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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
EphA4 Monoclonal Antibody (4C8H5)
catalog :
37-1600
quantity :
100 ug
price :
US 471.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4C8H5
reactivity :
human, mouse, chicken
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation
more info or order :
Invitrogen product webpage
citations: 27
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:250; loading ...; fig 10a
Matsuura K, Kobayashi S, Konno K, Yamasaki M, Horiuchi T, Senda T, et al. SIPA1L1/SPAR1 Interacts with the Neurabin Family of Proteins and is Involved in GPCR Signaling. J Neurosci. 2022;42:2448-2473 pubmed publisher
  • western blot; human; loading ...; fig 7d
TerBush A, Hafkamp F, Lee H, Coscoy L. A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins α3β1, αVβ3, and αVβ5. J Virol. 2018;92: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3a
Rothe M, Kanwal N, Dietmann P, Seigfried F, Hempel A, Schütz D, et al. An Epha4/Sipa1l3/Wnt pathway regulates eye development and lens maturation. Development. 2017;144:321-333 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 4a
  • western blot; human; loading ...; fig 4a
Jurek A, Genander M, Kundu P, Catchpole T, He X, Strååt K, et al. Eph receptor interclass cooperation is required for the regulation of cell proliferation. Exp Cell Res. 2016;348:10-22 pubmed publisher
  • western blot; mouse; 1:200
Joly S, Jordi N, Schwab M, Pernet V. The Ephrin receptor EphA4 restricts axonal sprouting and enhances branching in the injured mouse optic nerve. Eur J Neurosci. 2014;40:3021-31 pubmed publisher
  • western blot; mouse; 1:200; fig 3
Kempf A, Montani L, Petrinovic M, Schroeter A, Weinmann O, Patrignani A, et al. Upregulation of axon guidance molecules in the adult central nervous system of Nogo-A knockout mice restricts neuronal growth and regeneration. Eur J Neurosci. 2013;38:3567-79 pubmed publisher
  • western blot; mouse; 1:1000; fig s2
Lemmens R, Jaspers T, Robberecht W, Thijs V. Modifying expression of EphA4 and its downstream targets improves functional recovery after stroke. Hum Mol Genet. 2013;22:2214-20 pubmed publisher
  • immunohistochemistry; mouse
Semerdjieva S, Abdul Razak H, Salim S, Yáñez Muñoz R, Chen P, Tarabykin V, et al. Activation of EphA receptors mediates the recruitment of the adaptor protein Slap, contributing to the downregulation of N-methyl-D-aspartate receptors. Mol Cell Biol. 2013;33:1442-55 pubmed publisher
  • western blot; mouse; 1:1000; fig 4
Van Hoecke A, Schoonaert L, Lemmens R, Timmers M, Staats K, Laird A, et al. EPHA4 is a disease modifier of amyotrophic lateral sclerosis in animal models and in humans. Nat Med. 2012;18:1418-22 pubmed
  • immunoprecipitation; mouse; fig 5
Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisen J, et al. Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction. PLoS ONE. 2008;3:e3322 pubmed publisher
  • western blot; mouse; fig 1
Beg A, Sommer J, Martin J, Scheiffele P. alpha2-Chimaerin is an essential EphA4 effector in the assembly of neuronal locomotor circuits. Neuron. 2007;55:768-78 pubmed
Zhang S, Zhao J, Sha W, Zhang X, Mai J, Bartlett P, et al. Inhibition of EphA4 reduces vasogenic edema after experimental stroke in mice by protecting the blood-brain barrier integrity. J Cereb Blood Flow Metab. 2024;44:419-433 pubmed publisher
Liang X, Fa W, Wang N, Peng Y, Liu C, Zhu M, et al. Exosomal miR-532-5p induced by long-term exercise rescues blood-brain barrier function in 5XFAD mice via downregulation of EPHA4. Aging Cell. 2023;22:e13748 pubmed publisher
Dennys C, Baggio C, Rodrigo R, Roussel F, Kulinich A, Heintzman S, et al. EphA4 targeting agents protect motor neurons from cell death induced by amyotrophic lateral sclerosis -astrocytes. iScience. 2022;25:104877 pubmed publisher
Wolff D, Deng Z, Bianchi Smiraglia A, Foley C, Han Z, Wang X, et al. Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4. Cell Chem Biol. 2022;29:970-984.e6 pubmed publisher
Baggio C, Kulinich A, Dennys C, Rodrigo R, Meyer K, ETHELL I, et al. NMR-Guided Design of Potent and Selective EphA4 Agonistic Ligands. J Med Chem. 2021;64:11229-11246 pubmed publisher
Poppe L, Rue L, Timmers M, Lenaerts A, Storm A, Callaerts Vegh Z, et al. EphA4 loss improves social memory performance and alters dendritic spine morphology without changes in amyloid pathology in a mouse model of Alzheimer's disease. Alzheimers Res Ther. 2019;11:102 pubmed publisher
de Boer A, Storm A, Gómez Soler M, Smolders S, Rue L, Poppe L, et al. Environmental enrichment during the chronic phase after experimental stroke promotes functional recovery without synergistic effects of EphA4 targeted therapy. Hum Mol Genet. 2020;29:605-617 pubmed publisher
Poppe L, Smolders S, Rue L, Timmers M, Lenaerts A, Storm A, et al. Lowering EphA4 Does Not Ameliorate Disease in a Mouse Model for Severe Spinal Muscular Atrophy. Front Neurosci. 2019;13:1233 pubmed publisher
Kowalski E, Chen J, Hazy A, Fritsch L, Gudenschwager Basso E, Chen M, et al. Peripheral loss of EphA4 ameliorates TBI-induced neuroinflammation and tissue damage. J Neuroinflammation. 2019;16:210 pubmed publisher
Rue L, Timmers M, Lenaerts A, Smolders S, Poppe L, de Boer A, et al. Reducing EphA4 before disease onset does not affect survival in a mouse model of Amyotrophic Lateral Sclerosis. Sci Rep. 2019;9:14112 pubmed publisher
García Diaz B, Bachelin C, Coulpier F, Gerschenfeld G, Deboux C, Zujovic V, et al. Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling. Acta Neuropathol. 2019;: pubmed publisher
Chen J, Sathiyamoorthy K, Zhang X, Schaller S, Perez White B, Jardetzky T, et al. Ephrin receptor A2 is a functional entry receptor for Epstein-Barr virus. Nat Microbiol. 2018;3:172-180 pubmed publisher
Wu B, De S, Kulinich A, Salem A, Koeppen J, Wang R, et al. Potent and Selective EphA4 Agonists for the Treatment of ALS. Cell Chem Biol. 2017;24:293-305 pubmed publisher
Roher A, Maarouf C, Kokjohn T, Whiteside C, Kalback W, Serrano G, et al. Neuropathological and biochemical assessments of an Alzheimer's disease patient treated with the γ-secretase inhibitor semagacestat. Am J Neurodegener Dis. 2014;3:115-33 pubmed
Noberini R, Rubio de la Torre E, Pasquale E. Profiling Eph receptor expression in cells and tissues: a targeted mass spectrometry approach. Cell Adh Migr. 2012;6:102-12 pubmed publisher
Richter M, Murai K, Bourgin C, Pak D, Pasquale E. The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases. J Neurosci. 2007;27:14205-15 pubmed
product information
Product Type :
Antibody
Product Name :
EphA4 Monoclonal Antibody (4C8H5)
Catalog # :
37-1600
Quantity :
100 ug
Price :
US 471.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Chicken, Human, Mouse
Applications :
ELISA: Assay-dependent, Immunohistochemistry: 3-10 ug/mL, Immunoprecipitation: 5 ug, Western Blot: 1-3 ug/mL
Species :
Chicken, Human, Mouse
Clone :
4C8H5
Isotype :
IgG1, kappa
Storage :
-20 C
Description :
EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA4 encodes a protein that binds ephrin-A ligands.
Immunogen :
A synthetic peptide derived from the C-terminus human EphA4 receptor protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunohistochemistry: 3-10 ug/mL, Immunoprecipitation: 5 ug, Western Blot: 1-3 ug/mL
Aliases :
2900005C20Rik; AI385584; Cek8; Cek8 protein; EK8; epa4; EPH receptor A4; Epha4; EPH-like kinase 8; Ephrin type-A receptor 4; Hek8; rb; receptor protein-tyrosine kinase HEK8; RGD1560587; Sek; Sek1; Tyro1; TYRO1 protein tyrosine kinase; tyrosine-protein kinase receptor MPK-3; Tyrosine-protein kinase receptor SEK; tyrosine-protein kinase receptor SEK-1; Tyrosine-protein kinase TYRO1
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA