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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Claudin 7 Monoclonal Antibody (5D10F3)
catalog :
37-4800
quantity :
100 µg
price :
US 450
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5D10F3
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 19
Published Application/Species/Sample/DilutionReference
  • western blot; human
Watari A, Hashegawa M, Yagi K, Kondoh M. Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers. Eur J Pharm Biopharm. 2015;89:232-8 pubmed publisher
  • immunohistochemistry; human; 1:50; fig 1
Ma F, Ding X, Fan Y, Ying J, Zheng S, Lu N, et al. A CLDN1-negative phenotype predicts poor prognosis in triple-negative breast cancer. PLoS ONE. 2014;9:e112765 pubmed publisher
  • western blot; human; fig 11
Kinoshita S, Kawasaki S, Kitazawa K, Shinomiya K. Establishment of a human conjunctival epithelial cell line lacking the functional TACSTD2 gene (an American Ophthalmological Society thesis). Trans Am Ophthalmol Soc. 2012;110:166-77 pubmed
  • immunohistochemistry - paraffin section; human; 1:1000; fig 1
Victoria H, Henrik E, Lennart B, Lennart F. Claudin 1 and Claudin 7 Gene Polymorphisms and Protein Derangement are Unrelated to the Growth Pattern and Tumor Volume of Colon Carcinoma. Int J Biomed Sci. 2010;6:96-102 pubmed
  • western blot; human; 1:500; fig S4
Krishnan M, Lapierre L, Knowles B, Goldenring J. Rab25 regulates integrin expression in polarized colonic epithelial cells. Mol Biol Cell. 2013;24:818-31 pubmed publisher
  • immunohistochemistry; human; fig 4
Ohe C, Kuroda N, Takasu K, Senzaki H, Shikata N, Yamaguchi T, et al. Utility of immunohistochemical analysis of KAI1, epithelial-specific antigen, and epithelial-related antigen for distinction of chromophobe renal cell carcinoma, an eosinophilic variant from renal oncocytoma. Med Mol Morphol. 2012;45:98-104 pubmed publisher
  • immunohistochemistry; human; fig 3
Lisch W, Bron A, Munier F, Schorderet D, Tiab L, Lange C, et al. Franceschetti hereditary recurrent corneal erosion. Am J Ophthalmol. 2012;153:1073-81.e4 pubmed publisher
  • immunocytochemistry; human; fig 5
Comamala M, Pinard M, Theriault C, Matte I, Albert A, Boivin M, et al. Downregulation of cell surface CA125/MUC16 induces epithelial-to-mesenchymal transition and restores EGFR signalling in NIH:OVCAR3 ovarian carcinoma cells. Br J Cancer. 2011;104:989-99 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:400; fig 3
Carvalho J, Wasco M, Kunju L, Thomas D, Shah R. Cluster analysis of immunohistochemical profiles delineates CK7, vimentin, S100A1 and C-kit (CD117) as an optimal panel in the differential diagnosis of renal oncocytoma from its mimics. Histopathology. 2011;58:169-79 pubmed publisher
  • immunocytochemistry; human; fig 5
  • immunohistochemistry; human; fig 2
  • western blot; human; fig 2
Nakatsukasa M, Kawasaki S, Yamasaki K, Fukuoka H, Matsuda A, Tsujikawa M, et al. Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy. Am J Pathol. 2010;177:1344-55 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; fig 4a
  • immunocytochemistry; human; fig 4h
Tassi R, Bignotti E, Falchetti M, Ravanini M, Calza S, Ravaggi A, et al. Claudin-7 expression in human epithelial ovarian cancer. Int J Gynecol Cancer. 2008;18:1262-71 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 1
Sauer T, Pedersen M, Ebeltoft K, Naess O. Reduced expression of Claudin-7 in fine needle aspirates from breast carcinomas correlate with grading and metastatic disease. Cytopathology. 2005;16:193-8 pubmed
Hollis P, Mobley R, Bhuju J, Abell A, Sutter C, Sutter T. CYP1B1 Augments the Mesenchymal, Claudin-Low, and Chemoresistant Phenotypes of Triple-Negative Breast Cancer Cells. Int J Mol Sci. 2022;23: pubmed publisher
Koumangoye R, Penny P, Delpire E. Loss of NKCC1 function increases epithelial tight junction permeability by upregulating claudin-2 expression. Am J Physiol Cell Physiol. 2022;323:C1251-C1263 pubmed publisher
Naser A, Guiler W, Lu Q, Chen Y. Nanoarchitecture and molecular interactions of epithelial cell junction proteins revealed by super-resolution microscopy. Ann N Y Acad Sci. 2022;1516:175-187 pubmed publisher
Ozawa M, Hiver S, Yamamoto T, Shibata T, Upadhyayula S, Mimori Kiyosue Y, et al. Adherens junction regulates cryptic lamellipodia formation for epithelial cell migration. J Cell Biol. 2020;219: pubmed publisher
Ahmad R, Rah B, Bastola D, Dhawan P, Singh A. Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching. Sci Rep. 2017;7:5125 pubmed publisher
Wu C, Feng X, Lu M, Morimura S, Udey M. Matriptase-mediated cleavage of EpCAM destabilizes claudins and dysregulates intestinal epithelial homeostasis. J Clin Invest. 2017;127:623-634 pubmed publisher
Kirk A, Campbell S, Bass P, Mason J, Collins J. Differential expression of claudin tight junction proteins in the human cortical nephron. Nephrol Dial Transplant. 2010;25:2107-19 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Claudin 7 Monoclonal Antibody (5D10F3)
Catalog # :
37-4800
Quantity :
100 µg
Price :
US 450
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
ELISA: Assay-dependent, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1:50-1:500, Immunoprecipitation: Assay-dependent, Western Blot: 2 µg/mL
Species :
Human, Mouse
Clone :
5D10F3
Isotype :
IgG2a, kappa
Storage :
-20°C
Description :
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.
Immunogen :
Synthetic peptide derived from the C-terminal region of the human claudin-7 protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1:50-1:500, Immunoprecipitation: Assay-dependent, Western Blot: 2 µg/mL
Aliases :
CEPTRL2; claudin 7; claudin-1; Claudin-7; Cldn7; CLDN-7; clostridium perfringens enterotoxin receptor-like 2; CPETRL2; Hs.84359
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA