HIF1A Monoclonal Antibody (H1alpha67)
Invitrogen
catalog: MA1-16504

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dogs, bovine, pigs , cat, domestic sheep, Xenopus laevis, domestic rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay
citations: 69

Western Blot analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts.

Immunohistochemical staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.
quantity: 100 uL
price: US 496.00
to the supplier

HIF1A Monoclonal Antibody (H1alpha67)
Invitrogen
catalog: MA1-16511

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dogs, bovine, pigs , domestic sheep, domestic rabbit
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 53

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-16511.

Immunoprecipitation of HIF-1 alpha; Heavy and light chains are also detected.

Western Blot detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.
quantity: 100 uL
price: US 480.00
to the supplier

HIF1A Monoclonal Antibody (H1alpha67), Biotin
Invitrogen
catalog: MA1-46457

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dogs, bovine, pigs , domestic sheep, domestic rabbit
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 39

Immunoprecipitation of HIF-1alpha using MA1-46457; Heavy and light chains are also detected.

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-46457.

On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.
quantity: 100 uL
price: US 486.00
to the supplier

HIF1A Monoclonal Antibody (mgc3)
Invitrogen
catalog: MA1-516

mouse monoclonal (mgc3)
reactivity: human, mouse, rat, bovine, pigs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, immunohistochemistry - paraffin section, blocking or activating experiments
citations: 44

Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in Hela Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in U251 Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing HIF-1 alpha (MA1-516) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100 uL
price: US 562.00
to the supplier

HIF1A Polyclonal Antibody
Invitrogen
catalog: PA1-16601

domestic rabbit polyclonal
reactivity: domestic goat, Chinese hamsters, human, mouse, rat, dogs, pigs , domestic rabbit
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 32

Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human placenta villi tissue section using PA1-16601.

Detection of HIF-1 Alpha (green) in RCC4 cells using PA1-16601. Nuclei (Blue) were counterstained using Hoechst 33258.

Western blot analysis of HIF-1 alpha was performed by loading 50 ug of Cos7 cell lysates under hypoxic (Lane 1: CoCl2 treated) or untreated (Lane 2) conditions onto a gel and then transferring blotted to membrane. Blot was probed with a rabbit polyclonal antibody recognizing HIF-1 alpha (Product # PA1-16601) and chemiluminescent deterction performed using ECL.
quantity: 100 uL
price: US 494.00
to the supplier

HIF1A Monoclonal Antibody (H1alpha67), DyLight 650
Invitrogen
catalog: MA5-16008

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dogs, bovine, pigs , cat, domestic sheep, Xenopus laevis, domestic rabbit
conjugate: DyLight 650
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 35

quantity: 100 uL
price: US 491.00
to the supplier

HIF1A Monoclonal Antibody (H1alpha67), DyLight 550
Invitrogen
catalog: MA5-16010

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dogs, bovine, pigs , cat, domestic sheep, Xenopus laevis, domestic rabbit
conjugate: DyLight 550
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 34

quantity: 100 uL
price: US 486.00
to the supplier

HIF1A Monoclonal Antibody (H1alpha67), DyLight 488
Invitrogen
catalog: MA5-16009

mouse monoclonal (H1alpha67)
reactivity: human, mouse, rat, dogs, bovine, pigs , cat, domestic sheep, Xenopus laevis, domestic rabbit
conjugate: DyLight488
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 35

quantity: 100 uL
price: US 486.00
to the supplier

HIF1A Polyclonal Antibody
Invitrogen
catalog: PA1-16627

domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine, pigs
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western Blot detection of HIF-1 alpha in Cobalt Chloride treated/untreated Cos-7 nuclear extracts using PA1-16627.
quantity: 100 uL
price: US 538.00
to the supplier

HIF1A Polyclonal Antibody, DyLight 488
Invitrogen
catalog: PA5-22778

domestic rabbit polyclonal
reactivity: Chinese hamsters, domestic goat, human, mouse, rat, dogs, pigs , domestic rabbit
conjugate: DyLight488
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100 uL
price: US 486.00
to the supplier

HIF1A Polyclonal Antibody, HRP
Invitrogen
catalog: PA5-22779

domestic rabbit polyclonal
reactivity: Chinese hamsters, domestic goat, human, mouse, rat, dogs, pigs , domestic rabbit
conjugate: HRP
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100 uL
price: US 464.00
to the supplier

HIF1A Polyclonal Antibody, DyLight 550
Invitrogen
catalog: PA5-22780

domestic rabbit polyclonal
reactivity: Chinese hamsters, domestic goat, human, mouse, rat, dogs, pigs , domestic rabbit
conjugate: DyLight 550
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100 uL
price: US 480.00
to the supplier