CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor™ 570, eBioscience | Invitrogen 41-0452-80 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor™ 570, eBioscience

catalog :

41-0452-80

quantity :

25 µg

price :

US 169.00

clonality :

monoclonal

host :

rat

conjugate :

eFluor 570

clone name :

RA3-6B2

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 46

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human immunohistochemistry; human	Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
immunohistochemistry; mouse; loading ...; fig 4i	Takahashi T, Asano Y, Sugawara K, Yamashita T, Nakamura K, Saigusa R, et al. Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma. J Exp Med. 2017;214:1129-1151 pubmed publisher
immunohistochemistry; mouse; 1:200; fig 4	Kishimoto M, Matsuda T, Yanase S, Katsumi A, Suzuki N, Ikejiri M, et al. Rhof promotes murine marginal zone B cell development. Nagoya J Med Sci. 2014;76:293-305 pubmed
immunohistochemistry; mouse; 1:200; fig 7	Matsuda T, Yanase S, Takaoka A, Maruyama M. The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation. Immun Ageing. 2015;12:1 pubmed publisher
	Guo L, Li N, Yang Z, Li H, Zheng H, Yang J, et al. Role of CXCL5 in Regulating Chemotaxis of Innate and Adaptive Leukocytes in Infected Lungs Upon Pulmonary Influenza Infection. Front Immunol. 2021;12:785457 pubmed publisher
	Wilfahrt D, Philips R, Lama J, Kizerwetter M, Shapiro M, McCue S, et al. Histone deacetylase 3 represses cholesterol efflux during CD4+ T-cell activation. elife. 2021;10: pubmed publisher
	Zhang Y, Paynter J, Sou C, Fourfouris T, Wang Y, Abraham C, et al. Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants. Sci Adv. 2021;7:eabj3107 pubmed publisher
	Jeanne A, Sarazin T, Charlé M, Moali C, Fichel C, Boulagnon Rombi C, et al. Targeting Ovarian Carcinoma with TSP-1:CD47 Antagonist TAX2 Activates Anti-Tumor Immunity. Cancers (Basel). 2021;13: pubmed publisher
	Belliere J, Casemayou A, Colliou E, El Hachem H, Kounde C, Piedrafita A, et al. Ibrutinib does not prevent kidney fibrosis following acute and chronic injury. Sci Rep. 2021;11:11985 pubmed publisher
	Zaccagnini G, Greco S, Longo M, Maimone B, Voellenkle C, Fuschi P, et al. Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia. Cell Death Dis. 2021;12:435 pubmed publisher
	Dobosz E, Lorenz G, Ribeiro A, Würf V, Wadowska M, Kotlinowski J, et al. Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation. Dis Model Mech. 2021;14: pubmed publisher
	Gaurav R, Mikuls T, Thiele G, Nelson A, Niu M, Guda C, et al. High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis. PLoS ONE. 2021;16:e0240707 pubmed publisher
	Mendes A, Gigan J, Rodríguez Rodrígues C, Choteau S, Sanséau D, Barros D, et al. Proteostasis in dendritic cells is controlled by the PERK signaling axis independently of ATF4. Life Sci Alliance. 2021;4: pubmed publisher
	Richmond B, Mansouri S, Serezani A, Novitskiy S, Blackburn J, Du R, et al. Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD. Mucosal Immunol. 2021;14:431-442 pubmed publisher
	Aung K, Akiyama K, Kunitomo M, Mun A, Tosa I, Nguyen H, et al. Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model. Int J Mol Sci. 2020;21: pubmed publisher
	Sinha D, Nag P, Nanayakkara D, Duijf P, Burgess A, Raninga P, et al. Cep55 overexpression promotes genomic instability and tumorigenesis in mice. Commun Biol. 2020;3:593 pubmed publisher
	Heimesaat M, Mousavi S, Kl xf8 ve S, Genger C, Weschka D, Tamas A, et al. Pituitary Adenylate Cyclase-Activating Polypeptide Alleviates Intestinal, Extra-Intestinal and Systemic Inflammatory Responses during Acute Campylobacter jejuni-induced Enterocolitis in Mice. Pathogens. 2020;9: pubmed publisher
	Heimesaat M, Genger C, Klove S, Weschka D, Mousavi S, Bereswill S. The Host-Specific Intestinal Microbiota Composition Impacts Campylobacter coli Infection in a Clinical Mouse Model of Campylobacteriosis. Pathogens. 2020;9: pubmed publisher
	Merlo L, Bowers J, Stefanoni T, Getts R, Mandik Nayak L. B-Cell-Targeted 3DNA Nanotherapy Against Indoleamine 2,3-Dioxygenase 2 (IDO2) Ameliorates Autoimmune Arthritis in a Preclinical Model. Clin Pathol. 2020;13:2632010X20951812 pubmed publisher
	Kreimann K, Jang M, Rong S, Greite R, von Vietinghoff S, Schmitt R, et al. Ischemia Reperfusion Injury Triggers CXCL13 Release and B-Cell Recruitment After Allogenic Kidney Transplantation. Front Immunol. 2020;11:1204 pubmed publisher
	Kawabe T, Yi J, Kawajiri A, Hilligan K, Fang D, Ishii N, et al. Requirements for the differentiation of innate T-bethigh memory-phenotype CD4+ T lymphocytes under steady state. Nat Commun. 2020;11:3366 pubmed publisher
	Cao H, Duan R, Hu J. Overcoming Immunological Challenges to Helper-Dependent Adenoviral Vector-Mediated Long-Term CFTR Expression in Mouse Airways. Genes (Basel). 2020;11: pubmed publisher
	Kløve S, Genger C, Mousavi S, Weschka D, Bereswill S, Heimesaat M. Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota. Pathogens. 2020;9: pubmed publisher
	Natsume C, Aoki N, Aoyama T, Senda K, Matsui M, Ikegami A, et al. Fucoxanthin Ameliorates Atopic Dermatitis Symptoms by Regulating Keratinocytes and Regulatory Innate Lymphoid Cells. Int J Mol Sci. 2020;21: pubmed publisher
	Núñez M, Wisser K, Veatch S. Synergistic factors control kinase-phosphatase organization in B-cells engaged with supported bilayers. Mol Biol Cell. 2020;31:667-682 pubmed publisher
	Broderick L, Yost S, Li D, McGeough M, Booshehri L, Guaderrama M, et al. Mutations in topoisomerase IIβ result in a B cell immunodeficiency. Nat Commun. 2019;10:3644 pubmed publisher
	Castro Dopico T, Dennison T, Ferdinand J, Mathews R, FLEMING A, Clift D, et al. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis. Immunity. 2019;50:1099-1114.e10 pubmed publisher
	Escher U, Giladi E, Dunay I, Bereswill S, Gozes I, Heimesaat M. Anti-inflammatory Effects of the Octapeptide NAP in Human Microbiota-Associated Mice Suffering from Subacute Ileitis. Eur J Microbiol Immunol (Bp). 2018;8:34-40 pubmed publisher
	von Klitzing E, Ekmekciu I, KÃ¼hl A, Bereswill S, Heimesaat M. Multidrug-resistant Pseudomonas aeruginosa aggravates inflammatory responses in murine chronic colitis. Sci Rep. 2018;8:6685 pubmed publisher
	Chen Y, Chaudhary N, Yang N, Granato A, Turner J, Howard S, et al. Microbial symbionts regulate the primary Ig repertoire. J Exp Med. 2018;215:1397-1415 pubmed publisher
	Emgård J, Kammoun H, García Cassani B, Chesne J, Parigi S, Jacob J, et al. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. Immunity. 2018;48:120-132.e8 pubmed publisher
	Machelart A, Khadrawi A, Demars A, Willemart K, De Trez C, Letesson J, et al. Chronic Brucella Infection Induces Selective and Persistent Interferon Gamma-Dependent Alterations of Marginal Zone Macrophages in the Spleen. Infect Immun. 2017;85: pubmed publisher
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	Qu X, Li J, Baldwin H. Postnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice. Biochem Biophys Res Commun. 2016;470:613-619 pubmed publisher
	Allaire J, Roy S, Ouellet C, Lemieux Ã, Jones C, Paquet M, et al. Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation. Int J Cancer. 2016;138:2700-12 pubmed publisher
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	Dabydeen S, Kang K, DÃaz Cruz E, Alamri A, Axelrod M, Bouker K, et al. Comparison of tamoxifen and letrozole response in mammary preneoplasia of ER and aromatase overexpressing mice defines an immune-associated gene signature linked to tamoxifen resistance. Carcinogenesis. 2015;36:122-32 pubmed publisher
	Kasama Y, Mizukami T, Kusunoki H, Peveling Oberhag J, Nishito Y, Ozawa M, et al. B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-?B signalling. PLoS ONE. 2014;9:e91373 pubmed publisher
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product information

Product Type :

Antibody

Product Name :

CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor™ 570, eBioscience

Catalog # :

41-0452-80

Quantity :

25 µg

Price :

US 169.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Frozen): 1 µg/mL, Immunohistochemistry (Paraffin): 2 µg/mL

Species :

Human, Mouse

Clone :

RA3-6B2

Isotype :

IgG2a, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Frozen): 1 µg/mL, Immunohistochemistry (Paraffin): 2 µg/mL

Aliases :

B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen

more info or order :

Invitrogen product webpage