UBC13 Monoclonal Antibody (4E 11) | Invitrogen 37-1100 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

UBC13 Monoclonal Antibody (4E 11)

catalog :

37-1100

quantity :

100 µg

price :

US 450

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4E 11

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 37

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig 5 immunoprecipitation; human; fig 3 western blot; human	Shembade N, Harhaj N, Yamamoto M, Akira S, Harhaj E. The human T-cell leukemia virus type 1 Tax oncoprotein requires the ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation. J Virol. 2007;81:13735-42 pubmed
western blot; mouse; loading ...; fig s4a	Zhang H, Li H, Hillmer E, Zhao Y, Chrisikos T, Hu H, et al. Genetic rescue of lineage-balanced blood cell production reveals a crucial role for STAT3 antiinflammatory activity in hematopoiesis. Proc Natl Acad Sci U S A. 2018;115:E2311-E2319 pubmed publisher
western blot; human; loading ...; fig 3c	Paul A, Wang B. RNF8- and Ube2S-Dependent Ubiquitin Lysine 11-Linkage Modification in Response to DNA Damage. Mol Cell. 2017;66:458-472.e5 pubmed publisher
western blot; human; loading ...; fig 3c	Zhang J, MacArtney T, Peggie M, Cohen P. Interleukin-1 and TRAF6-dependent activation of TAK1 in the absence of TAB2 and TAB3. Biochem J. 2017;474:2235-2248 pubmed publisher
western blot; mouse; loading ...; fig 1c	Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, et al. HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains. PLoS Pathog. 2017;13:e1006162 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3c	Weber E, Rothenaigner I, Brandner S, Hadian K, Schorpp K. A High-Throughput Screening Strategy for Development of RNF8-Ubc13 Protein-Protein Interaction Inhibitors. SLAS Discov. 2017;22:316-323 pubmed publisher
western blot; mouse; 1:1000; fig 1g	Zhang H, Hu H, Greeley N, Jin J, Matthews A, Ohashi E, et al. STAT3 restrains RANK- and TLR4-mediated signalling by suppressing expression of the E2 ubiquitin-conjugating enzyme Ubc13. Nat Commun. 2014;5:5798 pubmed publisher
western blot; mouse; 1:1,000	Shiba Fukushima K, Inoshita T, Hattori N, Imai Y. Lysine 63-linked polyubiquitination is dispensable for Parkin-mediated mitophagy. J Biol Chem. 2014;289:33131-6 pubmed publisher
western blot; human; 1:1000; fig s1	Fiesel F, Moussaud Lamodière E, Ando M, Springer W. A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently. J Cell Sci. 2014;127:3488-504 pubmed publisher
western blot; human	Cheng J, Fan Y, Xu X, Dou J, Tang Y, Zhong X, et al. A small-molecule inhibitor of UBE2N induces neuroblastoma cell death via activation of p53 and JNK pathways. Cell Death Dis. 2014;5:e1079 pubmed publisher
western blot; mouse	Fan Y, Mao R, Yu Y, Liu S, Shi Z, Cheng J, et al. USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase. J Exp Med. 2014;211:313-28 pubmed publisher
western blot; human; 1:1000; fig 5	Wiener R, Dibello A, Lombardi P, Guzzo C, Zhang X, Matunis M, et al. E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nat Struct Mol Biol. 2013;20:1033-9 pubmed publisher
western blot; human; fig 8	Pulvino M, Liang Y, Oleksyn D, DeRan M, Van Pelt E, Shapiro J, et al. Inhibition of proliferation and survival of diffuse large B-cell lymphoma cells by a small-molecule inhibitor of the ubiquitin-conjugating enzyme Ubc13-Uev1A. Blood. 2012;120:1668-77 pubmed publisher
western blot; human; 1:1000; fig s4	Ciccia A, Nimonkar A, Hu Y, Hajdu I, Achar Y, Izhar L, et al. Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress. Mol Cell. 2012;47:396-409 pubmed publisher
immunoprecipitation; human western blot; human	Stewart G, Panier S, Townsend K, Al Hakim A, Kolas N, Miller E, et al. The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage. Cell. 2009;136:420-34 pubmed publisher
western blot; human	Sakasai R, Tibbetts R. RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage. J Biol Chem. 2008;283:13549-55 pubmed publisher
western blot; mouse; fig 1	Yamamoto M, Sato S, Saitoh T, Sakurai H, Uematsu S, Kawai T, et al. Cutting Edge: Pivotal function of Ubc13 in thymocyte TCR signaling. J Immunol. 2006;177:7520-4 pubmed
	Lenoir J, Parisien J, Horvath C. Immune regulator LGP2 targets Ubc13/UBE2N to mediate widespread interference with K63 polyubiquitination and NF-κB activation. Cell Rep. 2021;37:110175 pubmed publisher
	Wu X, Wang B. Abraxas suppresses DNA end resection and limits break-induced replication by controlling SLX4/MUS81 chromatin loading in response to TOP1 inhibitor-induced DNA damage. Nat Commun. 2021;12:4373 pubmed publisher
	Wambecke A, Ahmad M, Morice P, Lambert B, Weiswald L, Vernon M, et al. The lncRNA 'UCA1' modulates the response to chemotherapy of ovarian cancer through direct binding to miR-27a-5p and control of UBE2N levels. Mol Oncol. 2021;15:3659-3678 pubmed publisher
	Ni J, Guan C, Liu H, Huang X, Yue J, Xiang H, et al. Ubc13 Promotes K63-Linked Polyubiquitination of NLRP3 to Activate Inflammasome. J Immunol. 2021;206:2376-2385 pubmed publisher
	Puvar K, Iyer S, Fu J, Kenny S, Negrón Terón K, Luo Z, et al. Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination. Nat Commun. 2020;11:2365 pubmed publisher
	Gan N, Guan H, Huang Y, Yu T, Fu J, Nakayasu E, et al. Legionella pneumophila regulates the activity of UBE2N by deamidase-mediated deubiquitination. EMBO J. 2020;39:e102806 pubmed publisher
	Romero Barrios N, Monachello D, Dolde U, Wong A, San Clemente H, Cayrel A, et al. Advanced Cataloging of Lysine-63 Polyubiquitin Networks by Genomic, Interactome, and Sensor-Based Proteomic Analyses. Plant Cell. 2020;32:123-138 pubmed publisher
	Gan N, Nakayasu E, Hollenbeck P, Luo Z. Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N. Nat Microbiol. 2019;4:134-143 pubmed publisher
	Pasupala N, Morrow M, Que L, Malynn B, Ma A, Wolberger C. OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating enzyme UBE2E1 by preventing its autoubiquitination. J Biol Chem. 2018;293:18285-18295 pubmed publisher
	Brenke J, Popowicz G, Schorpp K, Rothenaigner I, Roesner M, Meininger I, et al. Targeting TRAF6 E3 ligase activity with a small-molecule inhibitor combats autoimmunity. J Biol Chem. 2018;293:13191-13203 pubmed publisher
	Haahr P, Borgermann N, Guo X, Typas D, Achuthankutty D, Hoffmann S, et al. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability. Mol Cell. 2018;70:165-174.e6 pubmed publisher
	Waraky A, Lin Y, Warsito D, Haglund F, Aleem E, Larsson O. Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage. J Biol Chem. 2017;292:18227-18239 pubmed publisher
	Vujanovic M, Krietsch J, Raso M, Terraneo N, Zellweger R, Schmid J, et al. Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity. Mol Cell. 2017;67:882-890.e5 pubmed publisher
	Hodge C, Edwards R, Markin C, McDonald D, Pulvino M, Huen M, et al. Covalent Inhibition of Ubc13 Affects Ubiquitin Signaling and Reveals Active Site Elements Important for Targeting. ACS Chem Biol. 2015;10:1718-28 pubmed publisher
	Pujari R, Hunte R, Thomas R, van der Weyden L, Rauch D, Ratner L, et al. Human T-cell leukemia virus type 1 (HTLV-1) tax requires CADM1/TSLC1 for inactivation of the NF-κB inhibitor A20 and constitutive NF-κB signaling. PLoS Pathog. 2015;11:e1004721 pubmed publisher
	Cotta Ramusino C, McDonald E, Hurov K, Sowa M, Harper J, Elledge S. A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling. Science. 2011;332:1313-7 pubmed publisher
	Wu X, Yamamoto M, Akira S, Sun S. Regulation of hematopoiesis by the K63-specific ubiquitin-conjugating enzyme Ubc13. Proc Natl Acad Sci U S A. 2009;106:20836-41 pubmed publisher
	Huen M, Huang J, Yuan J, Yamamoto M, Akira S, Ashley C, et al. Noncanonical E2 variant-independent function of UBC13 in promoting checkpoint protein assembly. Mol Cell Biol. 2008;28:6104-12 pubmed publisher
	Ea C, Sun L, Inoue J, Chen Z. TIFA activates IkappaB kinase (IKK) by promoting oligomerization and ubiquitination of TRAF6. Proc Natl Acad Sci U S A. 2004;101:15318-23 pubmed
	Habelhah H, Takahashi S, Cho S, Kadoya T, Watanabe T, RONAI Z. Ubiquitination and translocation of TRAF2 is required for activation of JNK but not of p38 or NF-kappaB. EMBO J. 2004;23:322-32 pubmed

product information

Product Type :

Antibody

Product Name :

UBC13 Monoclonal Antibody (4E 11)

Catalog # :

37-1100

Quantity :

100 µg

Price :

US 450

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: Assay-dependent, Immunocytochemistry: Assay-dependent, Western Blot: Assay-dependent

Species :

Human, Mouse, Rat

Clone :

4E 11

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. (Uniprot)

Immunogen :

Recombinant human Ubc13

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: Assay-dependent, Western Blot: Assay-dependent

Aliases :

1500026J17Rik; AL022654; BB101821; bendless protein; bendless-like ubiquitin conjugating enzyme; bendless-like ubiquitin-conjugating enzyme; Blu; E2 ubiquitin-conjugating enzyme N; epididymis secretory protein Li 71; HEL-S-71; MGC131857; MGC8489; UBC13; UbcH13; UbcH-ben; UBCHBEN; UBC13; UBE2N; Ubiquitin carrier protein N; ubiquitin conjugating enzyme E2 N; ubiquitin conjugating enzyme E2N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-protein ligase N; yeast UBC13 homolog

more info or order :

Invitrogen product webpage