Phospho-PKR (Thr451) Polyclonal Antibody | Invitrogen 44-668G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-PKR (Thr451) Polyclonal Antibody

catalog :

44-668G

quantity :

100 µL

price :

US 446.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; rat; fig 3	Hong M, Nam K, Kim K, Kim S, Kim I. The small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives regulate global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha. Cell Stress Chaperones. 2016;21:485-97 pubmed publisher
	Hoff F, van Dijk A, Qiu Y, Hu C, Ries R, Ligeralde A, et al. Clinical relevance of proteomic profiling in de novo pediatric acute myeloid leukemia: a Children's Oncology Group study. Haematologica. 2022;107:2329-2343 pubmed publisher
	Watanabe T, Ninomiya H, Saitou T, Takanezawa S, Yamamoto S, Imai Y, et al. Therapeutic effects of the PKR inhibitor C16 suppressing tumor proliferation and angiogenesis in hepatocellular carcinoma in vitro and in vivo. Sci Rep. 2020;10:5133 pubmed publisher
	Nguyen T, Kabotyanski E, Reineke L, Shao J, Xiong F, Lee J, et al. The SINEB1 element in the long non-coding RNA Malat1 is necessary for TDP-43 proteostasis. Nucleic Acids Res. 2020;48:2621-2642 pubmed publisher
	Imai Y, Yoshida O, Watanabe T, Yukimoto A, Koizumi Y, Ikeda Y, et al. Stimulated hepatic stellate cell promotes progression of hepatocellular carcinoma due to protein kinase R activation. PLoS ONE. 2019;14:e0212589 pubmed publisher
	Hwang K, Bak M, Kim S, Rhee S, Lee Y. Restoring synaptic plasticity and memory in mouse models of Alzheimer's disease by PKR inhibition. Mol Brain. 2017;10:57 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-PKR (Thr451) Polyclonal Antibody

Catalog # :

44-668G

Quantity :

100 µL

Price :

US 446.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunohistochemistry: Assay-Dependent, Western Blot: 1:1,000

Species :

Human, Mouse

Isotype :

IgG

Storage :

-20°C

Description :

EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses. EIF2AK2 plays a key role in antiviral defense mediated through its direct activation by double-stranded RNA produced by viral infection. PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

Immunogen :

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PKR that contains threonine 451.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry: Assay-Dependent, Western Blot: 1:1,000

Aliases :

2310047A08Rik; 4732414G15Rik; AI467567; AI747578; double stranded RNA activated protein kinase; dsRNA-activated kinase; EC 2.7.11.1; eIF-2 alpha; eIF2a Kinase; eIF-2A protein kinase 2; EIF2AK1; EIF2AK2; eukaryotic translation initiation factor 2 alpha kinase 2; eukaryotic translation initiation factor 2-alpha kinase 2; IFN- type I-induced and dsRNA-activated kinase; IFN-induced and double-stranded RNA-activated kinase; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; interferon-inducible RNA-dependent protein kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PKR; PPP1R83; Prkr; protein kinase R; protein kinase RNA-activated; protein kinase, interferon inducible double stranded RNA dependent; Protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; serine/threonine-protein kinase TIK; T-cell viral integration site; Tik; Tyrosine-protein kinase EIF2AK2

more info or order :

Invitrogen product webpage