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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Occludin Polyclonal Antibody
catalog :
40-4700
quantity :
100 µg
price :
US 450
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 57
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:100; loading ...; fig 5a
Fayad R, Rojas M, Partisani M, Finetti P, Dib S, Abélanet S, et al. EFA6B regulates a stop signal for collective invasion in breast cancer. Nat Commun. 2021;12:2198 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:150; fig 6a
  • western blot; rat; 1:300; fig 6g
Cao X, Shen L, Wu S, Yan C, Zhou Y, Xiong G, et al. Urban fine particulate matter exposure causes male reproductive injury through destroying blood-testis barrier (BTB) integrity. Toxicol Lett. 2017;266:1-12 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 4
Hurtado Alvarado G, Dominguez Salazar E, Velazquez Moctezuma J, Gómez González B. A2A Adenosine Receptor Antagonism Reverts the Blood-Brain Barrier Dysfunction Induced by Sleep Restriction. PLoS ONE. 2016;11:e0167236 pubmed publisher
  • western blot; dogs
Janosevic D, Axis J, Bacallao R, Amsler K. Occludin Content Modulates Hydrogen Peroxide-Induced Increase in Renal Epithelial Paracellular Permeability. J Cell Biochem. 2016;117:769-79 pubmed publisher
Singh N, Gromnicova R, Brachner A, Kraev I, Romero I, Neuhaus W, et al. A hydrogel model of the human blood-brain barrier using differentiated stem cells. PLoS ONE. 2023;18:e0283954 pubmed publisher
Zhu X, Ma T, Li Y, Zhang M, Zhao L, Liang J, et al. Fingolimod protects against neurovascular unit injury in a rat model of focal cerebral ischemia/reperfusion injury. Neural Regen Res. 2023;18:869-874 pubmed publisher
Zhou R, Li J, Wang R, Chen Z, Zhou F. Moderate systemic therapeutic hypothermia is insufficient to protect blood-spinal cord barrier in spinal cord injury. Front Neurol. 2022;13:1041099 pubmed publisher
Yin L, Huang G, Khan I, Su L, Xia W, Law B, et al. Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice. Chin Med. 2022;17:116 pubmed publisher
Singh R, Birru B, Veit J, Arrigali E, Serban M. Development and Characterization of an In Vitro Round Window Membrane Model for Drug Permeability Evaluations. Pharmaceuticals (Basel). 2022;15: pubmed publisher
Sifat A, Archie S, Nozohouri S, Villalba H, Zhang Y, Sharma S, et al. Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome. Fluids Barriers CNS. 2022;19:74 pubmed publisher
Xia B, Zhong R, Wu W, Luo C, Meng Q, Gao Q, et al. Mucin O-glycan-microbiota axis orchestrates gut homeostasis in a diarrheal pig model. Microbiome. 2022;10:139 pubmed publisher
Wang P, Ren Q, Shi M, Liu Y, Bai H, Chang Y. Overexpression of Mitochondrial Ferritin Enhances Blood-Brain Barrier Integrity Following Ischemic Stroke in Mice by Maintaining Iron Homeostasis in Endothelial Cells. Antioxidants (Basel). 2022;11: pubmed publisher
Kim J, Lee E, Seo J, Lee Y, Ahn Y, Park S, et al. Nephrin expression in human epidermal keratinocytes and its implication in poor wound closure. FASEB J. 2022;36:e22424 pubmed publisher
Zhou M, Zhang Y, Ding H, Wu W, Cai W, Wang Y, et al. Diprotin A TFA Exerts Neurovascular Protection in Ischemic Cerebral Stroke. Front Neurosci. 2022;16:861059 pubmed publisher
Ma X, Jin H, Chu X, Dai W, Tang W, Zhu J, et al. The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal Sepsis. Front Microbiol. 2022;13:802409 pubmed publisher
Bastos R, Simpl xed cio Filho A, S xe1 vio Silva C, Oliveira L, Cruz G, Sousa E, et al. Fecal Microbiota Transplant in a Pre-Clinical Model of Type 2 Diabetes Mellitus, Obesity and Diabetic Kidney Disease. Int J Mol Sci. 2022;23: pubmed publisher
Zhao X, Zheng S, Wei S, Tian Q, Tao Y, Bo R, et al. The protective effect and potential mechanisms of eugenol against Salmonella in vivo and in vitro. Poult Sci. 2022;101:101801 pubmed publisher
McGrath C, Laveckis E, Bell A, Crost E, Juge N, Sch xfc ller S. Development of a novel human intestinal model to elucidate the effect of anaerobic commensals on Escherichia coli infection. Dis Model Mech. 2022;15: pubmed publisher
Chen L, Sutharsan R, Lee J, Cruz E, Asnicar B, Palliyaguru T, et al. Claudin-5 binder enhances focused ultrasound-mediated opening in an in vitro blood-brain barrier model. Theranostics. 2022;12:1952-1970 pubmed publisher
Zou P, Xiao Y, Deng Q, Shi Y, You R, Pi Z, et al. Occludin Promotes Adhesion of CD8+ T Cells and Melanocytes in Vitiligo via the HIF-1α Signaling Pathway. Oxid Med Cell Longev. 2022;2022:6732972 pubmed publisher
Byts N, Sharma S, Malm T, Kaakinen M, Korhonen P, Jaakkonen L, et al. Inactivation of mouse transmembrane prolyl 4-hydroxylase increases blood brain barrier permeability and ischemia-induced cerebral neuroinflammation. J Biol Chem. 2022;298:101721 pubmed publisher
Xia B, Wu W, Fang W, Wen X, Xie J, Zhang H. Heat stress-induced mucosal barrier dysfunction is potentially associated with gut microbiota dysbiosis in pigs. Anim Nutr. 2022;8:289-299 pubmed publisher
Yang Y, Fan X, Ji Y, Li J, Dai Z, Wu Z. Glycine represses endoplasmic reticulum stress-related apoptosis and improves intestinal barrier by activating mammalian target of rapamycin complex 1 signaling. Anim Nutr. 2022;8:1-9 pubmed publisher
Zhang X, Jiao X, Wu D, Chen F, Ding Z, Wang Y, et al. Benzophenone-3 breaches mouse Sertoli cell barrier and alters F-actin organization without evoking apoptosis. Environ Toxicol. 2022;37:28-40 pubmed publisher
Seo H, Seong H, Kim G, Jo Y, Cheon S, Song Y, et al. Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence. Front Microbiol. 2021;12:760476 pubmed publisher
Chataigner M, Lucas C, Di Miceli M, Pallet V, Layé S, Mehaignerie A, et al. Dietary Fish Hydrolysate Improves Memory Performance Through Microglial Signature Remodeling During Aging. Front Nutr. 2021;8:750292 pubmed publisher
Xu X, Gao W, Li L, Hao J, Yang B, Wang T, et al. Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway. J Neuroinflammation. 2021;18:119 pubmed publisher
Hasegawa T, Mizugaki A, Inoue Y, Kato H, Murakami H. Cystine reduces tight junction permeability and intestinal inflammation induced by oxidative stress in Caco-2 cells. Amino Acids. 2021;53:1021-1032 pubmed publisher
Chioccarelli T, Migliaccio M, Suglia A, Manfrevola F, Porreca V, Diano N, et al. Characterization of Estrogenic Activity and Site-Specific Accumulation of Bisphenol-A in Epididymal Fat Pad: Interfering Effects on the Endocannabinoid System and Temporal Progression of Germ Cells. Int J Mol Sci. 2021;22: pubmed publisher
McGrath C, Sch xfc ller S. Determining Shiga Toxin-Producing Escherichia coli Interactions with Human Intestinal Epithelium in a Microaerobic Vertical Diffusion Chamber. Methods Mol Biol. 2021;2291:273-283 pubmed publisher
Jia H, Zhang Y, Si X, Jin Y, Jiang D, Dai Z, et al. Quercetin Alleviates Oxidative Damage by Activating Nuclear Factor Erythroid 2-Related Factor 2 Signaling in Porcine Enterocytes. Nutrients. 2021;13: pubmed publisher
Scalisi J, Balau B, Deneyer L, Bouchat J, Gilloteaux J, Nicaise C. Blood-brain barrier permeability towards small and large tracers in a mouse model of osmotic demyelination syndrome. Neurosci Lett. 2021;746:135665 pubmed publisher
Salman M, Marsh G, Kusters I, Delince M, Di Caprio G, Upadhyayula S, et al. Design and Validation of a Human Brain Endothelial Microvessel-on-a-Chip Open Microfluidic Model Enabling Advanced Optical Imaging. Front Bioeng Biotechnol. 2020;8:573775 pubmed publisher
Hempt C, Kaiser J, Scholder O, Buerki Thurnherr T, Hofmann H, Rippl A, et al. The impact of synthetic amorphous silica (E 551) on differentiated Caco-2 cells, a model for the human intestinal epithelium. Toxicol In Vitro. 2020;67:104903 pubmed publisher
Johnson A, Harkema J, Nelson A, Dickinson J, Kalil J, Duryee M, et al. MyD88 regulates a prolonged adaptation response to environmental dust exposure-induced lung disease. Respir Res. 2020;21:97 pubmed publisher
Ducray H, Globa L, Pustovyy O, Roberts M, Rudisill M, Vodyanoy V, et al. Prevention of excessive exercise-induced adverse effects in rats with Bacillus subtilis BSB3. J Appl Microbiol. 2020;128:1163-1178 pubmed publisher
Sayoc Becerra A, Krishnan M, Fan S, Jimenez J, HERNANDEZ R, Gibson K, et al. The JAK-Inhibitor Tofacitinib Rescues Human Intestinal Epithelial Cells and Colonoids from Cytokine-Induced Barrier Dysfunction. Inflamm Bowel Dis. 2020;26:407-422 pubmed publisher
Zhou Y, Huang F, Liu Y, Li D, Zhou Y, Shen L, et al. TGF-β1 relieves epithelial-mesenchymal transition reduction in hypospadias induced by DEHP in rats. Pediatr Res. 2020;87:639-646 pubmed publisher
Dom xed nguez Salazar E, Hurtado Alvarado G, Medina Flores F, Dorantes J, Gonz xe1 lez Flores O, Contis Montes de Oca A, et al. Chronic sleep loss disrupts blood-testis and blood-epididymis barriers, and reduces male fertility. J Sleep Res. 2020;29:e12907 pubmed publisher
Gunasekaran A, Eckert J, Burge K, Zheng W, Yu Z, Kessler S, et al. Hyaluronan 35 kDa enhances epithelial barrier function and protects against the development of murine necrotizing enterocolitis. Pediatr Res. 2020;87:1177-1184 pubmed publisher
Noval Rivas M, Wakita D, Franklin M, Carvalho T, Abolhesn A, Gomez A, et al. Intestinal Permeability and IgA Provoke Immune Vasculitis Linked to Cardiovascular Inflammation. Immunity. 2019;51:508-521.e6 pubmed publisher
Ducray H, Globa L, Pustovyy O, Morrison E, Vodyanoy V, Sorokulova I. Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats. J Appl Microbiol. 2019;127:1192-1206 pubmed publisher
Zhou Z, Bian C, Luo Z, Guille C, Ogunrinde E, Wu J, et al. Progesterone decreases gut permeability through upregulating occludin expression in primary human gut tissues and Caco-2 cells. Sci Rep. 2019;9:8367 pubmed publisher
Oztas E, Yılmaz T, Guzel E, Sezer Z, Okyar A, Ozhan G. Gliclazide alone or in combination with atorvastatin ameliorated reproductive damage in streptozotocin-induced type 2 diabetic male rats. Saudi Pharm J. 2019;27:422-431 pubmed publisher
Chen L, Li H, Li J, Chen Y, Yang Y. Lactobacillus rhamnosus GG treatment improves intestinal permeability and modulates microbiota dysbiosis in an experimental model of sepsis. Int J Mol Med. 2019;43:1139-1148 pubmed publisher
Peng J, Leng J, Tian H, Yang T, Fang Y, Feng Q, et al. Geniposide and Chlorogenic Acid Combination Ameliorates Non-alcoholic Steatohepatitis Involving the Protection on the Gut Barrier Function in Mouse Induced by High-Fat Diet. Front Pharmacol. 2018;9:1399 pubmed publisher
Jin X, Liao Y, Tan X, Wang G, Zhao F, Jin Y. Involvement of CYP2E1 in the Course of Brain Edema Induced by Subacute Poisoning With 1,2-Dichloroethane in Mice. Front Pharmacol. 2018;9:1317 pubmed publisher
Janardhan K, Jensen H, Clayton N, Herbert R. Immunohistochemistry in Investigative and Toxicologic Pathology. Toxicol Pathol. 2018;46:488-510 pubmed publisher
Bilal S, Jaggi S, Janosevic D, Shah N, Teymour S, Voronina A, et al. ZO-1 protein is required for hydrogen peroxide to increase MDCK cell paracellular permeability in an ERK 1/2-dependent manner. Am J Physiol Cell Physiol. 2018;315:C422-C431 pubmed publisher
Allam O, Samarani S, Mehraj V, Jenabian M, Tremblay C, Routy J, et al. HIV induces production of IL-18 from intestinal epithelial cells that increases intestinal permeability and microbial translocation. PLoS ONE. 2018;13:e0194185 pubmed publisher
Menard C, Pfau M, Hodes G, Kana V, Wang V, Bouchard S, et al. Social stress induces neurovascular pathology promoting depression. Nat Neurosci. 2017;20:1752-1760 pubmed publisher
Lee J, Lee H, Kim T, Kim M, Park Y, Kim J, et al. Obesogenic diet-induced gut barrier dysfunction and pathobiont expansion aggravate experimental colitis. PLoS ONE. 2017;12:e0187515 pubmed publisher
DeStefano J, Xu Z, Williams A, Yimam N, Searson P. Effect of shear stress on iPSC-derived human brain microvascular endothelial cells (dhBMECs). Fluids Barriers CNS. 2017;14:20 pubmed publisher
Leclercq S, Mian F, Stanisz A, Bindels L, Cambier E, Ben Amram H, et al. Low-dose penicillin in early life induces long-term changes in murine gut microbiota, brain cytokines and behavior. Nat Commun. 2017;8:15062 pubmed publisher
Atallah A, Mhaouty Kodja S, Grange Messent V. Chronic depletion of gonadal testosterone leads to blood-brain barrier dysfunction and inflammation in male mice. J Cereb Blood Flow Metab. 2017;37:3161-3175 pubmed publisher
Lee J, Choi H, Na W, Ju B, Yune T. 17β-estradiol inhibits MMP-9 and SUR1/TrpM4 expression and activation and thereby attenuates BSCB disruption/hemorrhage after spinal cord injury in male rats. Endocrinology. 2015;156:1838-50 pubmed publisher
Sohet F, Lin C, Munji R, Lee S, Ruderisch N, Soung A, et al. LSR/angulin-1 is a tricellular tight junction protein involved in blood-brain barrier formation. J Cell Biol. 2015;208:703-11 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Occludin Polyclonal Antibody
Catalog # :
40-4700
Quantity :
100 µg
Price :
US 450
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Canine, Human, Mouse, Rat
Applications :
Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 6 µg/mL, Western Blot: 1-3 µg/mL
Species :
Canine, Human, Mouse, Rat
Isotype :
IgG
Storage :
-20°C
Description :
Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.
Immunogen :
Synthetic peptide derived from the C-terminal region of the human occludin protein
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 6 µg/mL, Western Blot: 1-3 µg/mL
Aliases :
AI503564; BLCPMG; Occludin; occludin 1B; Ocl; Ocln; phosphatase 1, regulatory subunit 115; PPP1R115; tight junction protein occludin; tight junction structural protein
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA