TSC1 Monoclonal Antibody (5C8A12) | Invitrogen 37-0400 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TSC1 Monoclonal Antibody (5C8A12)

catalog :

37-0400

quantity :

100 µg

price :

US 450

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5C8A12

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig 1	Valianou M, Cox A, Pichette B, Hartley S, Paladhi U, Astrinidis A. Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy. Cell Cycle. 2015;14:399-407 pubmed publisher
western blot; human; fig 5	Liang N, Zhang C, Dill P, Panasyuk G, Pion D, Koka V, et al. Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex. J Exp Med. 2014;211:2249-63 pubmed publisher
western blot; human; fig 1	Yen C, Lin Y, Yen C, Tsai H, Tsai T, Chang K, et al. Hepatitis B virus X protein upregulates mTOR signaling through IKKÎ² to increase cell proliferation and VEGF production in hepatocellular carcinoma. PLoS ONE. 2012;7:e41931 pubmed publisher
western blot; human; fig 3	Kume K, Iizumi Y, Shimada M, Ito Y, Kishi T, Yamaguchi Y, et al. Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway. Genes Cells. 2010;15:339-49 pubmed publisher
western blot; human; fig 4d	Kuo H, Lee D, Chen C, Liu M, Chou C, Lee H, et al. ARD1 stabilization of TSC2 suppresses tumorigenesis through the mTOR signaling pathway. Sci Signal. 2010;3:ra9 pubmed publisher
immunocytochemistry; human; 1:100; fig 1 western blot; human; fig 2	Hartman T, Liu D, Zilfou J, Robb V, Morrison T, Watnick T, et al. The tuberous sclerosis proteins regulate formation of the primary cilium via a rapamycin-insensitive and polycystin 1-independent pathway. Hum Mol Genet. 2009;18:151-63 pubmed publisher
immunohistochemistry - paraffin section; human; 1:70; tbl 1	Boer K, Troost D, Jansen F, Nellist M, van den Ouweland A, Geurts J, et al. Clinicopathological and immunohistochemical findings in an autopsy case of tuberous sclerosis complex. Neuropathology. 2008;28:577-90 pubmed publisher
western blot; mouse; fig 4a	Lee D, Kuo H, Chen C, Hsu J, Chou C, Wei Y, et al. IKK beta suppression of TSC1 links inflammation and tumor angiogenesis via the mTOR pathway. Cell. 2007;130:440-55 pubmed
	Danielson L, Guijarro M, Menendez S, Higgins B, Sun Q, Mittal K, et al. MiR-130b modulates the invasive, migratory, and metastatic behavior of leiomyosarcoma. PLoS ONE. 2023;18:e0278844 pubmed publisher
	Prentzell M, Rehbein U, Cadena Sandoval M, De Meulemeester A, Baumeister R, Brohée L, et al. G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling. Cell. 2021;184:655-674.e27 pubmed publisher
	Ko C, Zhang L, Jie Z, Zhu L, Zhou X, Xie X, et al. The E3 ubiquitin ligase Peli1 regulates the metabolic actions of mTORC1 to suppress antitumor T cell responses. EMBO J. 2021;40:e104532 pubmed publisher
	Streleckiene G, Inciuraite R, Juzenas S, Salteniene V, Steponaitiene R, Gyvyte U, et al. miR-20b and miR-451a Are Involved in Gastric Carcinogenesis through the PI3K/AKT/mTOR Signaling Pathway: Data from Gastric Cancer Patients, Cell Lines and Ins-Gas Mouse Model. Int J Mol Sci. 2020;21: pubmed publisher
	Wang C, Uemura M, Tomiyama E, Matsushita M, Koh Y, Nakano K, et al. MicroRNA-92b-3p is a prognostic oncomiR that targets TSC1 in clear cell renal cell carcinoma. Cancer Sci. 2020;111:1146-1155 pubmed publisher
	Cloutier P, Poitras C, Durand M, Hekmat O, Fiola Masson É, Bouchard A, et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun. 2017;8:15615 pubmed publisher
	Menon S, Dibble C, Talbott G, Hoxhaj G, Valvezan A, Takahashi H, et al. Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome. Cell. 2014;156:771-85 pubmed publisher
	Dibble C, Elis W, Menon S, Qin W, Klekota J, Asara J, et al. TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1. Mol Cell. 2012;47:535-46 pubmed publisher

product information

Product Type :

Antibody

Product Name :

TSC1 Monoclonal Antibody (5C8A12)

Catalog # :

37-0400

Quantity :

100 µg

Price :

US 450

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Human

Clone :

5C8A12

Isotype :

IgG1

Storage :

-20°C

Description :

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

Immunogen :

Recombinant human Hamartin protein

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Aliases :

hamartin; HGNC:12362; Kiaa0243; LAM; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; TSC; Tsc1; tuberous sclerosis 1; tuberous sclerosis 1 protein; Tuberous sclerosis 1 protein homolog; tumor suppressor

more info or order :

Invitrogen product webpage