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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody
catalog :
44-1150G
quantity :
100 µL
price :
US 500.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 9
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 6g
Gyamfi J, Yeo J, Kwon D, Min B, Cha Y, Koo J, et al. Interaction between CD36 and FABP4 modulates adipocyte-induced fatty acid import and metabolism in breast cancer. NPJ Breast Cancer. 2021;7:129 pubmed publisher
  • flow cytometry; human; loading ...; fig 4e, 4f
Wen Z, Jin K, Shen Y, Yang Z, Li Y, Wu B, et al. N-myristoyltransferase deficiency impairs activation of kinase AMPK and promotes synovial tissue inflammation. Nat Immunol. 2019;20:313-325 pubmed publisher
Kim J, Lee H, Jeong J, Yoon Y, Lee I, Kim J, et al. OR2AT4, an Ectopic Olfactory Receptor, Suppresses Oxidative Stress-Induced Senescence in Human Keratinocytes. Antioxidants (Basel). 2022;11: pubmed publisher
Richter R, Ashtekar A, Zheng L, Pretorius D, Kaushlendra T, Sanderson R, et al. Glycocalyx heparan sulfate cleavage promotes endothelial cell angiopoietin-2 expression by impairing shear stress-related AMPK/FoxO1 signaling. JCI Insight. 2022;7: pubmed publisher
Cheng L, Zhu X, Liu Y, Zhu K, Lin K, Li F. ACSL4 contributes to sevoflurane-induced ferroptotic neuronal death in SH-SY5Y cells via the 5' AMP-activated protein kinase/mammalian target of rapamycin pathway. Ann Transl Med. 2021;9:1454 pubmed publisher
Wu T, Chen Y, Ho C, Yeh T, Sun G, Tseng C, et al. 3H-1,2-Dithiole-3-Thione Protects Lens Epithelial Cells against Fructose-Induced Epithelial-Mesenchymal Transition via Activation of AMPK to Eliminate AKR1B1-Induced Oxidative Stress in Diabetes Mellitus. Antioxidants (Basel). 2021;10: pubmed publisher
Nguyen K, Rivera A, Alzoubi M, Wathieu H, Dong S, Yousefi H, et al. Evaluation of liver kinase B1 downstream signaling expression in various breast cancers and relapse free survival after systemic chemotherapy treatment. Oncotarget. 2021;12:1110-1115 pubmed publisher
Wu T, Chen Y, Chang H, Kung Y, Tseng C, Cheng P. AKR1B1-Induced Epithelial-Mesenchymal Transition Mediated by RAGE-Oxidative Stress in Diabetic Cataract Lens. Antioxidants (Basel). 2020;9: pubmed publisher
Yin H, Zhao L, Li S, Xu L, Wang Y, Chen H. Impaired Cellular Energy Metabolism Contributes to Duck-Enteritis-Virus-Induced Autophagy via the AMPK-TSC2-MTOR Signaling Pathway. Front Cell Infect Microbiol. 2017;7:423 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody
Catalog # :
44-1150G
Quantity :
100 µL
Price :
US 500.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Human, Mouse
Applications :
Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:10-1:50, Western Blot: 1:1,000
Species :
Human, Mouse
Isotype :
IgG
Storage :
-20°C
Description :
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Immunogen :
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human AMPK alpha 1 that contains threonine 183 and AMPK alpha 2 that contains threonine 172. The sequence is conserved in human, mouse and rat.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:10-1:50, Western Blot: 1:1,000
Aliases :
2310008I11Rik; 5'-AMP-activated protein kinase alpha-1 catalytic subunit; 5'-AMP-activated protein kinase alpha-2 catalytic subunit; 5'-AMP-activated protein kinase catalytic alpha-2 subunit; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; 63 kDa subunit; A830082D05; AAPK2; ACACA kinase; acetyl-CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha 2; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK 63 kDa subunit; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPK-activated protein kinase alpha-2 subunit; AMPKalpha1; AMPK-alpha-1 chain; AMPKalpha2; AMPK-alpha-2 chain; C130083N04Rik; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; pAMPK; PRKAA; Prkaa1; Prkaa2; protein kinase; protein kinase AMP-activated alpha 2 catalytic subunit; protein kinase AMP-activated catalytic subunit alpha 1; protein kinase AMP-activated catalytic subunit alpha 2; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; tau-protein kinase PRKAA1
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA