Phospho-IRS1 (Ser616) Polyclonal Antibody | Invitrogen 44-550G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-IRS1 (Ser616) Polyclonal Antibody

catalog :

44-550G

quantity :

100 µL

price :

US 450.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 1f	Erlandsson M, Erdogan S, Was xe9 n C, Andersson K, Silfversw xe4 rd S, Pullerits R, et al. IGF1R signalling is a guardian of self-tolerance restricting autoantibody production. Front Immunol. 2022;13:958206 pubmed publisher
	Mietelska Porowska A, Doma x144 ska J, Want A, Wi x119 ckowska Gacek A, Chutora x144 ski D, Koperski M, et al. Induction of Brain Insulin Resistance and Alzheimer's Molecular Changes by Western Diet. Int J Mol Sci. 2022;23: pubmed publisher
	Arvanitakis Z, Capuano A, Wang H, Schneider J, Kapasi A, Bennett D, et al. Brain insulin signaling and cerebrovascular disease in human postmortem brain. Acta Neuropathol Commun. 2021;9:71 pubmed publisher
	Chou S, Chan L, Chung C, Chiu J, Hsieh Y, Hong C. Altered Insulin Receptor Substrate 1 Phosphorylation in Blood Neuron-Derived Extracellular Vesicles From Patients With Parkinson's Disease. Front Cell Dev Biol. 2020;8:564641 pubmed publisher
	Denver P, English A, McClean P. Inflammation, insulin signaling and cognitive function in aged APP/PS1 mice. Brain Behav Immun. 2018;70:423-434 pubmed publisher
	Velázquez R, Tran A, Ishimwe E, Denner L, Dave N, Oddo S, et al. Central insulin dysregulation and energy dyshomeostasis in two mouse models of Alzheimer's disease. Neurobiol Aging. 2017;58:1-13 pubmed publisher
	Garwood C, Ratcliffe L, Morgan S, Simpson J, Owens H, Vazquez VillaseÃ±or I, et al. Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors. Mol Brain. 2015;8:51 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-IRS1 (Ser616) Polyclonal Antibody

Catalog # :

44-550G

Quantity :

100 µL

Price :

US 450.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20°C

Description :

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

Immunogen :

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of IRS-1 that contains serine 616. The sequence is conserved in many species including human, mouse, and rat

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:1,000

Aliases :

G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185

more info or order :

Invitrogen product webpage