product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody
catalog :
44-244G
quantity :
100 µL
price :
US 500.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 29
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 1b
Luckett K, Cracchiolo J, Krishnamoorthy G, Leandro García L, Nagarajah J, Saqcena M, et al. Co-inhibition of SMAD and MAPK signaling enhances 124I uptake in BRAF-mutant thyroid cancers. Endocr Relat Cancer. 2021;28:391-402 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:150; loading ...; fig 6g
Quilichini E, Fabre M, Dirami T, Stedman A, De Vas M, Ozguc O, et al. Pancreatic ductal deletion of Hnf1b disrupts exocrine homeostasis, leads to pancreatitis and facilitates tumorigenesis. Cell Mol Gastroenterol Hepatol. 2019;: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 4a
Ruiz Gutierrez M, Bölükbaşı Ö, Alexe G, Kotini A, Ballotti K, Joyce C, et al. Therapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells. JCI Insight. 2019;5: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s5d
Boutin A, Liao W, Wang M, Hwang S, Karpinets T, Cheung H, et al. Oncogenic Kras drives invasion and maintains metastases in colorectal cancer. Genes Dev. 2017;31:370-382 pubmed publisher
  • western blot; mouse; loading ...; fig 4a
Kocsis T, Trencsenyi G, Szabó K, Baán J, Müller G, Mendler L, et al. Myostatin propeptide mutation of the hypermuscular Compact mice decreases the formation of myostatin and improves insulin sensitivity. Am J Physiol Endocrinol Metab. 2017;312:E150-E160 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 2d
Liang Y, Zhu F, Zhang H, Chen D, Zhang X, Gao Q, et al. Conditional ablation of TGF-? signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model. Sci Rep. 2016;6:29479 pubmed publisher
  • western blot; human; fig 3
Margaryan N, Gilgur A, Seftor E, Purnell C, Arva N, Gosain A, et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci. 2016;17:418 pubmed publisher
Li Z, Jiao X, Robertson A, Di Sante G, Ashton A, DiRocco A, et al. The DACH1 gene is frequently deleted in prostate cancer, restrains prostatic intraepithelial neoplasia, decreases DNA damage repair, and predicts therapy responses. Oncogene. 2023;42:1857-1873 pubmed publisher
Ge Q, Shi Z, Zou K, Ying J, Chen J, Yuan W, et al. Protein phosphatase PPM1A inhibition attenuates osteoarthritis via regulating TGF-β/Smad2 signaling in chondrocytes. JCI Insight. 2023;8: pubmed publisher
Fang C, Chen S, Huang C, Liao Y, Chao S, Yu C. Fucoidan-Mediated Inhibition of Fibrotic Properties in Oral Submucous Fibrosis via the MEG3/miR-181a/Egr1 Axis. Pharmaceuticals (Basel). 2022;15: pubmed publisher
Song I, Nagamani S, Nguyen D, Grafe I, Sutton V, Gannon F, et al. Targeting TGF-β for treatment of osteogenesis imperfecta. J Clin Invest. 2022;132: pubmed publisher
Deng R, Li C, Wang X, Chang L, Ni S, Zhang W, et al. Periosteal CD68+ F4/80+ Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-β1. Adv Sci (Weinh). 2022;9:e2103343 pubmed publisher
Schilpp C, Lochbaum R, Braubach P, Jonigk D, Frick M, Dietl P, et al. TGF-β1 increases permeability of ciliated airway epithelia via redistribution of claudin 3 from tight junction into cell nuclei. Pflugers Arch. 2021;473:287-311 pubmed publisher
Yoon J, Lee H, Kim H, Han B, Lee H, Lee Y, et al. Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation. Int J Mol Sci. 2020;21: pubmed publisher
Gabitova Cornell L, Surumbayeva A, Peri S, Franco Barraza J, Restifo D, Weitz N, et al. Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer. Cancer Cell. 2020;38:567-583.e11 pubmed publisher
Ghiroldi A, Piccoli M, Creo P, Cirillo F, Rota P, D Imperio S, et al. Role of sialidase Neu3 and ganglioside GM3 in cardiac fibroblasts activation. Biochem J. 2020;477:3401-3415 pubmed publisher
Brkić J, Dunk C, Shan Y, O Brien J, Lye P, Qayyum S, et al. Differential Role of Smad2 and Smad3 in the Acquisition of an Endovascular Trophoblast-Like Phenotype and Preeclampsia. Front Endocrinol (Lausanne). 2020;11:436 pubmed publisher
Watanabe H, Goto S, Kato R, Komiyama S, Nagaoka Y, Kazama T, et al. The neovascularization effect of dedifferentiated fat cells. Sci Rep. 2020;10:9211 pubmed publisher
Wójcik Pszczoła K, Chłoń Rzepa G, Jankowska A, Ślusarczyk M, Ferdek P, Kusiak A, et al. A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling. Int J Mol Sci. 2020;21: pubmed publisher
Chamberlin T, Thompson V, Hillers Ziemer L, Walton B, Arendt L. Obesity reduces mammary epithelial cell TGFβ1 activity through macrophage-mediated extracellular matrix remodeling. FASEB J. 2020;34:8611-8624 pubmed publisher
Sami E, Paul B, Koziol J, ElShamy W. The Immunosuppressive Microenvironment in BRCA1-IRIS-Overexpressing TNBC Tumors Is Induced by Bidirectional Interaction with Tumor-Associated Macrophages. Cancer Res. 2020;80:1102-1117 pubmed publisher
Omene C, Ma L, Moore J, Ouyang H, Illa Bochaca I, Chou W, et al. Aggressive Mammary Cancers Lacking Lymphocytic Infiltration Arise in Irradiated Mice and Can Be Prevented by Dietary Intervention. Cancer Immunol Res. 2020;8:217-229 pubmed publisher
Wegner K, Mueller B, Unterberger C, Avila E, Ruetten H, Turco A, et al. Prostate epithelial-specific expression of activated PI3K drives stromal collagen production and accumulation. J Pathol. 2020;250:231-242 pubmed publisher
Arif E, Solanki A, Srivastava P, Rahman B, Tash B, Holzman L, et al. The motor protein Myo1c regulates transforming growth factor-β-signaling and fibrosis in podocytes. Kidney Int. 2019;96:139-158 pubmed publisher
Zhang Q, Wang C, Cliby W. Cancer-associated stroma significantly contributes to the mesenchymal subtype signature of serous ovarian cancer. Gynecol Oncol. 2019;152:368-374 pubmed publisher
Carnevale D, Facchinello N, Iodice D, Bizzotto D, Perrotta M, De Stefani D, et al. Loss of EMILIN-1 Enhances Arteriolar Myogenic Tone Through TGF-β (Transforming Growth Factor-β)-Dependent Transactivation of EGFR (Epidermal Growth Factor Receptor) and Is Relevant for Hypertension in Mice and Humans. Arterioscler Thromb Vasc Biol. 2018;38:2484-2497 pubmed publisher
Caja L, Tzavlaki K, Dadras M, Tan E, Hatem G, Maturi N, et al. Snail regulates BMP and TGFβ pathways to control the differentiation status of glioma-initiating cells. Oncogene. 2018;37:2515-2531 pubmed publisher
David J, Dominguez C, McCampbell K, Gulley J, Schlom J, Palena C. A novel bifunctional anti-PD-L1/TGF-? Trap fusion protein (M7824) efficiently reverts mesenchymalization of human lung cancer cells. Oncoimmunology. 2017;6:e1349589 pubmed publisher
Strizzi L, Sandomenico A, Margaryan N, Focà A, Sanguigno L, Bodenstine T, et al. Effects of a novel Nodal-targeting monoclonal antibody in melanoma. Oncotarget. 2015;6:34071-86 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody
Catalog # :
44-244G
Quantity :
100 µL
Price :
US 500.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Human, Mouse
Applications :
ChIP Assay: 10 µL, Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1:1,000
Species :
Human, Mouse
Isotype :
IgG
Storage :
-20°C
Description :
SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.
Immunogen :
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Smad2 that contains serines 465 and 467. The sequence is conserved in mouse and rat.
Format :
Liquid
Applications w/Dilutions :
ChIP Assay: 10 µL, Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1:1,000
Aliases :
7120426M23Rik; EGK_09664; hMAD-2; hSMAD2; JV18; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Madh2; Madr2; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against decapentaplegic-like protein 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2; Smad-2; Smad2Deltaexon3
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA