product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-FAK (Tyr861) Polyclonal Antibody
catalog :
44-626G
quantity :
100 µL
price :
US 446
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, chicken, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 29
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 6d
Reed M, Luissint A, Azcutia V, Fan S, O Leary M, Quirós M, et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo. Nat Commun. 2019;10:5004 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 8f
Li N, Xue W, Yuan H, Dong B, Ding Y, Liu Y, et al. AKT-mediated stabilization of histone methyltransferase WHSC1 promotes prostate cancer metastasis. J Clin Invest. 2017;127:1284-1302 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3c
Benito Jardón M, Klapproth S, Gimeno LLuch I, Petzold T, Bharadwaj M, Müller D, et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. elife. 2017;6: pubmed publisher
  • immunohistochemistry; chicken; 1:200; fig 7a
McKinney M, McLennan R, Kulesa P. Angiopoietin 2 signaling plays a critical role in neural crest cell migration. BMC Biol. 2016;14:111 pubmed
  • immunohistochemistry; mouse; fig 6a
  • western blot; mouse; fig 6e
Peng D, Ungewiss C, Tong P, Byers L, Wang J, Canales J, et al. ZEB1 induces LOXL2-mediated collagen stabilization and deposition in the extracellular matrix to drive lung cancer invasion and metastasis. Oncogene. 2017;36:1925-1938 pubmed publisher
  • western blot; human; fig 6
Adam A, Lowery A, Martino N, Alsaffar H, Vincent P. Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-α: Crosstalk with p38 Signaling. PLoS ONE. 2016;11:e0161975 pubmed publisher
  • immunocytochemistry; zebrafish ; tbl 4
Venero Galanternik M, Navajas Acedo J, Romero Carvajal A, Piotrowski T. Imaging collective cell migration and hair cell regeneration in the sensory lateral line. Methods Cell Biol. 2016;134:211-56 pubmed publisher
  • immunocytochemistry; mouse; 1:50
  • western blot; mouse; fig 5
Ungewiss C, Rizvi Z, Roybal J, Peng D, Gold K, Shin D, et al. The microRNA-200/Zeb1 axis regulates ECM-dependent β1-integrin/FAK signaling, cancer cell invasion and metastasis through CRKL. Sci Rep. 2016;6:18652 pubmed publisher
  • western blot; human; 1:1000; tbl 1
Oudart J, Doué M, Vautrin A, Brassart B, Sellier C, Dupont Deshorgue A, et al. The anti-tumor NC1 domain of collagen XIX inhibits the FAK/ PI3K/Akt/mTOR signaling pathway through αvβ3 integrin interaction. Oncotarget. 2016;7:1516-28 pubmed publisher
Rose M, Espinoza V, Hoff K, Pike L, Sharma V, Hofmann M, et al. BCL2L11 Induction Mediates Sensitivity to Src and MEK1/2 Inhibition in Thyroid Cancer. Cancers (Basel). 2023;15: pubmed publisher
Moustogiannis A, Philippou A, Zevolis E, Taso O, Giannopoulos A, Chatzigeorgiou A, et al. Effect of Mechanical Loading of Senescent Myoblasts on Their Myogenic Lineage Progression and Survival. Cells. 2022;11: pubmed publisher
Betriu N, Andreeva A, Alonso A, Semino C. Increased Stiffness Downregulates Focal Adhesion Kinase Expression in Pancreatic Cancer Cells Cultured in 3D Self-Assembling Peptide Scaffolds. Biomedicines. 2022;10: pubmed publisher
Si H, Zhao N, Pedroza A, Zaske A, Rosen J, Creighton C, et al. Noncanonical Wnt/Ror2 signaling regulates cell-matrix adhesion to prompt directional tumor cell invasion in breast cancer. Mol Biol Cell. 2022;33:ar103 pubmed publisher
Mahabaleshwar H, AshaRani P, Loo T, Koh S, Pitman M, Kwok S, et al. Slit-Robo signalling establishes a Sphingosine-1-phosphate gradient to polarise fin mesenchyme. EMBO Rep. 2022;23:e54464 pubmed publisher
Steenkiste E, Berndt J, Pilling C, Simpkins C, Cooper J. A Cas-BCAR3 co-regulatory circuit controls lamellipodia dynamics. elife. 2021;10: pubmed publisher
Lees D, Reynolds L, Pedrosa A, Roy Luzarraga M, Hodivala Dilke K. Phosphorylation of pericyte FAK-Y861 affects tumour cell apoptosis and tumour blood vessel regression. Angiogenesis. 2021;24:471-482 pubmed publisher
Yoon J, Wang J, Roehrl M. A combined FAK, c-MET, and MST1R three-protein panel risk-stratifies colorectal cancer patients. Transl Oncol. 2020;13:100836 pubmed publisher
Goldstein L, Perez R, Yardley D, Han L, Reuben J, Gao H, et al. A window-of-opportunity trial of the CXCR1/2 inhibitor reparixin in operable HER-2-negative breast cancer. Breast Cancer Res. 2020;22:4 pubmed publisher
McLennan R, McKinney M, Teddy J, Morrison J, Kasemeier Kulesa J, Ridenour D, et al. Neural crest cells bulldoze through the microenvironment using Aquaporin 1 to stabilize filopodia. Development. 2020;147: pubmed publisher
Hermida Prado F, Villaronga M, Granda Díaz R, Del Río Ibisate N, Santos L, Hermosilla M, et al. The SRC Inhibitor Dasatinib Induces Stem Cell-Like Properties in Head and Neck Cancer Cells that are Effectively Counteracted by the Mithralog EC-8042. J Clin Med. 2019;8: pubmed publisher
Wang B, Pan J, Yu H, Xiong L, Zhao K, Xiong S, et al. Lack of Myosin X Enhances Osteoclastogenesis and Increases Cell Surface Unc5b in Osteoclast-Lineage Cells. J Bone Miner Res. 2019;34:939-954 pubmed publisher
Kessler B, Mishall K, Kellett M, Clark E, Pugazhenthi U, Pozdeyev N, et al. Resistance to Src inhibition alters the BRAF-mutant tumor secretome to promote an invasive phenotype and therapeutic escape through a FAK>p130Cas>c-Jun signaling axis. Oncogene. 2019;38:2565-2579 pubmed publisher
Hughes V, Siemann D. Treatment with Src inhibitor Dasatinib results in elevated metastatic potential in the 4T1 murine mammary carcinoma model. Tumor Microenviron. 2018;1:30-36 pubmed publisher
Miskolczi Z, Smith M, Rowling E, Ferguson J, Barriuso J, Wellbrock C. Collagen abundance controls melanoma phenotypes through lineage-specific microenvironment sensing. Oncogene. 2018;37:3166-3182 pubmed publisher
Beadnell T, Nassar K, Rose M, Clark E, Danysh B, Hofmann M, et al. Src-mediated regulation of the PI3K pathway in advanced papillary and anaplastic thyroid cancer. Oncogenesis. 2018;7:23 pubmed publisher
Mishall K, Beadnell T, Kuenzi B, Klimczak D, Superti Furga G, Rix U, et al. Sustained activation of the AKT/mTOR and MAP kinase pathways mediate resistance to the Src inhibitor, dasatinib, in thyroid cancer. Oncotarget. 2017;8:103014-103031 pubmed publisher
Moen I, Gebre M, Alonso Camino V, Chen D, Epstein D, McDonald D. Anti-metastatic action of FAK inhibitor OXA-11 in combination with VEGFR-2 signaling blockade in pancreatic neuroendocrine tumors. Clin Exp Metastasis. 2015;32:799-817 pubmed publisher
Vecino E, Heller J, Veiga Crespo P, Martin K, Fawcett J. Influence of extracellular matrix components on the expression of integrins and regeneration of adult retinal ganglion cells. PLoS ONE. 2015;10:e0125250 pubmed publisher
Rios Doria J, Sabol D, Chesebrough J, Stewart D, Xu L, Tammali R, et al. A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways. Mol Cancer Ther. 2015;14:1637-49 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-FAK (Tyr861) Polyclonal Antibody
Catalog # :
44-626G
Quantity :
100 µL
Price :
US 446
Clonality :
Polyclonal
Purity :
Affinity chromatography
Host :
Rabbit
Reactivity :
Chicken, Human, Mouse
Applications :
Immunocytochemistry: 1:250, Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000
Species :
Chicken, Human, Mouse
Isotype :
IgG
Storage :
-20°C
Description :
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
Immunogen :
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 861. The sequence is conserved in mouse, rat, chicken and frog.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:250, Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000
Aliases :
CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA