FXR Monoclonal Antibody (A9033A) | Invitrogen 417200 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

FXR Monoclonal Antibody (A9033A)

catalog :

417200

quantity :

100 µL

price :

US 556.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A9033A

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; loading ...; fig s1	Ijssennagger N, van Rooijen K, Magnúsdóttir S, Ramos Pittol J, Willemsen E, de Zoete M, et al. Ablation of liver Fxr results in an increased colonic mucus barrier in mice. JHEP Rep. 2021;3:100344 pubmed publisher
immunocytochemistry; rat western blot; rat	Sawitza I, Kordes C, GÃ¶tze S, Herebian D, HÃ¤ussinger D. Bile acids induce hepatic differentiation of mesenchymal stem cells. Sci Rep. 2015;5:13320 pubmed publisher
	Wang S, Sheng F, Zou L, Xiao J, Li P. Hyperoside attenuates non-alcoholic fatty liver disease in rats via cholesterol metabolism and bile acid metabolism. J Adv Res. 2021;34:109-122 pubmed publisher
	Q Bartlett A, Vesco K, Purnell J, Francisco M, Goddard E, Guan X, et al. Pregnancy and weaning regulate human maternal liver size and function. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
	Czarnecka A, Milewski K, Albrecht J, Zielinska M. The Status of Bile Acids and Farnesoid X Receptor in Brain and Liver of Rats with Thioacetamide-Induced Acute Liver Failure. Int J Mol Sci. 2020;21: pubmed publisher
	Ramos Pittol J, Milona A, Morris I, Willemsen E, van der Veen S, Kalkhoven E, et al. FXR Isoforms Control Different Metabolic Functions in Liver Cells via Binding to Specific DNA Motifs. Gastroenterology. 2020;159:1853-1865.e10 pubmed publisher
	Li H, Xi Y, Xin X, Tian H, Hu Y. Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis. Nutr Metab (Lond). 2020;17:34 pubmed publisher

product information

Product Type :

Antibody

Product Name :

FXR Monoclonal Antibody (A9033A)

Catalog # :

417200

Quantity :

100 µL

Price :

US 556.00

Clonality :

Monoclonal

Purity :

Ammonium sulfate precipitation

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 0.2 µg/mL, Immunohistochemistry (Frozen): 20-40 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL

Species :

Human, Mouse, Rat

Clone :

A9033A

Isotype :

IgG2a

Storage :

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Description :

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

Immunogen :

Baculovirus-expressed recombinant human FXR (2-126 aa).

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.2 µg/mL, Immunohistochemistry (Frozen): 20-40 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL

Aliases :

AI957360; BAR; bile acid receptor; farnesoid X activated receptor; farnesoid X nuclear receptor; farnesoid X-activated receptor; Farnesol receptor HRR-1; FXR; HRR1; HRR-1; hypothetical protein LOC540528; MGC163445; NR1H4; Nuclear receptor subfamily 1 group H member 4; nuclear receptor subfamily 1, group H, member 4; retinoid X receptor-interacting protein 14; RIP14; RXR-interacting protein 14; Rxrip14

more info or order :

Invitrogen product webpage