PLK1 Monoclonal Antibody (35-206) | Invitrogen 37-7000 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PLK1 Monoclonal Antibody (35-206)

catalog :

37-7000

quantity :

100 µg

price :

US 500

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

35-206

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 22

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:2000; loading ...; fig 1a	Yamanishi E, Hasegawa K, Fujita K, Ichinose S, Yagishita S, Murata M, et al. A novel form of necrosis, TRIAD, occurs in human Huntington's disease. Acta Neuropathol Commun. 2017;5:19 pubmed publisher
western blot; human; fig 1	Jayashree B, Srimany A, Jayaraman S, Bhutra A, Janakiraman N, Chitipothu S, et al. Monitoring of changes in lipid profiles during PLK1 knockdown in cancer cells using DESI MS. Anal Bioanal Chem. 2016;408:5623-32 pubmed publisher
western blot; rat; 1:500; fig 4	Mao Y, Tamura T, Yuki Y, Abe D, Tamada Y, Imoto S, et al. The hnRNP-Htt axis regulates necrotic cell death induced by transcriptional repression through impaired RNA splicing. Cell Death Dis. 2016;7:e2207 pubmed publisher
flow cytometry; human; fig 2	Hasvold G, Lund Andersen C, Lando M, Patzke S, Hauge S, Suo Z, et al. Hypoxia-induced alterations of G2 checkpoint regulators. Mol Oncol. 2016;10:764-73 pubmed publisher
western blot; human; fig 5	Saini P, Li Y, Dobbelstein M. Wee1 is required to sustain ATR/Chk1 signaling upon replicative stress. Oncotarget. 2015;6:13072-87 pubmed
western blot; human; 1:100; fig 7	Fan Y, Zhao H, Liu J, Tan T, Ding T, Li R, et al. Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes. Sci Rep. 2015;5:8192 pubmed publisher
western blot; human western blot; mouse	Vassilopoulos A, Tominaga Y, Kim H, Lahusen T, Li B, Yu H, et al. WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis. Oncogene. 2015;34:3023-35 pubmed publisher
western blot; human; 1:2500	Akiyama M, Izumi H, Wang K, Yamaguchi T, Kuma A, Kitamura N, et al. Hypersensitivity to aurora kinase inhibitors in cells resistant against platinum- containing anticancer agents. Anticancer Agents Med Chem. 2014;14:1042-50 pubmed
western blot; human; fig s2	Nakamura T, Saito H, Takekawa M. SAPK pathways and p53 cooperatively regulate PLK4 activity and centrosome integrity under stress. Nat Commun. 2013;4:1775 pubmed publisher
western blot; human; 1:200; fig 5	Landsverk K, Patzke S, Rein I, Stokke C, Lyng H, De Angelis P, et al. Three independent mechanisms for arrest in G2 after ionizing radiation. Cell Cycle. 2011;10:819-29 pubmed
immunoprecipitation; human immunocytochemistry; human western blot; human	Fabbro M, Zhou B, Takahashi M, Sarcevic B, Lal P, Graham M, et al. Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis. Dev Cell. 2005;9:477-88 pubmed
	Li Y, Li Y, Zhang X, Duan X, Feng H, Yu Z, et al. A novel association of pyroptosis-related gene signature with the prognosis of hepatocellular carcinoma. Front Oncol. 2022;12:986827 pubmed publisher
	Kamranvar S, Gupta D, Wasberg A, Liu L, Roig J, Johansson S. Integrin-Mediated Adhesion Promotes Centrosome Separation in Early Mitosis. Cells. 2022;11: pubmed publisher
	Xie G, Zhou Y, Du M, Wang Q, Yi J, Zhang X. Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines. STAR Protoc. 2021;2:100495 pubmed publisher
	Yu R, Wu H, Ismail H, Du S, Cao J, Wang J, et al. Methylation of PLK1 by SET7/9 ensures accurate kinetochore-microtubule dynamics. J Mol Cell Biol. 2020;12:462-476 pubmed publisher
	Feitosa W, Hwang K, Morris P. Temporal and SUMO-specific SUMOylation contribute to the dynamics of Polo-like kinase 1 (PLK1) and spindle integrity during mouse oocyte meiosis. Dev Biol. 2018;434:278-291 pubmed publisher
	Haibara H, Yamazaki R, Nishiyama Y, Ono M, Kobayashi T, Hokkyo Itagaki A, et al. YPC-21661 and YPC-22026, novel small molecules, inhibit ZNF143 activity in vitro and in vivo. Cancer Sci. 2017;108:1042-1048 pubmed publisher
	Mao Y, Chen X, Xu M, Fujita K, Motoki K, Sasabe T, et al. Targeting TEAD/YAP-transcription-dependent necrosis, TRIAD, ameliorates Huntington's disease pathology. Hum Mol Genet. 2016;25:4749-4770 pubmed publisher
	Clift D, Schuh M. A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes. Nat Commun. 2015;6:7217 pubmed publisher
	Izumi H, Yasuniwa Y, Akiyama M, Yamaguchi T, Kuma A, Kitamura N, et al. Forced Expression of ZNF143 Restrains Cancer Cell Growth. Cancers (Basel). 2011;3:3909-20 pubmed publisher
	Zhang B, Huang B, Guan H, Zhang S, Xu Q, He X, et al. Proteomic profiling revealed the functional networks associated with mitotic catastrophe of HepG2 hepatoma cells induced by 6-bromine-5-hydroxy-4-methoxybenzaldehyde. Toxicol Appl Pharmacol. 2011;252:307-17 pubmed publisher
	Wang Z, Udeshi N, Slawson C, Compton P, Sakabe K, Cheung W, et al. Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis. Sci Signal. 2010;3:ra2 pubmed publisher

product information

Product Type :

Antibody

Product Name :

PLK1 Monoclonal Antibody (35-206)

Catalog # :

37-7000

Quantity :

100 µg

Price :

US 500

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL

Species :

Human, Mouse, Rat

Clone :

35-206

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

Immunogen :

Full-length human Plk1 (polo-like kinase 1)

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 µg/mL

Aliases :

cell cycle regulated protein kinase; Plk; PLK 1; Plk1; PLK-1; plk1.S; plx1; polo (Drosophia)-like kinase; polo like kinase; polo like kinase 1; polo like kinase 1 S homeolog; polo-like kinase; polo-like kinase 1; polo-like kinase homolog; polo-like protein kinase; serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14; Unknown (protein for MGC:133927); XELAEV_18047692mg

more info or order :

Invitrogen product webpage