524:874-95 pubmed publisher
	Li L, Miao J, Chu D, Jin N, Tung Y, Dai C, et al. Tau antibody 77G7 targeting microtubule binding domain suppresses proteopathic tau to seed tau aggregation. CNS Neurosci Ther. 2022;28:2245-2259 pubmed publisher
	Sawa M, OVERK C, Becker A, Derse D, Albay R, Weldy K, et al. Impact of increased APP gene dose in Down syndrome and the Dp16 mouse model. Alzheimers Dement. 2022;18:1203-1234 pubmed publisher
	Wu R, Li L, Shi R, Zhou Y, Jin N, Gu J, et al. Dephosphorylation Passivates the Seeding Activity of Oligomeric Tau Derived From Alzheimer's Brain. Front Mol Neurosci. 2021;14:631833 pubmed publisher
	Lee Y, Im E, Hyun M, Park J, Chung K. Protein phosphatase PPM1B inhibits DYRK1A kinase through dephosphorylation of pS258 and reduces toxic tau aggregation. J Biol Chem. 2021;296:100245 pubmed publisher
	Gu J, Xu W, Jin N, Li L, Zhou Y, Chu D, et al. Truncation of Tau selectively facilitates its pathological activities. J Biol Chem. 2020;295:13812-13828 pubmed publisher
	Lee S, Ko A, Oh Y, Shi P, D Angelo F, Frangaj B, et al. Proline Hydroxylation Primes Protein Kinases for Autophosphorylation and Activation. Mol Cell. 2020;: pubmed publisher
	Trovò L, Fuchs C, De Rosa R, Barbiero I, Tramarin M, Ciani E, et al. The green tea polyphenol epigallocatechin-3-gallate (EGCG) restores CDKL5-dependent synaptic defects in vitro and in vivo. Neurobiol Dis. 2020;138:104791 pubmed publisher
	Miao J, Shi R, Li L, Chen F, Zhou Y, Tung Y, et al. Pathological Tau From Alzheimer's Brain Induces Site-Specific Hyperphosphorylation and SDS- and Reducing Agent-Resistant Aggregation of Tau in vivo. Front Aging Neurosci. 2019;11:34 pubmed publisher
	Czarna A, Wang J, Zelencova D, Liu Y, Deng X, Choi H, et al. Novel Scaffolds for Dual Specificity Tyrosine-Phosphorylation-Regulated Kinase (DYRK1A) Inhibitors. J Med Chem. 2018;61:7560-7572 pubmed publisher
	Hu W, Tung Y, Zhang Y, Liu F, Iqbal K. Involvement of Activation of Asparaginyl Endopeptidase in Tau Hyperphosphorylation in Repetitive Mild Traumatic Brain Injury. J Alzheimers Dis. 2018;64:709-722 pubmed publisher
	Zhou Y, Shi J, Chu D, Hu W, Guan Z, Gong C, et al. Relevance of Phosphorylation and Truncation of Tau to the Etiopathogenesis of Alzheimer's Disease. Front Aging Neurosci. 2018;10:27 pubmed publisher
	Hu W, Wu F, Zhang Y, Gong C, Iqbal K, Liu F. Expression of Tau Pathology-Related Proteins in Different Brain Regions: A Molecular Basis of Tau Pathogenesis. Front Aging Neurosci. 2017;9:311 pubmed publisher
	Wang Y, Zhang Y, Hu W, Xie S, Gong C, Iqbal K, et al. Rapid alteration of protein phosphorylation during postmortem: implication in the study of protein phosphorylation. Sci Rep. 2015;5:15709 pubmed publisher
	RÃ¼ben K, Wurzlbauer A, Walte A, Sippl W, Bracher F, Becker W. Selectivity Profiling and Biological Activity of Novel Î²-Carbolines as Potent and Selective DYRK1 Kinase Inhibitors. PLoS ONE. 2015;10:e0132453 pubmed publisher
	Falke H, Chaikuad A, Becker A, LoaÃ«c N, Lozach O, Abu Jhaisha S, et al. 10-iodo-11H-indolo[3,2-c]quinoline-6-carboxylic acids are selective inhibitors of DYRK1A. J Med Chem. 2015;58:3131-43 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-Tau (Thr212) Polyclonal Antibody

Catalog # :

44-740G

Quantity :

100 µL

Price :

US 446.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1 µg/mL, Western Blot: 1:1,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20°C

Description :

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen :

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains threonine 212. The sequence is conserved in many species including mouse, rat, rhesus monkey, baboon, cow, and goat

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1 µg/mL, Western Blot: 1:1,000

Aliases :

AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)

more info or order :

Invitrogen product webpage