FAK Monoclonal Antibody (ZF002) | Invitrogen 39-6500 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

FAK Monoclonal Antibody (ZF002)

catalog :

39-6500

quantity :

100 µg

price :

US 500

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ZF002

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 6a	Mondal B, Jin H, Kallappagoudar S, Sedkov Y, Martinez T, Sentmanat M, et al. The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. elife. 2020;9: pubmed publisher
western blot; human; 1:1000	Li H, Song H, Luo J, Liang J, Zhao S, Su R. Knockdown of glucose-regulated protein 78 decreases the invasion, metalloproteinase expression and ECM degradation in hepatocellular carcinoma cells. J Exp Clin Cancer Res. 2012;31:39 pubmed publisher
	Biber J, Sullivan A, Brazzo J, Heo Y, Tumenbayar B, Krajnik A, et al. Survivin as a mediator of stiffness-induced cell cycle progression and proliferation of vascular smooth muscle cells. APL Bioeng. 2023;7:046108 pubmed publisher
	Chen J, Zhu Y, Zhao D, Zhang L, Zhang J, Xiao Y, et al. Co-targeting FAK and Gli1 inhibits the tumor-associated macrophages-released CCL22-mediated esophageal squamous cell carcinoma malignancy. MedComm (2020). 2023;4:e381 pubmed publisher
	Brazzo J, Biber J, Nimmer E, Heo Y, Ying L, Zhao R, et al. Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression and cell proliferation. J Cell Sci. 2021;134: pubmed publisher
	Magdaleno C, Dixon L, Rajasekaran N, Varadaraj A. HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl2. Sci Rep. 2020;10:18560 pubmed publisher
	Bilodeau P, Jacobsen D, Law Vinh D, Lee J. Phosphatidylinositol 4-kinase III beta regulates cell shape, migration and Focal Adhesion number. Mol Biol Cell. 2020;:mbcE19110600 pubmed publisher
	Cutiongco M, Jensen B, Reynolds P, Gadegaard N. Predicting gene expression using morphological cell responses to nanotopography. Nat Commun. 2020;11:1384 pubmed publisher
	Tseng C, Lai Y, Kuo T, Su J, Sung P, Feng C, et al. Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells. Mar Drugs. 2019;17: pubmed publisher
	Wang X, Tang P, Guo F, Zhang M, Yan Y, Huang M, et al. mDia1 and Cdc42 Regulate Activin B-Induced Migration of Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells. 2019;37:150-162 pubmed publisher
	Fistonich C, Zehentmeier S, Bednarski J, Miao R, Schjerven H, Sleckman B, et al. Cell circuits between B cell progenitors and IL-7+ mesenchymal progenitor cells control B cell development. J Exp Med. 2018;215:2586-2599 pubmed publisher

product information

Product Type :

Antibody

Product Name :

FAK Monoclonal Antibody (ZF002)

Catalog # :

39-6500

Quantity :

100 µg

Price :

US 500

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 0.1-1 µg/mL, Immunocytochemistry: 1-2 µg/mL, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1 µg/mL

Species :

Human, Mouse, Rat

Clone :

ZF002

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

Immunogen :

Recombinant protein derived from the N-terminal region of the mouse and rat FAK-1 proteins

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1 µg/mL, Immunocytochemistry: 1-2 µg/mL, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1 µg/mL

Aliases :

CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK

more info or order :

Invitrogen product webpage