Metadherin Polyclonal Antibody | Invitrogen 40-6500 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Metadherin Polyclonal Antibody

catalog :

40-6500

quantity :

100 µg

price :

US 500

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 3g immunoprecipitation; human; loading ...; fig 2b western blot; human; 1:1000; loading ...; fig 1b	Liu L, Guan H, Li Y, Ying Z, Wu J, Zhu X, et al. Astrocyte Elevated Gene 1 Interacts with Acetyltransferase p300 and c-Jun To Promote Tumor Aggressiveness. Mol Cell Biol. 2017;37: pubmed publisher
western blot; human; 1:500; fig 1	Yao J, Qin L, Miao S, Wang X, Wu X. Overexpression of miR-506 suppresses proliferation and promotes apoptosis of osteosarcoma cells by targeting astrocyte elevated gene-1. Oncol Lett. 2016;12:1840-1848 pubmed
	Shen M, Smith H, Wei Y, Jiang Y, Zhao S, Wang N, et al. Pharmacological disruption of the MTDH-SND1 complex enhances tumor antigen presentation and synergizes with anti-PD-1 therapy in metastatic breast cancer. Nat Cancer. 2022;3:60-74 pubmed publisher
	Shen M, Wei Y, Kim H, Wan L, Jiang Y, Hang X, et al. Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis. Nat Cancer. 2022;3:43-59 pubmed publisher
	Jung H, Kim A, Kim S, Park J, Hwang E. AEG-1 Regulates TWIK-1 Expression as an RNA-Binding Protein in Astrocytes. Brain Sci. 2021;11: pubmed publisher
	Li P, He Y, Chen T, Choy K, Chow T, Wong I, et al. Disruption of SND1-MTDH Interaction by a High Affinity Peptide Results in SND1 Degradation and Cytotoxicity to Breast Cancer Cells In Vitro and In Vivo. Mol Cancer Ther. 2021;20:76-84 pubmed publisher
	Bi J, Areecheewakul S, Li Y, Yang S, Zhang Y, Ebeid K, et al. MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol. 2019;155:349-358 pubmed publisher
	Suzuki K, Takano S, Yoshitomi H, Nishino H, Kagawa S, Shimizu H, et al. Metadherin promotes metastasis by supporting putative cancer stem cell properties and epithelial plasticity in pancreatic cancer. Oncotarget. 2017;8:66098-66111 pubmed publisher
	Li Y, Gonzalez Bosquet J, Yang S, Thiel K, Zhang Y, Liu H, et al. Role of metadherin in estrogen-regulated gene expression. Int J Mol Med. 2017;40:303-310 pubmed publisher
	Meng X, Yang S, Zhang Y, Wang X, Goodfellow R, Jia Y, et al. Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs. J Biol Chem. 2015;290:11853-64 pubmed publisher
	Li P, Feng L, Chen N, Ge X, Lv X, Lu K, et al. Metadherin contributes to the pathogenesis of chronic lymphocytic leukemia partially through Wnt/Î²-catenin pathway. Med Oncol. 2015;32:479 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Metadherin Polyclonal Antibody

Catalog # :

40-6500

Quantity :

100 µg

Price :

US 500

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Canine, Human, Mouse, Non-human primate

Applications :

Immunocytochemistry: 1-2 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL

Species :

Canine, Human, Mouse, Non-human primate

Isotype :

IgG

Storage :

-20°C

Description :

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

Immunogen :

Synthetic peptide derived from an internal region of the human, chimpanzee, mouse, and bovine metadherin (Mtdh, metastasis adhesion protein, LYsine-RIch CEACAM1 co-isolated (LYRIC), LYRIC/3D3, astrocyte elevated gene-1 (AEG-1)) protein, which differs from rat by one amino acid

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1-2 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL

Aliases :

2610103J23Rik; 3D3; 3D3/Lyric; AEG1; AEG-1; astrocyte elevated gene 1; astrocyte elevated gene-1 protein; AV353288; D8Bwg1112e; LYRIC; LYRIC/ MTDH; LYRIC/3D3; lysine-rich CEACAM1 co-isolated protein; Metadherin; metastasis adhesion protein; MTDH; Protein LYRIC

more info or order :

Invitrogen product webpage