product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-Paxillin (Tyr31) Polyclonal Antibody
catalog :
44-720G
quantity :
100 µL
price :
US 514
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
Invitrogen product webpage
citations: 22
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:100; loading ...; fig 4a
Haroon M, Boers H, Bakker A, Bloks N, Hoogaars W, Giordani L, et al. Reduced growth rate of aged muscle stem cells is associated with impaired mechanosensitivity. Aging (Albany NY). 2022;14:28-53 pubmed publisher
  • western blot; human; loading ...; fig s1d
Nava M, Miroshnikova Y, Biggs L, Whitefield D, Metge F, Boucas J, et al. Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage. Cell. 2020;181:800-817.e22 pubmed publisher
  • western blot; human; 1:500; fig 3b
Wu Y, Jhao Y, Cheng Y, Chen Y. 15-Deoxy-?12,14-prostaglandin J2 inhibits migration of human thyroid carcinoma cells by disrupting focal adhesion complex and adherens junction. Oncol Lett. 2017;13:2569-2576 pubmed publisher
  • immunocytochemistry; mouse; 1:400; fig s3
Kumar A, Ouyang M, van den Dries K, McGhee E, Tanaka K, Anderson M, et al. Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity. J Cell Biol. 2016;213:371-83 pubmed publisher
  • western blot; human; fig 4
Marcos Ramiro B, García Weber D, Barroso S, Feito J, Ortega M, Cernuda Morollón E, et al. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border. J Cell Biol. 2016;213:385-402 pubmed publisher
  • immunocytochemistry; mouse; fig 5
  • western blot; mouse; fig 5
Rooney N, Wang P, Brennan K, Gilmore A, Streuli C. The Integrin-Mediated ILK-Parvin-?Pix Signaling Axis Controls Differentiation in Mammary Epithelial Cells. J Cell Physiol. 2016;231:2408-17 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 5
Kraft Sheleg O, Zaffryar Eilot S, Genin O, Yaseen W, Soueid Baumgarten S, Kessler O, et al. Localized LoxL3-Dependent Fibronectin Oxidation Regulates Myofiber Stretch and Integrin-Mediated Adhesion. Dev Cell. 2016;36:550-61 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
Xue Q, Varady S, Waddell T, Roman M, CARRINGTON J, Roh Johnson M. Lack of Paxillin phosphorylation promotes single-cell migration in vivo. J Cell Biol. 2023;222: pubmed publisher
Lopez Mejia I, Pijuan J, Navaridas R, Santacana M, Gatius S, Velasco A, et al. Oxidative stress-induced FAK activation contributes to uterine serous carcinoma aggressiveness. Mol Oncol. 2023;17:98-118 pubmed publisher
Bousgouni V, Inge O, Robertson D, Jones I, Clatworthy I, Bakal C. ARHGEF9 regulates melanoma morphogenesis in environments with diverse geometry and elasticity by promoting filopodial-driven adhesion. iScience. 2022;25:104795 pubmed publisher
Bachmann M, Skripka A, Weißenbruch K, Wehrle Haller B, Bastmeyer M. Phosphorylated paxillin and phosphorylated FAK constitute subregions within focal adhesions. J Cell Sci. 2022;135: pubmed publisher
Guo Y, Mei F, Huang Y, Ma S, Wei Y, Zhang X, et al. Matrix stiffness modulates tip cell formation through the p-PXN-Rac1-YAP signaling axis. Bioact Mater. 2022;7:364-376 pubmed publisher
Tilve S, Iweka C, Bao J, Hawken N, Mencio C, Geller H. Phospholipid phosphatase related 1 (PLPPR1) increases cell adhesion through modulation of Rac1 activity. Exp Cell Res. 2020;389:111911 pubmed publisher
Lu J, Doyle A, Shinsato Y, Wang S, Bodendorfer M, Zheng M, et al. Basement Membrane Regulates Fibronectin Organization Using Sliding Focal Adhesions Driven by a Contractile Winch. Dev Cell. 2020;52:631-646.e4 pubmed publisher
Verstappen J, Jin J, Kocer G, Haroon M, Jonkheijm P, Bakker A, et al. RGD-functionalized supported lipid bilayers modulate pre-osteoblast adherence and promote osteogenic differentiation. J Biomed Mater Res A. 2020;108:923-937 pubmed publisher
Jaumouillé V, Cartagena Rivera A, Waterman C. Coupling of β2 integrins to actin by a mechanosensitive molecular clutch drives complement receptor-mediated phagocytosis. Nat Cell Biol. 2019;21:1357-1369 pubmed publisher
Rezey A, Gerlach B, Wang R, Liao G, Tang D. Plk1 Mediates Paxillin Phosphorylation (Ser-272), Centrosome Maturation, and Airway Smooth Muscle Layer Thickening in Allergic Asthma. Sci Rep. 2019;9:7555 pubmed publisher
Rahikainen R, Ohman T, Turkki P, Varjosalo M, Hytönen V. Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling. J Cell Sci. 2019;: pubmed publisher
Iwasawa T, Zhang P, Ohkawa Y, Momota H, Wakabayashi T, Ohmi Y, et al. Enhancement of malignant properties of human glioma cells by ganglioside GD3/GD2. Int J Oncol. 2018;52:1255-1266 pubmed publisher
Yang X, Mei S, Niu H, Li J. Nicotinic acid impairs assembly of leading edge in glioma cells. Oncol Rep. 2017;38:829-836 pubmed publisher
Wu J, Chen Y, Kuo C, Wenshin Yu H, Chen Y, Chiou A, et al. Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration. Sci Rep. 2015;5:18476 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-Paxillin (Tyr31) Polyclonal Antibody
Catalog # :
44-720G
Quantity :
100 µL
Price :
US 514
Clonality :
Polyclonal
Purity :
Affinity chromatography
Host :
Rabbit
Reactivity :
Human, Mouse
Applications :
Immunocytochemistry: 1:250, Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000
Species :
Human, Mouse
Isotype :
IgG
Storage :
-20°C
Description :
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
Immunogen :
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 31.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:250, Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000
Aliases :
adaptor protein; AW108311; AW123232; FLJ16691; hCG_1778014; I79_001229; myocardial ischemic preconditioning associated protein 7; Pax; PAX-1; PAXI; Paxillin; paxillin a; paxillin alpha; pxn; pxna; testicular tissue protein Li 134; TGFB1I1; transforming growth factor beta 1 induced transcript 1; wu:fw71f12
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA