Phospho-FAK (Tyr407) Polyclonal Antibody | Invitrogen 44-650G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-FAK (Tyr407) Polyclonal Antibody

catalog :

44-650G

quantity :

100 µL

price :

US 450.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:50; fig 1 immunocytochemistry; human; 1:100; fig 2 western blot; human; 1:1000; fig 1	Su L, Li X, Wu X, Hui B, Han S, Gao J, et al. Simultaneous deactivation of FAK and Src improves the pathology of hypertrophic scar. Sci Rep. 2016;6:26023 pubmed publisher
immunohistochemistry - frozen section; rat; 1:100; fig 5	Tang E, Lee W, Cheng C. Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis. Endocrinology. 2016;157:1644-59 pubmed publisher
immunohistochemistry - frozen section; rat; 1:100; tbl 2	Li N, Mruk D, Mok K, Li M, Wong C, Lee W, et al. Connexin 43 reboots meiosis and reseals blood-testis barrier following toxicant-mediated aspermatogenesis and barrier disruption. FASEB J. 2016;30:1436-52 pubmed publisher
	Benwell C, Taylor J, Robinson S. Endothelial neuropilin-2 influences angiogenesis by regulating actin pattern development and α5-integrin-p-FAK complex recruitment to assembling adhesion sites. FASEB J. 2021;35:e21679 pubmed publisher
	Gao Y, Chen H, Xiao X, Lui W, Lee W, Mruk D, et al. Perfluorooctanesulfonate (PFOS)-induced Sertoli cell injury through a disruption of F-actin and microtubule organization is mediated by Akt1/2. Sci Rep. 2017;7:1110 pubmed publisher
	Robertson J, Jacquemet G, Byron A, Jones M, Warwood S, Selley J, et al. Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat Commun. 2015;6:6265 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-FAK (Tyr407) Polyclonal Antibody

Catalog # :

44-650G

Quantity :

100 µL

Price :

US 450.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Chicken, Human, Mouse, Rat

Applications :

Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000

Species :

Chicken, Human, Mouse, Rat

Isotype :

IgG

Storage :

-20°C

Description :

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

Immunogen :

Synthetic phosphopeptide from human FAK containing tyrosine 407. The sequence is conserved in mouse, rat and chicken.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000

Aliases :

CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK

more info or order :

Invitrogen product webpage