Phospho-MYLK (Ser19) Polyclonal Antibody | Invitrogen 44-260G product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-MYLK (Ser19) Polyclonal Antibody

catalog :

44-260G

quantity :

100 µL

price :

US 466

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, chicken

application :

western blot

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4	Marcos Ramiro B, GarcÃa Weber D, Barroso S, Feito J, Ortega M, Cernuda MorollÃ³n E, et al. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border. J Cell Biol. 2016;213:385-402 pubmed publisher
	Zhang Y, Liu B, Luo X, Zhang J, Li N, Ma Q, et al. A novel function of nuclear nonmuscle myosin regulatory light chain in promotion of xanthine oxidase transcription after myocardial ischemia/reperfusion. Free Radic Biol Med. 2015;83:115-28 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-MYLK (Ser19) Polyclonal Antibody

Catalog # :

44-260G

Quantity :

100 µL

Price :

US 466

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Chicken, Human

Applications :

Western Blot: 1:1,000

Species :

Chicken, Human

Isotype :

IgG

Storage :

-20°C

Description :

MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Immunogen :

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of MLC that contains serine 19 (serine 20 including the initiating methionine).

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1:1,000

Aliases :

155 kda myosin light chain kinase homolog; 9530072E15Rik; A clone isolated from a chicken gizzard cDNA library which has high homology with the 3'-end sequence of the chicken gizzard myosin light chain kinase (MLCK) cDNA; The 5'-leader seqeuence derives from the intronic sequence of the MLCK gene.; A930019C19Rik; AAT7; AW489456; kinase related protein; kinase-related protein; KRP; KRP/Telokin; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; mylk; MYLK1; myosin light chain kinase; myosin light chain kinase, smooth muscle; Myosin light chain kinase, smooth muscle, deglutamylated form; myosin, light polypeptide kinase; nmMlck; smMLCK; smooth muscle myosin light chain kinase; smooth muscle myosin light chain kinase (61-kDa active fragment); smooth muscle myosin light chain kinase precursor (EC 2.7.2.37); telokin; unnamed protein product

more info or order :

Invitrogen product webpage