Phospho-p27 Kip1 (Thr187) Monoclonal Antibody (2B10B7) | Invitrogen 37-9700 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-p27 Kip1 (Thr187) Monoclonal Antibody (2B10B7)

catalog :

37-9700

quantity :

100 µg

price :

US 500.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

2B10B7

reactivity :

human

application :

western blot, ELISA

more info or order :

Invitrogen product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig s1	Vosper J, Masuccio A, Kullmann M, Ploner C, Geley S, Hengst L. Statin-induced depletion of geranylgeranyl pyrophosphate inhibits cell proliferation by a novel pathway of Skp2 degradation. Oncotarget. 2015;6:2889-902 pubmed
western blot; human	Yanagi T, Krajewska M, Matsuzawa S, Reed J. PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells. Cancer Res. 2014;74:5795-807 pubmed publisher
	Li W, Kotoshiba S, Berthet C, Hilton M, Kaldis P. Rb/Cdk2/Cdk4 triple mutant mice elicit an alternative mechanism for regulation of the G1/S transition. Proc Natl Acad Sci U S A. 2009;106:486-91 pubmed publisher
	Chen Q, Xie W, Kuhn D, Voorhees P, Lopez Girona A, Mendy D, et al. Targeting the p27 E3 ligase SCF(Skp2) results in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy. Blood. 2008;111:4690-9 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-p27 Kip1 (Thr187) Monoclonal Antibody (2B10B7)

Catalog # :

37-9700

Quantity :

100 µg

Price :

US 500.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Western Blot: 1-3 µg/mL

Species :

Human

Clone :

2B10B7

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Immunogen :

Synthetic peptide encompassing the phosphorylated Thr187 residue of human p27

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Western Blot: 1-3 µg/mL

Aliases :

AA408329; AI843786; Cdki1b; CDKN1B; CDKN4; CDN1B; cyclin dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B (p27 Kip1); Cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; cyclin-dependent kinase inhibitor p27/Kip1; KIP1; MEN1B; MEN4; p27; P27 CDKN1B; p27(KIP1); p27Kip1

more info or order :

Invitrogen product webpage