product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GAPDH Monoclonal Antibody (258)
catalog :
437000
quantity :
100 µg
price :
US 401
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
258
reactivity :
human, mouse, bovine, pigs , cat, domestic sheep, domestic horse, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 18
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:10,000; loading ...; fig 6a
Bodrug T, Wilson Kubalek E, Nithianantham S, Thompson A, Alfieri A, Gaska I, et al. The kinesin-5 tail domain directly modulates the mechanochemical cycle of the motor domain for anti-parallel microtubule sliding. elife. 2020;9: pubmed publisher
  • western blot; human; fig s2d
Soragni A, Janzen D, Johnson L, Lindgren A, Thai Quynh Nguyen A, Tiourin E, et al. A Designed Inhibitor of p53 Aggregation Rescues p53 Tumor Suppression in Ovarian Carcinomas. Cancer Cell. 2016;29:90-103 pubmed publisher
  • western blot; human; 1:10,000; fig 7
Chaki S, Barhoumi R, Rivera G. Actin remodeling by Nck regulates endothelial lumen formation. Mol Biol Cell. 2015;26:3047-60 pubmed publisher
  • western blot; mouse; 1:10000
Stauch K, Purnell P, Fox H. Quantitative proteomics of synaptic and nonsynaptic mitochondria: insights for synaptic mitochondrial vulnerability. J Proteome Res. 2014;13:2620-36 pubmed publisher
  • western blot; human; 1:10000; fig 3
Villeneuve L, Tiede L, Morsey B, Fox H. Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone. J Proteome Res. 2013;12:4599-606 pubmed publisher
  • western blot; human; 1:10000; fig 4
Chaki S, Barhoumi R, Berginski M, Sreenivasappa H, Trache A, Gomez S, et al. Nck enables directional cell migration through the coordination of polarized membrane protrusion with adhesion dynamics. J Cell Sci. 2013;126:1637-49 pubmed publisher
  • western blot; rhesus macaque
Delpeut S, Rudd P, Labonte P, von Messling V. Membrane fusion-mediated autophagy induction enhances morbillivirus cell-to-cell spread. J Virol. 2012;86:8527-35 pubmed publisher
  • western blot; human; fig 5
Hancock M, Hermanson S, Dolman N. A quantitative TR-FRET plate reader immunoassay for measuring autophagy. Autophagy. 2012;8:1227-44 pubmed publisher
Magdaleno C, Dixon L, Rajasekaran N, Varadaraj A. HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl2. Sci Rep. 2020;10:18560 pubmed publisher
Sharma A, Dagar S, Mylavarapu S. Transgelin-2 and phosphoregulation of the LIC2 subunit of dynein govern mitotic spindle orientation. J Cell Sci. 2020;133: pubmed publisher
Dhanjal N, Sharma S, Prabhu K, Prakash N. Selenium supplementation through Se-rich dietary matrices can upregulate the anti-inflammatory responses in lipopolysaccharide-stimulated murine macrophages. Food Agric Immunol. 2017;28:1374-1392 pubmed publisher
Small J, Washburn E, Millington K, Zhu J, Holder S. The addition of abemaciclib to sunitinib induces regression of renal cell carcinoma xenograft tumors. Oncotarget. 2017;8:95116-95134 pubmed publisher
Villasante A, Sakaguchi K, Kim J, Cheung N, Nakayama M, Parsa H, et al. Vascularized Tissue-Engineered Model for Studying Drug Resistance in Neuroblastoma. Theranostics. 2017;7:4099-4117 pubmed publisher
Marturano Kruik A, Villasante A, Yaeger K, Ambati S, Chramiec A, Raimondi M, et al. Biomechanical regulation of drug sensitivity in an engineered model of human tumor. Biomaterials. 2018;150:150-161 pubmed publisher
Villasante A, Marturano Kruik A, Ambati S, Liu Z, Godier Furnemont A, Parsa H, et al. Recapitulating the Size and Cargo of Tumor Exosomes in a Tissue-Engineered Model. Theranostics. 2016;6:1119-30 pubmed publisher
Ratnikov B, Scott D, Osterman A, Smith J, Ronai Z. Metabolic rewiring in melanoma. Oncogene. 2017;36:147-157 pubmed publisher
Purnell P, Fox H. Autophagy-mediated turnover of dynamin-related protein 1. BMC Neurosci. 2013;14:86 pubmed publisher
Li Y, Lin L, Li Z, Ye X, Xiong K, Aryal B, et al. Iron homeostasis regulates the activity of the microRNA pathway through poly(C)-binding protein 2. Cell Metab. 2012;15:895-904 pubmed publisher
product information
Product Type :
Antibody
Product Name :
GAPDH Monoclonal Antibody (258)
Catalog # :
437000
Quantity :
100 µg
Price :
US 401
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Bovine, Equine, Feline, Human, Non-human primate, Ovine, Porcine
Applications :
Immunocytochemistry: 1-2 µg/mL, Immunohistochemistry (Paraffin): 1:50-1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1-2 µg/mL
Species :
Bovine, Equine, Feline, Human, Non-human primate, Ovine, Porcine
Clone :
258
Isotype :
IgG1
Storage :
-20°C
Description :
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
Immunogen :
Recombinant protein derived from the C-terminus region of human GAPDH protein
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1-2 µg/mL, Immunohistochemistry (Paraffin): 1:50-1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1-2 µg/mL
Aliases :
38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; bb02e05; cb350; cb609; CDABP0047; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; fb71f08; fk58c09; G3PD; G3PDH; GAPD; GAPD2; gapdh; GAPDH2; GAPDH-2; GAPDHS; Gapds; Gapd-s; glceraldehyde-3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase (G3PDH); glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase GAPDH; glyceraldehyde-3-phosphate dehydrogenase like-17 protein; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; glyceraldehyde-3-phosphate dehydrogenase, testis-specific; glyceraldehyde-phosphate-dehydrogenase; glycerine aldehyde 3-phosphate dehydrogenase; HEL-S-162eP; HEL-S-278; HGNC:4141; HSD35; HSD-35; I79_001391; KNC-NDS6; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; mg:bb02e05; MGC128279 protein; MGC88685; multifunctional protein, glycolytic enzyme; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; similar to glyceraldehyde 3-phosphate dehydrogenase; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; Unknown (protein for IMAGE:8101613); unnamed protein product; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA