product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-AKT1 (Ser473) Monoclonal Antibody (14-6)
catalog :
44-621G
quantity :
100 µL
price :
US 514
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
14-6
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 66
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 6d
Sun Y, Jing J, Xu H, Xu L, Hu H, Tang C, et al. N-cadherin inhibitor creates a microenvironment that protect TILs from immune checkpoints and Treg cells. J Immunother Cancer. 2021;9: pubmed publisher
  • western blot; human; fig 5b
Matte I, Garde Granger P, Bessette P, Piche A. Ascites from ovarian cancer patients stimulates MUC16 mucin expression and secretion in human peritoneal mesothelial cells through an Akt-dependent pathway. BMC Cancer. 2019;19:406 pubmed publisher
  • western blot; mouse; loading ...; fig s3a
He P, Yang J, Yang V, Bialkowska A. Krüppel-like Factor 5, Increased in Pancreatic Ductal Adenocarcinoma, Promotes Proliferation, Acinar-to-Ductal Metaplasia, Pancreatic Intraepithelial Neoplasia, and Tumor Growth in Mice. Gastroenterology. 2018;154:1494-1508.e13 pubmed publisher
  • western blot; mouse; loading ...; fig 2c
Chamma C, Bargut T, Mandarim de Lacerda C, Aguila M. A rich medium-chain triacylglycerol diet benefits adiposity but has adverse effects on the markers of hepatic lipogenesis and beta-oxidation. Food Funct. 2017;8:778-787 pubmed publisher
  • western blot; mouse; loading ...; fig 5d
Martins F, Bargut T, Aguila M, Mandarim de Lacerda C. Thermogenesis, fatty acid synthesis with oxidation, and inflammation in the brown adipose tissue of ob/ob (-/-) mice. Ann Anat. 2017;210:44-51 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 4g
Graus Nunes F, Marinho T, Barbosa da Silva S, Aguila M, Mandarim de Lacerda C, Souza Mello V. Differential effects of angiotensin receptor blockers on pancreatic islet remodelling and glucose homeostasis in diet-induced obese mice. Mol Cell Endocrinol. 2017;439:54-64 pubmed publisher
  • western blot; rat; 1:2500; fig 6
Puchert M, Adams V, Linke A, Engele J. Evidence for the involvement of the CXCL12 system in the adaptation of skeletal muscles to physical exercise. Cell Signal. 2016;28:1205-15 pubmed publisher
  • western blot; human; 1:1000; fig 3
Elisia I, Nakamura H, Lam V, Hofs E, Cederberg R, Cait J, et al. DMSO Represses Inflammatory Cytokine Production from Human Blood Cells and Reduces Autoimmune Arthritis. PLoS ONE. 2016;11:e0152538 pubmed publisher
  • western blot; mouse; 1:500
Borges C, Salles A, Bringhenti I, Souza Mello V, Mandarim de Lacerda C, Aguila M. Adverse effects of vitamin D deficiency on the Pi3k/Akt pathway and pancreatic islet morphology in diet-induced obese mice. Mol Nutr Food Res. 2016;60:346-57 pubmed publisher
  • western blot; mouse; 1:1000; fig 6
Rueda Rincon N, Bloch K, Derua R, Vyas R, Harms A, Hankemeier T, et al. p53 attenuates AKT signaling by modulating membrane phospholipid composition. Oncotarget. 2015;6:21240-54 pubmed
  • western blot; mouse
Bargut T, Mandarim de Lacerda C, Aguila M. A high-fish-oil diet prevents adiposity and modulates white adipose tissue inflammation pathways in mice. J Nutr Biochem. 2015;26:960-9 pubmed publisher
  • western blot; human; fig 5a
Matte I, Lane D, Laplante C, Garde Granger P, Rancourt C, Piché A. Ovarian cancer ascites enhance the migration of patient-derived peritoneal mesothelial cells via cMet pathway through HGF-dependent and -independent mechanisms. Int J Cancer. 2015;137:289-98 pubmed publisher
  • western blot; human
Beltran P, Calzone F, Mitchell P, Chung Y, Cajulis E, Moody G, et al. Ganitumab (AMG 479) inhibits IGF-II-dependent ovarian cancer growth and potentiates platinum-based chemotherapy. Clin Cancer Res. 2014;20:2947-58 pubmed publisher
  • western blot; human; fig 3
Diaz Flores E, Goldschmidt H, Depeille P, Ng V, Akutagawa J, Krisman K, et al. PLC-? and PI3K link cytokines to ERK activation in hematopoietic cells with normal and oncogenic Kras. Sci Signal. 2013;6:ra105 pubmed publisher
  • western blot; human; fig 4
Lee H, Schaefer G, Heffron T, Shao L, Ye X, Sideris S, et al. Noncovalent wild-type-sparing inhibitors of EGFR T790M. Cancer Discov. 2013;3:168-81 pubmed publisher
  • western blot; mouse; fig 7
Poovassery J, Bishop G. Type I IFN receptor and the B cell antigen receptor regulate TLR7 responses via distinct molecular mechanisms. J Immunol. 2012;189:1757-64 pubmed publisher
  • western blot; human; fig 2
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
  • western blot; mouse; fig 7
Ruschmann J, Antignano F, Lam V, Snyder K, Kim C, Essak M, et al. The role of SHIP in the development and activation of mouse mucosal and connective tissue mast cells. J Immunol. 2012;188:3839-50 pubmed publisher
  • western blot; rat; fig 4
Shohat M, Ben Meir D, Lavi S. Protein phosphatase magnesium dependent 1A (PPM1A) plays a role in the differentiation and survival processes of nerve cells. PLoS ONE. 2012;7:e32438 pubmed publisher
  • western blot; human; fig 2
Wilson T, Lee D, Berry L, Shames D, Settleman J. Neuregulin-1-mediated autocrine signaling underlies sensitivity to HER2 kinase inhibitors in a subset of human cancers. Cancer Cell. 2011;20:158-72 pubmed publisher
  • western blot; mouse; fig 6
Cerezo A, Guadamillas M, Goetz J, Sánchez Perales S, Klein E, Assoian R, et al. The absence of caveolin-1 increases proliferation and anchorage- independent growth by a Rac-dependent, Erk-independent mechanism. Mol Cell Biol. 2009;29:5046-59 pubmed publisher
Vav xe1 kov xe1 M, Hofwimmer K, Laurencikiene J, G xf6 ransson O. Mechanism of TNFα-induced downregulation of salt-inducible kinase 2 in adipocytes. Sci Rep. 2023;13:10559 pubmed publisher
Jolly G, Duka T, Shivapurkar N, Chen W, Bansal S, Cheema A, et al. Cholecystokinin Receptor Antagonist Induces Pancreatic Stellate Cell Plasticity Rendering the Tumor Microenvironment Less Oncogenic. Cancers (Basel). 2023;15: pubmed publisher
Chen B, Zheng L, Zhu T, Jiao K. LncRNA FOXD3-AS1 aggravates myocardial ischemia/reperfusion injury by inactivating the Redd1/AKT/GSK3β/Nrf2 signaling pathway via the miR-128/TXNIP axis. J Biochem Mol Toxicol. 2022;36:e23218 pubmed publisher
Cabral Dias R, Lucarelli S, Zak K, Rahmani S, Judge G, Abousawan J, et al. Fyn and TOM1L1 are recruited to clathrin-coated pits and regulate Akt signaling. J Cell Biol. 2022;221: pubmed publisher
Jang S, Jung B, An Y, Jang H. The phosphatase and tensin homolog gene inserted between NP and P gene of recombinant New castle disease virus oncolytic effect test to glioblastoma cell and xenograft mouse model. Virol J. 2022;19:21 pubmed publisher
Fang C, Kang B, Zhao P, Ran J, Wang L, Zhao L, et al. Targeting Neuregulin 1 (NRG1): A Novel Biomarker for Non-Small-Cell Lung Cancer. J Environ Pathol Toxicol Oncol. 2021;40:61-72 pubmed publisher
Xiong S, Zhao Y, Xu T. DNA methyltransferase 3 beta mediates the methylation of the microRNA-34a promoter and enhances chondrocyte viability in osteoarthritis. Bioengineered. 2021;12:11138-11155 pubmed publisher
Ren S, Tan X, Fu M, Ren S, Wu X, Chen T, et al. Downregulation of miR-375 contributes to ERBB2-mediated VEGFA overexpression in esophageal cancer. J Cancer. 2021;12:7138-7146 pubmed publisher
Li S, Qin C, Chen Y, Wei D, Tan Z, Meng J. Implications of cell division cycle associated 4 on the Wilm's tumor cells viability via AKT/mTOR signaling pathway. Ren Fail. 2021;43:1470-1478 pubmed publisher
Li Q, Wang Y, He J. MiR-133a-3p attenuates resistance of non-small cell lung cancer cells to gefitinib by targeting SPAG5. J Clin Lab Anal. 2021;35:e23853 pubmed publisher
Wu Y, Huang Y, Zhang W, Gunst S. The proprotein convertase furin inhibits IL-13-induced inflammation in airway smooth muscle by regulating integrin-associated signaling complexes. Am J Physiol Lung Cell Mol Physiol. 2021;321:L102-L115 pubmed publisher
Montt Guevara M, Finiguerra M, Marzi I, Fidecicchi T, Ferrari A, Genazzani A, et al. D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes. Front Endocrinol (Lausanne). 2021;12:660815 pubmed publisher
Alzahrani A, Rajendran P, Veeraraghavan V, Hanieh H. Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways. Int J Mol Sci. 2021;22: pubmed publisher
Wang H, Chinnathambi A, Alahmadi T, Alharbi S, Veeraraghavan V, Krishna Mohan S, et al. Phyllanthin inhibits MOLT-4 leukemic cancer cell growth and induces apoptosis through the inhibition of AKT and JNK signaling pathway. J Biochem Mol Toxicol. 2021;35:1-10 pubmed publisher
Kopietz F, Alshuweishi Y, Bijland S, Alghamdi F, Degerman E, Sakamoto K, et al. A-769662 inhibits adipocyte glucose uptake in an AMPK-independent manner. Biochem J. 2021;478:633-646 pubmed publisher
Paul R, Whiteman K, Falconer S, Oldham J, Jeanplong F, Matthews K, et al. IGF1 does not overcome sexual dimorphism of body and muscle size in Mstn-/- mice. J Endocrinol. 2021;248:207-220 pubmed publisher
Pucci S, Fasoli F, Moretti M, Benfante R, Di Lascio S, Viani P, et al. Choline and nicotine increase glioblastoma cell proliferation by binding and activating α7- and α9- containing nicotinic receptors. Pharmacol Res. 2021;163:105336 pubmed publisher
Baltazar Lara R, Ávila Mendoza J, Martínez Moreno C, Carranza M, Pech Pool S, Vázquez Martínez O, et al. Neuroprotective Effects of Growth Hormone (GH) and Insulin-Like Growth Factor Type 1 (IGF-1) after Hypoxic-Ischemic Injury in Chicken Cerebellar Cell Cultures. Int J Mol Sci. 2020;22: pubmed publisher
Rajendran P, Ammar R, Al Saeedi F, Mohamed M, ElNaggar M, Al ramadan S, et al. Kaempferol Inhibits Zearalenone-Induced Oxidative Stress and Apoptosis via the PI3K/Akt-Mediated Nrf2 Signaling Pathway: In Vitro and In Vivo Studies. Int J Mol Sci. 2020;22: pubmed publisher
Liu B, Sun W, Gao W, Li L, Cao Z, Yang X, et al. microRNA-451a promoter methylation regulated by DNMT3B expedites bladder cancer development via the EPHA2/PI3K/AKT axis. BMC Cancer. 2020;20:1019 pubmed publisher
Kopietz F, Rupar K, Berggreen C, S xe4 ll J, Vertommen D, Degerman E, et al. Inhibition of AMPK activity in response to insulin in adipocytes: involvement of AMPK pS485, PDEs, and cellular energy levels. Am J Physiol Endocrinol Metab. 2020;319:E459-E471 pubmed publisher
Huang Y, Gunst S. Phenotype transitions induced by mechanical stimuli in airway smooth muscle are regulated by differential interactions of parvin isoforms with paxillin and Akt. Am J Physiol Lung Cell Mol Physiol. 2020;318:L1036-L1055 pubmed publisher
Parvathaneni V, Kulkarni N, Shukla S, Farrales P, Kunda N, Muth A, et al. Systematic Development and Optimization of Inhalable Pirfenidone Liposomes for Non-Small Cell Lung Cancer Treatment. Pharmaceutics. 2020;12: pubmed publisher
Gutierrez Repiso C, Ho Plagaro A, Santiago Fernández C, Garcia Serrano S, Rodriguez Pacheco F, Valdes S, et al. Jejunal Insulin Signalling Is Increased in Morbidly Obese Subjects with High Insulin Resistance and Is Regulated by Insulin and Leptin. J Clin Med. 2020;9: pubmed publisher
Inprasit C, Huang Y, Lin Y. Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model. Int J Mol Med. 2020;45:779-792 pubmed publisher
Zhang C, Smith M, Zhou G, Lai A, Hoy R, Mroz V, et al. Phlpp1 is associated with human intervertebral disc degeneration and its deficiency promotes healing after needle puncture injury in mice. Cell Death Dis. 2019;10:754 pubmed publisher
Chalenko Y, Kalinin E, Marchenkov V, Sysolyatina E, Surin A, Sobyanin K, et al. Phylogenetically Defined Isoforms of Listeria monocytogenes Invasion Factor InlB Differently Activate Intracellular Signaling Pathways and Interact with the Receptor gC1q-R. Int J Mol Sci. 2019;20: pubmed publisher
Yu C, Chu S, Yang S, Hsieh Y, Lee C, Chen P. Induction of apoptotic but not autophagic cell death by Cinnamomum cassia extracts on human oral cancer cells. J Cell Physiol. 2019;234:5289-5303 pubmed publisher
Sferra R, Pompili S, Ventura L, Dubuquoy C, Speca S, Gaudio E, et al. Interaction between sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-β/Smads pathways in experimental intestinal fibrosis. An in vivo immunohistochemical study. Eur J Histochem. 2018;62: pubmed publisher
Hansen J, de Marè S, Jones H, Göransson O, Lindkvist Petersson K. Visualization of lipid directed dynamics of perilipin 1 in human primary adipocytes. Sci Rep. 2017;7:15011 pubmed publisher
Eritano A, Altamirano A, Beyeler S, Gaytan N, Velasquez M, Riggs B. The endoplasmic reticulum is partitioned asymmetrically during mitosis before cell fate selection in proneuronal cells in the early Drosophila embryo. Mol Biol Cell. 2017;28:1530-1538 pubmed publisher
Sackstein R, Schatton T, Barthel S. T-lymphocyte homing: an underappreciated yet critical hurdle for successful cancer immunotherapy. Lab Invest. 2017;97:669-697 pubmed publisher
Chen W, Tang X, Yang X, Weng C, Yang K, Wen J, et al. Effects and Mechanisms of Radiofrequency Ablation of Renal Sympathetic Nerve on Anti-Hypertension in Canine. Arq Bras Cardiol. 2017;108:237-245 pubmed publisher
Kumari P, Srivastava A, Ghosh E, Ranjan R, Dogra S, Yadav P, et al. Core engagement with ?-arrestin is dispensable for agonist-induced vasopressin receptor endocytosis and ERK activation. Mol Biol Cell. 2017;28:1003-1010 pubmed publisher
Hatakeyama H, Nakahata Y, Yarimizu H, Kanzaki M. Live-cell single-molecule labeling and analysis of myosin motors with quantum dots. Mol Biol Cell. 2017;28:173-181 pubmed publisher
Zandvakili I, Lin Y, Morris J, Zheng Y. Rho GTPases: Anti- or pro-neoplastic targets?. Oncogene. 2017;36:3213-3222 pubmed publisher
Kaiser T, Poehn B, Szkiba D, Preussner M, Sedlazeck F, Zrim A, et al. The genomic basis of circadian and circalunar timing adaptations in a midge. Nature. 2016;540:69-73 pubmed publisher
Ai J, Pascal L, Wei L, Zang Y, Zhou Y, Yu X, et al. EAF2 regulates DNA repair through Ku70/Ku80 in the prostate. Oncogene. 2017;36:2054-2065 pubmed publisher
Mulay A, Akram K, Williams D, Armes H, Russell C, Hood D, et al. An in vitro model of murine middle ear epithelium. Dis Model Mech. 2016;9:1405-1417 pubmed
Maksimov V, Wäneskog M, Rodriguez A, Bjerling P. Stress sensitivity of a fission yeast strain lacking histidine kinases is rescued by the ectopic expression of Chk1 from Candida albicans. Curr Genet. 2017;63:343-357 pubmed publisher
XU C, Fu X, Zhu S, Liu J. Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF-induced TrkB endocytosis and dendrite outgrowth. Mol Biol Cell. 2016;27:3342-3356 pubmed
Mowers E, Sharifi M, Macleod K. Autophagy in cancer metastasis. Oncogene. 2017;36:1619-1630 pubmed publisher
Gritsman K, Yuzugullu H, Von T, Yan H, Clayton L, Fritsch C, et al. Hematopoiesis and RAS-driven myeloid leukemia differentially require PI3K isoform p110?. J Clin Invest. 2014;124:1794-809 pubmed publisher
Lagunas L, Clipstone N. Deregulated NFATc1 activity transforms murine fibroblasts via an autocrine growth factor-mediated Stat3-dependent pathway. J Cell Biochem. 2009;108:237-48 pubmed publisher
Gude N, Emmanuel G, Wu W, Cottage C, Fischer K, Quijada P, et al. Activation of Notch-mediated protective signaling in the myocardium. Circ Res. 2008;102:1025-35 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-AKT1 (Ser473) Monoclonal Antibody (14-6)
Catalog # :
44-621G
Quantity :
100 µL
Price :
US 514
Clonality :
Monoclonal
Purity :
protein A
Host :
Rabbit
Reactivity :
Human, Mouse
Applications :
Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000
Species :
Human, Mouse
Clone :
14-6
Isotype :
IgG
Storage :
-20°C
Description :
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
Immunogen :
The antibody was produced against a chemically synthesized phosphopeptide derived from the region of human Akt that contains serine 473.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000
Aliases :
Akt; Akt kinase; AKT serine/threonine kinase 1; Akt/PKB; akt1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; CAKT; C-AKT; CG4006; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; dAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAKT1; DAKT1/PKB; Dmel CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; P-AKT; PKB; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; protein kinase B; Protein kinase B alpha; protein kinase B-alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine protein kinase; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; SD10374p; serine/threonine protein kinase; serine-threonine protein kinase; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA