product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-FAK (Tyr397) Polyclonal Antibody
catalog :
44-624G
quantity :
100 µL
price :
US 460
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, rat, chicken, zebrafish , fruit fly , Xenopus laevis, purple urchin
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
citations: 100
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:500; fig s1c
Ozmadenci D, Shankara Narayanan J, Andrew J, Ojalill M, Barrie A, Jiang S, et al. Tumor FAK orchestrates immunosuppression in ovarian cancer via the CD155/TIGIT axis. Proc Natl Acad Sci U S A. 2022;119:e2117065119 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1g
Takahashi K, Kanerva K, Vanharanta L, Almeida Souza L, Lietha D, Olkkonen V, et al. ORP2 couples LDL-cholesterol transport to FAK activation by endosomal cholesterol/PI(4,5)P2 exchange. EMBO J. 2021;40:e106871 pubmed publisher
  • western blot; mouse; loading ...; fig 1c
Errico A, Stocco A, Riccardi V, Gambalunga A, Bassetto F, Grigatti M, et al. Neurofibromin Deficiency and Extracellular Matrix Cooperate to Increase Transforming Potential through FAK-Dependent Signaling. Cancers (Basel). 2021;13: pubmed publisher
  • immunocytochemistry; rat; 1:50; loading ...; fig 2g
  • western blot; rat; loading ...; fig 2i
Cao F, Zhou Y, Liu X, Yu C. Podosome formation promotes plasma membrane invagination and integrin-β3 endocytosis on a viscous RGD-membrane. Commun Biol. 2020;3:117 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig s7
Reed M, Luissint A, Azcutia V, Fan S, O Leary M, Quirós M, et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo. Nat Commun. 2019;10:5004 pubmed publisher
  • immunohistochemistry; fruit fly ; loading ...; fig 1a
Chen X, Wanggou S, Bodalia A, Zhu M, Dong W, Fan J, et al. A Feedforward Mechanism Mediated by Mechanosensitive Ion Channel PIEZO1 and Tissue Mechanics Promotes Glioma Aggression. Neuron. 2018;100:799-815.e7 pubmed publisher
  • immunohistochemistry; zebrafish ; loading ...; fig s7c
Kara N, Wei C, Commanday A, Patton J. miR-27 regulates chondrogenesis by suppressing focal adhesion kinase during pharyngeal arch development. Dev Biol. 2017;429:321-334 pubmed publisher
  • western blot; human; 1:500; fig 3b
Wu Y, Jhao Y, Cheng Y, Chen Y. 15-Deoxy-?12,14-prostaglandin J2 inhibits migration of human thyroid carcinoma cells by disrupting focal adhesion complex and adherens junction. Oncol Lett. 2017;13:2569-2576 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 1b
Sato M, Kawana K, Adachi K, Fujimoto A, Yoshida M, Nakamura H, et al. Targeting glutamine metabolism and the focal adhesion kinase additively inhibits the mammalian target of the rapamycin pathway in spheroid cancer stem-like properties of ovarian clear cell carcinoma in vitro. Int J Oncol. 2017;50:1431-1438 pubmed publisher
  • immunocytochemistry; human; fig 4c
  • western blot; human; fig 4a
El Kharbili M, Robert C, Witkowski T, Danty Berger E, Barbollat Boutrand L, Masse I, et al. Tetraspanin 8 is a novel regulator of ILK-driven ?1 integrin adhesion and signaling in invasive melanoma cells. Oncotarget. 2017;8:17140-17155 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3c
Benito Jardón M, Klapproth S, Gimeno LLuch I, Petzold T, Bharadwaj M, Müller D, et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. elife. 2017;6: pubmed publisher
  • western blot; human; fig 6
Adam A, Lowery A, Martino N, Alsaffar H, Vincent P. Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-α: Crosstalk with p38 Signaling. PLoS ONE. 2016;11:e0161975 pubmed publisher
  • immunocytochemistry; rat; fig 1
Harizanova J, Fermin Y, Malik Sheriff R, Wieczorek J, Ickstadt K, Grecco H, et al. Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions. PLoS ONE. 2016;11:e0160591 pubmed publisher
  • western blot; human; loading ...; fig 4c
Barcus C, Keely P, Eliceiri K, Schuler L. Prolactin signaling through focal adhesion complexes is amplified by stiff extracellular matrices in breast cancer cells. Oncotarget. 2016;7:48093-48106 pubmed publisher
  • western blot; human; fig 2
Segatto I, Massarut S, Boyle R, Baldassarre G, Walker D, Belletti B. Preclinical validation of a novel compound targeting p70S6 kinase in breast cancer. Aging (Albany NY). 2016;8:958-76 pubmed publisher
  • immunocytochemistry; mouse; 1:150; fig 2
Elosegui Artola A, Oria R, Chen Y, Kosmalska A, Pérez González C, Castro N, et al. Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity. Nat Cell Biol. 2016;18:540-8 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2a
Nader G, Ezratty E, Gundersen G. FAK, talin and PIPKI? regulate endocytosed integrin activation to polarize focal adhesion assembly. Nat Cell Biol. 2016;18:491-503 pubmed publisher
  • immunocytochemistry; mouse; fig 5
Rooney N, Wang P, Brennan K, Gilmore A, Streuli C. The Integrin-Mediated ILK-Parvin-?Pix Signaling Axis Controls Differentiation in Mammary Epithelial Cells. J Cell Physiol. 2016;231:2408-17 pubmed publisher
  • immunocytochemistry; human; fig 5
Hayashi K, Michiue H, Yamada H, Takata K, Nakayama H, Wei F, et al. Fluvoxamine, an anti-depressant, inhibits human glioblastoma invasion by disrupting actin polymerization. Sci Rep. 2016;6:23372 pubmed publisher
  • immunocytochemistry; mouse; 1:400; fig 5s3
  • western blot; mouse; 1:1000; fig 5s3
Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
  • western blot; human; fig 7
Couderc C, Boin A, Fuhrmann L, Vincent Salomon A, Mandati V, Kieffer Y, et al. AMOTL1 Promotes Breast Cancer Progression and Is Antagonized by Merlin. Neoplasia. 2016;18:10-24 pubmed publisher
Estep J, Sun L, Riccomagno M. A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events. Heliyon. 2023;9:e15282 pubmed publisher
Delgado Bellido D, Zamudio Mart xed nez E, Fern xe1 ndez Cort xe9 s M, Herrera Campos A, Olmedo Pelayo J, Perez C, et al. VE-Cadherin modulates β-catenin/TCF-4 to enhance Vasculogenic Mimicry. Cell Death Dis. 2023;14:135 pubmed publisher
Kuhn C, Basnet N, Bodakuntla S, Alvarez Brecht P, Nichols S, Martinez Sanchez A, et al. Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 spike protein. Nat Commun. 2023;14:620 pubmed publisher
Hu Z, Wei F, Su Y, Wang Y, Shen Y, Fang Y, et al. Histone deacetylase inhibitors promote breast cancer metastasis by elevating NEDD9 expression. Signal Transduct Target Ther. 2023;8:11 pubmed publisher
Simsek M, Chandel A, Saparov D, Zinani O, Clason N, xd6 zbudak E. Periodic inhibition of Erk activity drives sequential somite segmentation. Nature. 2023;613:153-159 pubmed publisher
Khalil M, De Benedetti A. The TLK1-MK5 Axis Regulates Motility, Invasion, and Metastasis of Prostate Cancer Cells. Cancers (Basel). 2022;14: pubmed publisher
Ventura E, Xie C, Buraschi S, Belfiore A, Iozzo R, Giordano A, et al. Complexity of progranulin mechanisms of action in mesothelioma. J Exp Clin Cancer Res. 2022;41:333 pubmed publisher
Wisniewski D, Liyasova M, Korrapati S, Zhang X, Ratnayake S, Chen Q, et al. Flotillin-2 regulates epidermal growth factor receptor activation, degradation by Cbl-mediated ubiquitination, and cancer growth. J Biol Chem. 2022;299:102766 pubmed publisher
Kuhn C, Basnet N, Bodakuntla S, Alvarez Brecht P, Nichols S, Martinez Sanchez A, et al. Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein. bioRxiv. 2022;: pubmed publisher
Peng K, Li S, Li Q, Zhang C, Yuan Y, Liu M, et al. Positive Phospho-Focal Adhesion Kinase in Gastric Cancer Associates With Poor Prognosis After Curative Resection. Front Oncol. 2022;12:953938 pubmed publisher
Zhuo Y, Crecelius J, Marchese A. G protein-coupled receptor kinase phosphorylation of distal C-tail sites specifies βarrestin1-mediated signaling by chemokine receptor CXCR4. J Biol Chem. 2022;298:102351 pubmed publisher
Filhol O, Hesse A, Bouin A, Albig xe8 s Rizo C, Jeanneret F, Battail C, et al. CK2β Is a Gatekeeper of Focal Adhesions Regulating Cell Spreading. Front Mol Biosci. 2022;9:900947 pubmed publisher
Lee J, Ng H, Lin Y, Lin T, Kao C, Shie M. The Synergistic Effect of Cyclic Tensile Force and Periodontal Ligament Cell-Laden Calcium Silicate/Gelatin Methacrylate Auxetic Hydrogel Scaffolds for Bone Regeneration. Cells. 2022;11: pubmed publisher
Pasapera A, Heissler S, Eto M, Nishimura Y, Fischer R, Thiam H, et al. MARK2 regulates directed cell migration through modulation of myosin II contractility and focal adhesion organization. Curr Biol. 2022;32:2704-2718.e6 pubmed publisher
Ma R, Gong D, You H, Xu C, Lu Y, Bergers G, et al. LGL1 binds to Integrin β1 and inhibits downstream signaling to promote epithelial branching in the mammary gland. Cell Rep. 2022;38:110375 pubmed publisher
Al Hasani J, Sens Albert C, Ghosh S, Trogisch F, Nahar T, Friede P, et al. Zyxin protects from hypertension-induced cardiac dysfunction. Cell Mol Life Sci. 2022;79:93 pubmed publisher
Qi L, Knifley T, Chen M, O Connor K. Integrin α6β4 requires plectin and vimentin for adhesion complex distribution and invasive growth. J Cell Sci. 2022;135: pubmed publisher
Mehta S, Fiorelli R, Bao X, Pennington Krygier C, Derogatis A, Kim S, et al. A Phase 0 Trial of Ceritinib in Patients with Brain Metastases and Recurrent Glioblastoma. Clin Cancer Res. 2022;28:289-297 pubmed publisher
Guo Y, Mei F, Huang Y, Ma S, Wei Y, Zhang X, et al. Matrix stiffness modulates tip cell formation through the p-PXN-Rac1-YAP signaling axis. Bioact Mater. 2022;7:364-376 pubmed publisher
Mao M, Popli T, Jeanne M, Hoff K, Sen S, Gould D. Identification of fibronectin 1 as a candidate genetic modifier in a Col4a1 mutant mouse model of Gould syndrome. Dis Model Mech. 2021;14: pubmed publisher
Steenkiste E, Berndt J, Pilling C, Simpkins C, Cooper J. A Cas-BCAR3 co-regulatory circuit controls lamellipodia dynamics. elife. 2021;10: pubmed publisher
Reed D, Zhao Y, Han M, Mercuri L, Miloro M. Mechanical Loading Disrupts Focal Adhesion Kinase Activation in Mandibular Fibrochondrocytes During Murine Temporomandibular Joint Osteoarthritis. J Oral Maxillofac Surg. 2021;79:2058.e1-2058.e15 pubmed publisher
Timbrell S, Aglan H, Cramer A, Foden P, Weaver D, Pachter J, et al. FAK inhibition alone or in combination with adjuvant therapies reduces cancer stem cell activity. NPJ Breast Cancer. 2021;7:65 pubmed publisher
Szulczewski J, Inman D, Proestaki M, Notbohm J, Burkel B, Ponik S. Directional cues in the tumor microenvironment due to cell contraction against aligned collagen fibers. Acta Biomater. 2021;129:96-109 pubmed publisher
Lees D, Reynolds L, Pedrosa A, Roy Luzarraga M, Hodivala Dilke K. Phosphorylation of pericyte FAK-Y861 affects tumour cell apoptosis and tumour blood vessel regression. Angiogenesis. 2021;24:471-482 pubmed publisher
Wei Z, Schnellmann R, Pruitt H, Gerecht S. Hydrogel Network Dynamics Regulate Vascular Morphogenesis. Cell Stem Cell. 2020;27:798-812.e6 pubmed publisher
Hisamatsu E, Nagao M, Toh R, Irino Y, Iino T, Hara T, et al. Fibronectin-containing High-Density Lipoprotein is Associated with Cancer Cell Adhesion and Proliferation. Kobe J Med Sci. 2020;66:E40-E48 pubmed
Ramovs V, Krotenberg Garcia A, Kreft M, Sonnenberg A. Integrin α3β1 Is a Key Regulator of Several Protumorigenic Pathways during Skin Carcinogenesis. J Invest Dermatol. 2020;: pubmed publisher
Zhuo Y, Gurevich V, Vishnivetskiy S, Klug C, Marchese A. A non-GPCR-binding partner interacts with a novel surface on β-arrestin1 to mediate GPCR signaling. J Biol Chem. 2020;295:14111-14124 pubmed publisher
Ramirez Ricardo J, Leal Orta E, Martínez Baeza E, Ortiz Mendoza C, Breton Mora F, Herrera Torres A, et al. Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src‑dependent pathway in MDA‑MB‑231 breast cancer cells. Mol Med Rep. 2020;22:1932-1948 pubmed publisher
Su S, Begum S, Ezratty E. An IFT20 mechano-trafficking axis is required for integrin recycling, focal adhesion dynamics and polarized cell migration. Mol Biol Cell. 2020;:mbcE20040232 pubmed publisher
Kim S, Jang S, Kim H, Chung S, Park J, Kuh H. Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model. Cancers (Basel). 2020;12: pubmed publisher
Franco Barraza J, Raghavan K, Luong T, Cukierman E. Engineering clinically-relevant human fibroblastic cell-derived extracellular matrices. Methods Cell Biol. 2020;156:109-160 pubmed publisher
Randles M, Lausecker F, Humphries J, Byron A, Clark S, Miner J, et al. Basement membrane ligands initiate distinct signalling networks to direct cell shape. Matrix Biol. 2020;90:61-78 pubmed publisher
Zhou X, Wahane S, Friedl M, Kluge M, Friedel C, Avrampou K, et al. Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2. Nat Neurosci. 2020;23:337-350 pubmed publisher
Lu J, Doyle A, Shinsato Y, Wang S, Bodendorfer M, Zheng M, et al. Basement Membrane Regulates Fibronectin Organization Using Sliding Focal Adhesions Driven by a Contractile Winch. Dev Cell. 2020;52:631-646.e4 pubmed publisher
Peterman E, Gibieža P, Schafer J, Skeberdis V, Kaupinis A, Valius M, et al. The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation. Nat Commun. 2019;10:3181 pubmed publisher
Schreiber C, Saraswati S, Harkins S, Gruber A, Cremers N, Thiele W, et al. Loss of ASAP1 in mice impairs adipogenic and osteogenic differentiation of mesenchymal progenitor cells through dysregulation of FAK/Src and AKT signaling. PLoS Genet. 2019;15:e1008216 pubmed publisher
Cordes N, Ney M, Beleites T, Aust D, Baretton G, Thames H, et al. Retrospective investigation of the prognostic value of the β1 integrin expression in patients with head and neck squamous cell carcinoma receiving primary radio(chemo)therapy. PLoS ONE. 2018;13:e0209479 pubmed publisher
Gemperle J, Dibus M, Koudelková L, Rosel D, Brabek J. The interaction of p130Cas with PKN3 promotes malignant growth. Mol Oncol. 2019;13:264-289 pubmed publisher
Ruoss S, Kindt P, Oberholzer L, Rohner M, Jungck L, Abdel Aziz S, et al. Inhibition of calpain delays early muscle atrophy after rotator cuff tendon release in sheep. Physiol Rep. 2018;6:e13833 pubmed publisher
Tiede S, Meyer Schaller N, Kalathur R, Ivanek R, Fagiani E, Schmassmann P, et al. The FAK inhibitor BI 853520 exerts anti-tumor effects in breast cancer. Oncogenesis. 2018;7:73 pubmed publisher
Wang W, Lollis E, Bordeleau F, Reinhart King C. Matrix stiffness regulates vascular integrity through focal adhesion kinase activity. FASEB J. 2019;33:1199-1208 pubmed publisher
de Jong S, Crequer A, Matos I, Hum D, Gunasekharan V, Lorenzo L, et al. The human CIB1-EVER1-EVER2 complex governs keratinocyte-intrinsic immunity to β-papillomaviruses. J Exp Med. 2018;215:2289-2310 pubmed publisher
Heim J, McDonald C, Wyles S, Sominidi Damodaran S, Squirewell E, Li M, et al. FAK auto-phosphorylation site tyrosine 397 is required for development but dispensable for normal skin homeostasis. PLoS ONE. 2018;13:e0200558 pubmed publisher
Sato J, Horibe S, Kawauchi S, Sasaki N, Hirata K, Rikitake Y. Involvement of aquaporin-4 in laminin-enhanced process formation of mouse astrocytes in 2D culture: Roles of dystroglycan and α-syntrophin in aquaporin-4 expression. J Neurochem. 2018;147:495-513 pubmed publisher
Wan J, Zhang J, Zhang J. Expression of p53 and its mechanism in prostate cancer. Oncol Lett. 2018;16:378-382 pubmed publisher
Jamalpour M, Li X, Gustafsson K, Tyner J, Welsh M. Disparate effects of Shb gene deficiency on disease characteristics in murine models of myeloid, B-cell, and T-cell leukemia. Tumour Biol. 2018;40:1010428318771472 pubmed publisher
Bastounis E, Yeh Y, Theriot J. Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin. Mol Biol Cell. 2018;29:1571-1589 pubmed publisher
Villaronga M, Hermida Prado F, Granda Díaz R, Menendez S, Álvarez Teijeiro S, Quer M, et al. Immunohistochemical Expression of Cortactin and Focal Adhesion Kinase Predicts Recurrence Risk and Laryngeal Cancer Risk Beyond Histologic Grading. Cancer Epidemiol Biomarkers Prev. 2018;27:805-813 pubmed publisher
Pincha N, Hajam E, Badarinath K, Batta S, Masudi T, Dey R, et al. PAI1 mediates fibroblast-mast cell interactions in skin fibrosis. J Clin Invest. 2018;128:1807-1819 pubmed publisher
Nakagawa H, Sasagawa S, Itoh K. Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells. Oncol Lett. 2018;15:1495-1502 pubmed publisher
Chen P, Muzumdar M, Dorans K, Robbins R, Bhutkar A, Del Rosario A, et al. Adaptive and Reversible Resistance to Kras Inhibition in Pancreatic Cancer Cells. Cancer Res. 2018;78:985-1002 pubmed publisher
Nakatsuka E, Sawada K, Nakamura K, Yoshimura A, Kinose Y, Kodama M, et al. Plasminogen activator inhibitor-1 is an independent prognostic factor of ovarian cancer and IMD-4482, a novel plasminogen activator inhibitor-1 inhibitor, inhibits ovarian cancer peritoneal dissemination. Oncotarget. 2017;8:89887-89902 pubmed publisher
Elosegui Artola A, Andreu I, Beedle A, Lezamiz A, Uroz M, Kosmalska A, et al. Force Triggers YAP Nuclear Entry by Regulating Transport across Nuclear Pores. Cell. 2017;171:1397-1410.e14 pubmed publisher
Kawano S, Torisu T, Esaki M, Torisu K, Matsuno Y, Kitazono T. Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion. Biol Open. 2017;6:1644-1653 pubmed publisher
Vo T, Park J, Seo J, Lee E, Choi H, Bae S, et al. ARD1-mediated aurora kinase A acetylation promotes cell proliferation and migration. Oncotarget. 2017;8:57216-57230 pubmed publisher
Ruoss S, Möhl C, Benn M, von Rechenberg B, Wieser K, Meyer D, et al. Costamere protein expression and tissue composition of rotator cuff muscle after tendon release in sheep. J Orthop Res. 2018;36:272-281 pubmed publisher
Frismantiene A, Kyriakakis E, Dasen B, Erne P, Resink T, Philippova M. Actin cytoskeleton regulates functional anchorage-migration switch during T-cadherin-induced phenotype modulation of vascular smooth muscle cells. Cell Adh Migr. 2018;12:69-85 pubmed publisher
Mon N, Senga T, Ito S. Interleukin-1? activates focal adhesion kinase and Src to induce matrix metalloproteinase-9 production and invasion of MCF-7 breast cancer cells. Oncol Lett. 2017;13:955-960 pubmed publisher
Heim J, Squirewell E, Neu A, Zocher G, Sominidi Damodaran S, Wyles S, et al. Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix. Proc Natl Acad Sci U S A. 2017;114:3933-3938 pubmed publisher
Love J, Bober B, Orozco E, White A, Bremner S, Lovering R, et al. mTOR regulates peripheral nerve response to tensile strain. J Neurophysiol. 2017;117:2075-2084 pubmed publisher
Fukumoto M, Ijuin T, Takenawa T. PI(3,4)P2 plays critical roles in the regulation of focal adhesion dynamics of MDA-MB-231 breast cancer cells. Cancer Sci. 2017;108:941-951 pubmed publisher
Hirai M, Arita Y, McGlade C, Lee K, Chen J, Evans S. Adaptor proteins NUMB and NUMBL promote cell cycle withdrawal by targeting ERBB2 for degradation. J Clin Invest. 2017;127:569-582 pubmed publisher
Cheng Z, Zhu Q, Dee R, Opheim Z, Mack C, Cyr D, et al. Focal Adhesion Kinase-mediated Phosphorylation of Beclin1 Protein Suppresses Cardiomyocyte Autophagy and Initiates Hypertrophic Growth. J Biol Chem. 2017;292:2065-2079 pubmed publisher
Thang N, Minh N, Huong P. Translocation of BBAP from the cytoplasm to the nucleus reduces the metastatic ability of vemurafenib-resistant SKMEL28 cells. Mol Med Rep. 2017;15:317-322 pubmed publisher
Krupke O, Zysk I, Mellott D, Burke R. Eph and Ephrin function in dispersal and epithelial insertion of pigmented immunocytes in sea urchin embryos. elife. 2016;5: pubmed publisher
Horton E, Humphries J, Stutchbury B, Jacquemet G, Ballestrem C, Barry S, et al. Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition. J Cell Biol. 2016;212:349-64 pubmed publisher
Austen K, Ringer P, Mehlich A, Chrostek Grashoff A, Kluger C, Klingner C, et al. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat Cell Biol. 2015;17:1597-606 pubmed publisher
Fiore V, Strane P, Bryksin A, White E, Hagood J, Barker T. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211:173-90 pubmed publisher
Chatani M, Mantoku A, Takeyama K, Abduweli D, Sugamori Y, Aoki K, et al. Microgravity promotes osteoclast activity in medaka fish reared at the international space station. Sci Rep. 2015;5:14172 pubmed publisher
Gjorevski N, Piotrowski A, Varner V, Nelson C. Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices. Sci Rep. 2015;5:11458 pubmed publisher
Castelli M, De Pascalis C, Distefano G, Ducano N, Oldani A, Lanzetti L, et al. Regulation of the microtubular cytoskeleton by Polycystin-1 favors focal adhesions turnover to modulate cell adhesion and migration. BMC Cell Biol. 2015;16:15 pubmed publisher
Fukumoto M, Kurisu S, Yamada T, Takenawa T. α-Actinin-4 enhances colorectal cancer cell invasion by suppressing focal adhesion maturation. PLoS ONE. 2015;10:e0120616 pubmed publisher
Curran C, Carrillo E, Ponik S, Keely P. Collagen density regulates xenobiotic and hypoxic response of mammary epithelial cells. Environ Toxicol Pharmacol. 2015;39:114-24 pubmed publisher
Green C, Fraser S, Day M. Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro. Hum Reprod. 2015;30:284-98 pubmed publisher
LeGrand K, Matsumoto H, Young G. A novel type 3 secretion system effector, YspI of Yersinia enterocolitica, induces cell paralysis by reducing total focal adhesion kinase. Cell Microbiol. 2015;17:688-701 pubmed publisher
Israeli Rosenberg S, Chen C, Li R, Deussen D, Niesman I, Okada H, et al. Caveolin modulates integrin function and mechanical activation in the cardiomyocyte. FASEB J. 2015;29:374-84 pubmed publisher
Stewart J, Ma X, Megison M, Nabers H, Cance W, Kurenova E, et al. Inhibition of FAK and VEGFR-3 binding decreases tumorigenicity in neuroblastoma. Mol Carcinog. 2015;54:9-23 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-FAK (Tyr397) Polyclonal Antibody
Catalog # :
44-624G
Quantity :
100 µL
Price :
US 460
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Chicken, Drosophila, Human, Mouse, Xenopus laevis
Applications :
Immunocytochemistry: Assay-dependent, Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000
Species :
Chicken, Drosophila, Human, Mouse, Xenopus laevis
Isotype :
IgG
Storage :
-20°C
Description :
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
Immunogen :
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: Assay-dependent, Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000
Aliases :
CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA