Connexin 36 Monoclonal Antibody (1E5H5) | Invitrogen 37-4600 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Connexin 36 Monoclonal Antibody (1E5H5)

catalog :

37-4600

quantity :

100 µg

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E5H5

reactivity :

Seba's short-tailed bat, western mosquitofish, human, mouse, rat, domestic sheep

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation, immunohistochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 37

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; fig 1 immunoprecipitation; human; fig 5 immunocytochemistry; human; 2 ug/ml; fig 4 immunohistochemistry knockout validation; mouse; 3 ug/ml; fig 2	Li X, Olson C, Lu S, Nagy J. Association of connexin36 with zonula occludens-1 in HeLa cells, betaTC-3 cells, pancreas, and adrenal gland. Histochem Cell Biol. 2004;122:485-98 pubmed
immunohistochemistry; human; 1:100; loading ...; fig 1c western blot; human; 1:500; loading ...; fig 6b	Coyle D, Doyle B, Murphy J, O Donnell A, Gillick J, Puri P. Expression of connexin 26 and connexin 43 is reduced in Hirschsprung's disease. J Surg Res. 2016;206:242-251 pubmed publisher
immunocytochemistry; mouse; 1:500; fig 5 western blot; mouse; 1:500; fig 4	Meyer A, Tetenborg S, Greb H, Segelken J, Dorgau B, Weiler R, et al. Connexin30.2: In Vitro Interaction with Connexin36 in HeLa Cells and Expression in AII Amacrine Cells and Intrinsically Photosensitive Ganglion Cells in the Mouse Retina. Front Mol Neurosci. 2016;9:36 pubmed publisher
immunohistochemistry - frozen section; mouse	Bolte P, Herrling R, Dorgau B, Schultz K, Feigenspan A, Weiler R, et al. Expression and Localization of Connexins in the Outer Retina of the Mouse. J Mol Neurosci. 2016;58:178-92 pubmed publisher
immunohistochemistry; western mosquitofish; fig 8	Serrano Velez J, Rodriguez Alvarado M, Torres Vazquez I, Fraser S, Yasumura T, Vanderpool K, et al. Abundance of gap junctions at glutamatergic mixed synapses in adult Mosquitofish spinal cord neurons. Front Neural Circuits. 2014;8:66 pubmed publisher
western blot; domestic sheep	Sadowska G, Stonestreet B. Maternal treatment with glucocorticoids modulates gap junction protein expression in the ovine fetal brain. Neuroscience. 2014;275:248-58 pubmed publisher
immunocytochemistry; rat immunocytochemistry; mouse	Bautista W, Rash J, Vanderpool K, Yasumura T, Nagy J. Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus. Eur J Neurosci. 2014;39:757-70 pubmed publisher
immunocytochemistry; human; fig s2	Rash J, Curti S, Vanderpool K, Kamasawa N, Nannapaneni S, Palacios Prado N, et al. Molecular and functional asymmetry at a vertebrate electrical synapse. Neuron. 2013;79:957-69 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 3	Li D, Sekhon P, Barr K, Marquez Rosado L, Lampe P, Kidder G. Connexins and steroidogenesis in mouse Leydig cells. Can J Physiol Pharmacol. 2013;91:157-64 pubmed publisher
immunohistochemistry - frozen section; Seba's short-tailed bat; 1:100; fig 4	M ller B, Butz E, Peichl L, Haverkamp S. The rod pathway of the microbat retina has bistratified rod bipolar cells and tristratified AII amacrine cells. J Neurosci. 2013;33:1014-23 pubmed publisher
western blot; mouse; fig 1	Katti C, Butler R, Sekaran S. Diurnal and circadian regulation of connexin 36 transcript and protein in the mammalian retina. Invest Ophthalmol Vis Sci. 2013;54:821-9 pubmed publisher
western blot; rat; 1:250	Corthell J, Fadool D, Trombley P. Connexin and AMPA receptor expression changes over time in the rat olfactory bulb. Neuroscience. 2012;222:38-48 pubmed publisher
immunohistochemistry; rat	Hamzei Sichani F, Davidson K, Yasumura T, Janssen W, Wearne S, Hof P, et al. Mixed Electrical-Chemical Synapses in Adult Rat Hippocampus are Primarily Glutamatergic and Coupled by Connexin-36. Front Neuroanat. 2012;6:13 pubmed publisher
immunohistochemistry; mouse; 1:750; fig 1	Hilgen G, von Maltzahn J, Willecke K, Weiler R, Dedek K. Subcellular distribution of connexin45 in OFF bipolar cells of the mouse retina. J Comp Neurol. 2011;519:433-50 pubmed publisher
immunohistochemistry - frozen section; human; 1:100; fig 7	Liu W, Bostrom M, Kinnefors A, Rask Andersen H. Unique expression of connexins in the human cochlea. Hear Res. 2009;250:55-62 pubmed publisher
western blot; rat; fig 2	Yates C, Charlesworth A, Reese N, Ishida K, Skinner R, Garcia Rill E. Modafinil normalized hyperreflexia after spinal transection in adult rats. Spinal Cord. 2009;47:481-5 pubmed publisher
immunoprecipitation; rat	Yates C, Charlesworth A, Allen S, Reese N, Skinner R, Garcia Rill E. The onset of hyperreflexia in the rat following complete spinal cord transection. Spinal Cord. 2008;46:798-803 pubmed publisher
immunohistochemistry; mouse; 3 ug/ml	Ciolofan C, Lynn B, Wellershaus K, Willecke K, Nagy J. Spatial relationships of connexin36, connexin57 and zonula occludens-1 in the outer plexiform layer of mouse retina. Neuroscience. 2007;148:473-88 pubmed
immunohistochemistry; mouse; 10 ug/ml; fig 1E immunohistochemistry; rat; 10 ug/ml; fig 1C	Rash J, Olson C, Davidson K, Yasumura T, Kamasawa N, Nagy J. Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus. Neuroscience. 2007;147:938-56 pubmed
immunohistochemistry; mouse; 3 ug/ml; fig 4	Ciolofan C, Li X, Olson C, Kamasawa N, Gebhardt B, Yasumura T, et al. Association of connexin36 and zonula occludens-1 with zonula occludens-2 and the transcription factor zonula occludens-1-associated nucleic acid-binding protein at neuronal gap junctions in rodent retina. Neuroscience. 2006;140:433-51 pubmed
	Hilgen G. Connexin45 colocalization patterns in the plexiform layers of the developing mouse retina. J Anat. 2023;243:258-264 pubmed publisher
	Fadjukov J, Wienbar S, Hakanen S, Aho V, Vihinen Ranta M, Ihalainen T, et al. Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium. J Gen Physiol. 2022;154: pubmed publisher
	Lautz J, Tsegay K, Zhu Z, Gniffke E, Welsh J, Smith S. Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states. Cell Rep. 2021;37:110076 pubmed publisher
	Chen A, Azar S, Harris A, Brecha N, P xe9 rez de Sevilla M xfc ller L. PTEN Expression Regulates Gap Junction Connectivity in the Retina. Front Neuroanat. 2021;15:629244 pubmed publisher
	Harrison K, Chervenak A, Resnick S, Reifler A, Wong K. Amacrine Cells Forming Gap Junctions With Intrinsically Photosensitive Retinal Ganglion Cells: ipRGC Types, Neuromodulator Contents, and Connexin Isoform. Invest Ophthalmol Vis Sci. 2021;62:10 pubmed publisher
	Tetenborg S, Wang H, Nemitz L, Depping A, Espejo A, Aseervatham J, et al. Phosphorylation of Connexin36 near the C-terminus switches binding affinities for PDZ-domain and 14-3-3 proteins in vitro. Sci Rep. 2020;10:18378 pubmed publisher
	Zhang S, Lyuboslavsky P, Dixon J, Chrenek M, Sellers J, Hamm J, et al. Effects of Cone Connexin-36 Disruption on Light Adaptation and Circadian Regulation of the Photopic ERG. Invest Ophthalmol Vis Sci. 2020;61:24 pubmed publisher
	Yadav S, Tetenborg S, Dedek K. Gap Junctions in A8 Amacrine Cells Are Made of Connexin36 but Are Differently Regulated Than Gap Junctions in AII Amacrine Cells. Front Mol Neurosci. 2019;12:99 pubmed publisher
	Kobayakawa Y, Masaki K, Yamasaki R, Shiraishi W, Hayashida S, Hayashi S, et al. Downregulation of Neuronal and Dendritic Connexin36-Made Electrical Synapses Without Glutamatergic Axon Terminals in Spinal Anterior Horn Cells From the Early Stage of Amyotrophic Lateral Sclerosis. Front Neurosci. 2018;12:894 pubmed publisher
	Veruki M, Zhou Y, Castilho A, Morgans C, Hartveit E. Extrasynaptic NMDA Receptors on Rod Pathway Amacrine Cells: Molecular Composition, Activation, and Signaling. J Neurosci. 2019;39:627-650 pubmed publisher
	Tetenborg S, Yadav S, Hormuzdi S, Monyer H, Janssen Bienhold U, Dedek K. Differential Distribution of Retinal Ca2+/Calmodulin-Dependent Kinase II (CaMKII) Isoforms Indicates CaMKII-β and -δ as Specific Elements of Electrical Synapses Made of Connexin36 (Cx36). Front Mol Neurosci. 2017;10:425 pubmed publisher
	BrÃ¼ggen B, Meyer A, Boven F, Weiler R, Dedek K. Type 2 wide-field amacrine cells in TH::GFP mice show a homogenous synapse distribution and contact small ganglion cells. Eur J Neurosci. 2015;41:734-47 pubmed publisher
	Li X, Kamasawa N, Ciolofan C, Olson C, Lu S, Davidson K, et al. Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bihomotypic gap junctions, with scaffolding contributed by zonula occludens-1. J Neurosci. 2008;28:9769-89 pubmed publisher
	Yates C, Charlesworth A, Reese N, Skinner R, Garcia Rill E. The effects of passive exercise therapy initiated prior to or after the development of hyperreflexia following spinal transection. Exp Neurol. 2008;213:405-9 pubmed publisher
	Horowitz S, Stamper S, Simmons J. Neuronal connexin expression in the cochlear nucleus of big brown bats. Brain Res. 2008;1197:76-84 pubmed publisher
	Rash J, Davidson K, Kamasawa N, Yasumura T, Kamasawa M, Zhang C, et al. Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb. J Neurocytol. 2005;34:307-41 pubmed
	Li X, Olson C, Lu S, Kamasawa N, Yasumura T, Rash J, et al. Neuronal connexin36 association with zonula occludens-1 protein (ZO-1) in mouse brain and interaction with the first PDZ domain of ZO-1. Eur J Neurosci. 2004;19:2132-46 pubmed

product information

Product Type :

Antibody

Product Name :

Connexin 36 Monoclonal Antibody (1E5H5)

Catalog # :

37-4600

Quantity :

100 µg

Price :

US 450.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 2-3 µg/mL, Immunohistochemistry: 2-3 µg/mL

Species :

Human, Mouse

Clone :

1E5H5

Isotype :

IgG1

Storage :

-20°C

Description :

Connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons. Mutations in connexin-encoding genes can lead to functional and developmental abnormalities.

Immunogen :

Synthetic peptide derived from the C-terminal region of the rat and mouse Connexin36 proteins.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2-3 µg/mL, Immunohistochemistry: 2-3 µg/mL

Aliases :

connexin 36; connexin36; connexin-36; cx36; Gap junction alpha-9 protein; gap junction delta-2 protein; gap junction membrane channel protein alpha 9; gap junction protein delta 2; gap junction protein, alpha 9; gap junction protein, delta 2; gap junction protein, delta 2, 36kDa; GJA10; Gja9; GJD2

more info or order :

Invitrogen product webpage