This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
6x-His Tag Monoclonal Antibody (4A12E4)
catalog :
37-2900
quantity :
100 ug
price :
US 469.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4A12E4
application :
western blot, ELISA, immunocytochemistry
citations: 50
Reference
Sun M, Zhang C, Sui D, Yang C, Pyeon D, Huang X, et al. Rational Design and Synthesis of D-galactosyl Lysophospholipids as Selective Substrates and non-ATP-competitive Inhibitors of Phosphatidylinositol Phosphate Kinases. Chemistry. 2023;29:e202202083 pubmed publisher
Akkermans O, Delloye Bourgeois C, Peregrina C, Carrasquero Ordaz M, Kokolaki M, Berbeira Santana M, et al. GPC3-Unc5 receptor complex structure and role in cell migration. Cell. 2022;185:3931-3949.e26 pubmed publisher
Singh S, Dahiya S, Singh Y, Beeton K, Jain A, Sarkar R, et al. Robust anti-SARS-CoV2 single domain antibodies cross neutralize multiple viruses. iScience. 2022;25:104549 pubmed publisher
Hoff K, Aiken J, Gutierrez M, Franco S, Moore J. TUBA1A tubulinopathy mutants disrupt neuron morphogenesis and override XMAP215/Stu2 regulation of microtubule dynamics. elife. 2022;11: pubmed publisher
Xu C, Dinh V, Kruse K, Jeong H, Watson E, Adams S, et al. Induction of osteogenesis by bone-targeted Notch activation. elife. 2022;11: pubmed publisher
Romero L, Hattori T, Ali M, Ketavarapu G, Koide A, Park C, et al. High-valency Anti-CD99 Antibodies Toward the Treatment of T Cell Acute Lymphoblastic Leukemia. J Mol Biol. 2022;434:167402 pubmed publisher
Buscaglia G, Northington K, Aiken J, Hoff K, Bates E. Bridging the Gap: The Importance of TUBA1A α-Tubulin in Forming Midline Commissures. Front Cell Dev Biol. 2021;9:789438 pubmed publisher
Tada T, Zhou H, Dcosta B, Samanovic M, Mulligan M, Landau N. Partial resistance of SARS-CoV-2 Delta variants to vaccine-elicited antibodies and convalescent sera. iScience. 2021;24:103341 pubmed publisher
Hyun S, Imamura A, Ishida H, Piepenbrink K, Goldblum S, Lillehoj E. The sialidase NEU1 directly interacts with the juxtamembranous segment of the cytoplasmic domain of mucin-1 to inhibit downstream PI3K-Akt signaling. J Biol Chem. 2021;297:101337 pubmed publisher
McInnes J, Zhou L, Verstreken A. Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification. Bio Protoc. 2018;8:e3043 pubmed publisher
Patterson Orazem A, Qerqez A, Azouz L, Ma M, Hill S, Ku Y, et al. Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin. J Biol Chem. 2021;297:101067 pubmed publisher
Elfahmi -, Hapsari R, Chrysanthy T, Synthiarini V, Masduki F, Setiawan A, et al. Expression of Two Key Enzymes of Artemisinin Biosynthesis FPS and ADS genes in Saccharomyces cerevisiae. Adv Pharm Bull. 2021;11:181-187 pubmed publisher
Li Q, Li W, Xu K, Xing Y, Shi H, Jing Z, et al. PEG Linker Improves Antitumor Efficacy and Safety of Affibody-Based Drug Conjugates. Int J Mol Sci. 2021;22: pubmed publisher
Tada T, Fan C, Chen J, Kaur R, Stapleford K, Gristick H, et al. An ACE2 Microbody Containing a Single Immunoglobulin Fc Domain Is a Potent Inhibitor of SARS-CoV-2. Cell Rep. 2020;33:108528 pubmed publisher
Henzi A, Senatore A, Lakkaraju A, Scheckel C, M xfc hle J, Reimann R, et al. Soluble dimeric prion protein ligand activates Adgrg6 receptor but does not rescue early signs of demyelination in PrP-deficient mice. PLoS ONE. 2020;15:e0242137 pubmed publisher
Green R, Izac J, Naimi W, O Bier N, Breitschwerdt E, Marconi R, et al. Ehrlichia chaffeensis EplA Interaction With Host Cell Protein Disulfide Isomerase Promotes Infection. Front Cell Infect Microbiol. 2020;10:500 pubmed publisher
Rouaud F, Tessaro F, Aimaretti L, Scapozza L, Citi S. Cooperative binding of the tandem WW domains of PLEKHA7 to PDZD11 promotes conformation-dependent interaction with tetraspanin 33. J Biol Chem. 2020;295:9299-9312 pubmed publisher
del Toro D, Carrasquero Ordaz M, Chu A, Ruff T, Shahin M, Jackson V, et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell. 2020;180:323-339.e19 pubmed publisher
Kosolapova A, Belousov M, Sulatskaya A, Belousova M, Sulatsky M, Antonets K, et al. Two Novel Amyloid Proteins, RopA and RopB, from the Root Nodule Bacterium Rhizobium leguminosarum. Biomolecules. 2019;9: pubmed publisher
Farah C, Dunn T, Hastings M, Ferguson L, Gao C, Gong K, et al. A role for Numb in Protein kinase M (PKM)-mediated increase in surface AMPA receptors during facilitation in Aplysia. J Neurochem. 2019;150:366-384 pubmed publisher
Roudnický P, Vorel J, Ilgová J, Benovics M, Norek A, Jedličková L, et al. Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea). Parasite. 2018;25:61 pubmed publisher
Checa Rojas A, Delgadillo Silva L, Velasco Herrera M, Andrade Domínguez A, Gil J, Santillán O, et al. GSTM3 and GSTP1: novel players driving tumor progression in cervical cancer. Oncotarget. 2018;9:21696-21714 pubmed publisher
Vogl T, Stratis A, Wixler V, Voller T, Thurainayagam S, Jorch S, et al. Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation. J Clin Invest. 2018;128:1852-1866 pubmed publisher
McInnes J, Wierda K, Snellinx A, Bounti L, Wang Y, Stancu I, et al. Synaptogyrin-3 Mediates Presynaptic Dysfunction Induced by Tau. Neuron. 2018;97:823-835.e8 pubmed publisher
Morgan G, Burkhardt D, Kelly J, Powers E. Translation efficiency is maintained at elevated temperature in Escherichia coli. J Biol Chem. 2018;293:777-793 pubmed publisher
González Montoro A, Bigliani G, Valdez Taubas J. The shape of the transmembrane domain is a novel endocytosis signal for single-spanning membrane proteins. J Cell Sci. 2017;130:3829-3838 pubmed publisher
Huang S, Zhou A, Nguyen D, Zhang H, Benz E. Protein 4.1R Influences Myogenin Protein Stability and Skeletal Muscle Differentiation. J Biol Chem. 2016;291:25591-25607 pubmed
Breard E, Garnier A, Despres P, Blaise Boisseau S, Comtet L, Viarouge C, et al. Development of a Double-Antigen Microsphere Immunoassay for Simultaneous Group and Serotype Detection of Bluetongue Virus Antibodies. Transbound Emerg Dis. 2017;64:1837-1847 pubmed publisher
Humbert M, Hung M, Phillips R, Akoto C, Hill A, Tan W, et al. Vaccine Potential and Diversity of the Putative Cell Binding Factor (CBF, NMB0345/NEIS1825) Protein of Neisseria meningitidis. PLoS ONE. 2016;11:e0160403 pubmed publisher
Guo R, Guo W, Cao L, Liu H, Liu J, Xu H, et al. Fusion of an albumin-binding domain extends the half-life of immunotoxins. Int J Pharm. 2016;511:538-549 pubmed publisher
Selb R, Eckl Dorna J, Twaroch T, Lupinek C, Teufelberger A, Hofer G, et al. Critical and direct involvement of the CD23 stalk region in IgE binding. J Allergy Clin Immunol. 2017;139:281-289.e5 pubmed publisher
Guerrera D, Shah J, Vasileva E, Sluysmans S, Méan I, Jond L, et al. PLEKHA7 Recruits PDZD11 to Adherens Junctions to Stabilize Nectins. J Biol Chem. 2016;291:11016-29 pubmed publisher
Kim G, Luján R, Schwenk J, Kelley M, Aguado C, Watanabe M, et al. Membrane palmitoylated protein 2 is a synaptic scaffold protein required for synaptic SK2-containing channel function. elife. 2016;5: pubmed publisher
Wang J, Goetsch L, Tucker L, Zhang Q, Gonzalez A, Vaidya K, et al. Anti-c-Met monoclonal antibody ABT-700 breaks oncogene addiction in tumors with MET amplification. BMC Cancer. 2016;16:105 pubmed publisher
Cassady Cain R, Blackburn E, Alsarraf H, Dedic E, Bease A, Bottcher B, et al. Biophysical Characterization and Activity of Lymphostatin, a Multifunctional Virulence Factor of Attaching and Effacing Escherichia coli. J Biol Chem. 2016;291:5803-16 pubmed publisher
Niknejad A, Webster D, Bhave M. Production of bioactive wheat puroindoline proteins in Nicotiana benthamiana using a virus-based transient expression system. Protein Expr Purif. 2016;125:43-52 pubmed publisher
Lu Q, Schafer D, Adler P. The Drosophila planar polarity gene multiple wing hairs directly regulates the actin cytoskeleton. Development. 2015;142:2478-86 pubmed publisher
Wang Z, Wei M, Zhang H, Chen H, Germana S, Huang C, et al. Diphtheria-toxin based anti-human CCR4 immunotoxin for targeting human CCR4(+) cells in vivo. Mol Oncol. 2015;9:1458-70 pubmed publisher
Wei Z, Salmon R, Upton P, Morrell N, Li W. Regulation of bone morphogenetic protein 9 (BMP9) by redox-dependent proteolysis. J Biol Chem. 2014;289:31150-9 pubmed publisher
Peraino J, Zhang H, Rajasekera P, Wei M, Madsen J, Sachs D, et al. Diphtheria toxin-based bivalent human IL-2 fusion toxin with improved efficacy for targeting human CD25(+) cells. J Immunol Methods. 2014;405:57-66 pubmed publisher
Zhou F, Cui L, Huang Z, Tong S, Yu M, Liu R, et al. The expression pattern and histological distribution of sialyltransferases ST3Gal III in yellow chicken. Vet Res Commun. 2013;37:285-91 pubmed publisher
Peraino J, Schenk M, Zhang H, Li G, Hermanrud C, Neville D, et al. A truncated diphtheria toxin based recombinant porcine CTLA-4 fusion toxin. J Immunol Methods. 2013;391:103-11 pubmed publisher
Zuccotti P, Cartelli D, Stroppi M, Pandini V, Venturin M, Aliverti A, et al. Centaurin-?? interacts with ?-tubulin and stabilizes microtubules. PLoS ONE. 2012;7:e52867 pubmed publisher
LaRock C, Cookson B. The Yersinia virulence effector YopM binds caspase-1 to arrest inflammasome assembly and processing. Cell Host Microbe. 2012;12:799-805 pubmed publisher
Krahn M, Bückers J, Kastrup L, Wodarz A. Formation of a Bazooka-Stardust complex is essential for plasma membrane polarity in epithelia. J Cell Biol. 2010;190:751-60 pubmed publisher
Holzhausen L, Lewis A, Cheong K, Brockerhoff S. Differential role for synaptojanin 1 in rod and cone photoreceptors. J Comp Neurol. 2009;517:633-44 pubmed publisher
Marbouty M, Saguez C, Cassier Chauvat C, Chauvat F. Characterization of the FtsZ-interacting septal proteins SepF and Ftn6 in the spherical-celled cyanobacterium Synechocystis strain PCC 6803. J Bacteriol. 2009;191:6178-85 pubmed publisher
Osoata G, Yamamura S, Ito M, Vuppusetty C, Adcock I, Barnes P, et al. Nitration of distinct tyrosine residues causes inactivation of histone deacetylase 2. Biochem Biophys Res Commun. 2009;384:366-71 pubmed publisher
Alce T, Popik W. APOBEC3G is incorporated into virus-like particles by a direct interaction with HIV-1 Gag nucleocapsid protein. J Biol Chem. 2004;279:34083-6 pubmed
Ding Q, Fukami S, Meng X, Nishizaki Y, Zhang X, Sasaki H, et al. Mouse suppressor of fused is a negative regulator of sonic hedgehog signaling and alters the subcellular distribution of Gli1. Curr Biol. 1999;9:1119-22 pubmed
product information
Product Type :
Antibody
Product Name :
6x-His Tag Monoclonal Antibody (4A12E4)
Catalog # :
37-2900
Quantity :
100 ug
Price :
US 469.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Tag
Applications :
Immunocytochemistry: 5 ug/mL, Western Blot: 0.5 - 5 ug/mL
Species :
Tag
Clone :
4A12E4
Isotype :
IgG1, kappa
Storage :
-20 C
Description :
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Immunogen :
Synthetic peptide containing six His residues (6xHis).
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 5 ug/mL, Western Blot: 0.5 - 5 ug/mL
Aliases :
6His tag; 6X His; His Tag; Histidine Tag
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA