TRA-1-81 Monoclonal Antibody (cl.26) | Invitrogen 41-1100 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TRA-1-81 Monoclonal Antibody (cl.26)

catalog :

41-1100

quantity :

100 µg

price :

US 430

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

cl.26

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:300; loading ...; fig 4a	Battaglia R, Beltran A, Delic S, Dumitru R, Robinson J, Kabiraj P, et al. Site-specific phosphorylation and caspase cleavage of GFAP are new markers of Alexander disease severity. elife. 2019;8: pubmed publisher
immunocytochemistry; human; fig 1	Reboun M, Rybová J, Dobrovolny R, Vcelak J, Veselková T, Storkanova G, et al. X-Chromosome Inactivation Analysis in Different Cell Types and Induced Pluripotent Stem Cells Elucidates the Disease Mechanism in a Rare Case of Mucopolysaccharidosis Type II in a Female. Folia Biol (Praha). 2016;62:82-9 pubmed
immunocytochemistry; human; 1:100; fig 1	Alves C, Dariolli R, Jorge F, Monteiro M, Maximino J, Martins R, et al. Gene expression profiling for human iPS-derived motor neurons from sporadic ALS patients reveals a strong association between mitochondrial functions and neurodegeneration. Front Cell Neurosci. 2015;9:289 pubmed publisher
western blot; human; 1:100	Wong A, Chin S, Xia S, Garner J, Bear C, Rossant J. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. Nat Protoc. 2015;10:363-81 pubmed publisher
immunocytochemistry; human	Afzal M, Strande J. Generation of induced pluripotent stem cells from muscular dystrophy patients: efficient integration-free reprogramming of urine derived cells. J Vis Exp. 2015;:52032 pubmed publisher
immunocytochemistry; human; fig 8	Ruff D, Macarthur C, Tran H, Bergseid J, Tian J, Shannon M, et al. Applications of quantitative polymerase chain reaction protein assays during reprogramming. Stem Cells Dev. 2012;21:530-8 pubmed publisher
flow cytometry; human	Hotta A, Cheung A, Farra N, Vijayaragavan K, Séguin C, Draper J, et al. Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Nat Methods. 2009;6:370-6 pubmed publisher
	Chumarina M, Russ K, Azevedo C, Heuer A, Pihl M, Collin A, et al. Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene. Acta Neuropathol Commun. 2019;7:208 pubmed publisher
	Vosough M, Ravaioli F, Zabulica M, Capri M, Garagnani P, Franceschi C, et al. Applying hydrodynamic pressure to efficiently generate induced pluripotent stem cells via reprogramming of centenarian skin fibroblasts. PLoS ONE. 2019;14:e0215490 pubmed publisher
	Marote A, Pomeshchik Y, Collin A, Goldwurm S, Lamas N, Pinto L, et al. Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene. Stem Cell Res. 2018;28:44-47 pubmed publisher
	Marote A, Pomeshchik Y, Goldwurm S, Collin A, Lamas N, Pinto L, et al. Generation of an induced pluripotent stem cell line (CSC-44) from a Parkinson's disease patient carrying a compound heterozygous mutation (c.823C>T and EX6 del) in the PARK2 gene. Stem Cell Res. 2018;27:90-94 pubmed publisher
	Petkov S, Glage S, Niemann H. Mouse iPSC generated with porcine reprogramming factors as a model for studying the effects of non-silenced heterologous transgenes on pluripotency. J Stem Cells Regen Med. 2017;13:20-28 pubmed
	Favaedi R, Shahhoseini M, Pakzad M, Mollamohammadi S, Baharvand H. Comparative epigenetic evaluation of human embryonic stem and induced pluripotent cells. Int J Dev Biol. 2016;60:103-10 pubmed publisher
	Quintanilla R. Cellular characterization of human pluripotent stem cells. Methods Mol Biol. 2013;997:179-90 pubmed publisher
	MacArthur C, Fontes A, Ravinder N, Kuninger D, Kaur J, Bailey M, et al. Generation of human-induced pluripotent stem cells by a nonintegrating RNA Sendai virus vector in feeder-free or xeno-free conditions. Stem Cells Int. 2012;2012:564612 pubmed publisher
	Andrews P, Casper J, Damjanov I, Duggan Keen M, Giwercman A, Hata J, et al. Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours. Int J Cancer. 1996;66:806-16 pubmed

product information

Product Type :

Antibody

Product Name :

TRA-1-81 Monoclonal Antibody (cl.26)

Catalog # :

41-1100

Quantity :

100 µg

Price :

US 430

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 2 µg/mL, Immunoprecipitation: 2-3 µg/mL, Western Blot: 1-5 µg/mL

Species :

Human

Clone :

cl.26

Isotype :

IgM

Storage :

-20°C

Description :

TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

Immunogen :

2102Ep cl.2A6 human embryonal carcinoma cells

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 2 µg/mL, Immunoprecipitation: 2-3 µg/mL, Western Blot: 1-5 µg/mL

Aliases :

GCTM-2 antigen; Gp200; PC; PCLP; PCLP1; PCLP-1; Podocalyxin; podocalyxin like; podocalyxin-like; podocalyxin-like protein 1; PODXL; TRA180; tra1-81

more info or order :

Invitrogen product webpage