GRP94 Monoclonal Antibody (9G10)
Invitrogen
catalog: MA3-016
rat monoclonal (9G10)
reactivity: Chinese hamster, human, mouse, rat, chicken, cow
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 19

Immunofluorescent analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) (Product# MA3-016 ) shows staining in C6 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 (Product# MA3-016 ) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse breast tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Glucose Regulated Protein 94 (MA3-016) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100µL
price: US 390
to the supplier
KDEL Polyclonal Antibody
Invitrogen
catalog: PA1-013
rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 8
quantity: 100µg
price: US 390
to the supplier
GRP94 Monoclonal Antibody (9G10.F8.2)
Invitrogen
catalog: MA5-14592
rat monoclonal (9G10.F8.2)
reactivity: Domestic guinea pig, Chinese hamster, human, mouse, rat, dog, chicken, cow, pig, sheep, common platanna, rabbit, equine
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 4
quantity: 500µL
price: US 325
to the supplier
GRP94 Polyclonal Antibody
Invitrogen
catalog: 36-2600
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 2
quantity: 100µg
price: US 345
to the supplier
KDEL Polyclonal Antibody, Alexa Fluor 488
Invitrogen
catalog: PA1-013-A488
rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat
conjugate: AF488
application: immunocytochemistry
citations: 3
quantity: 50µl
price: US 390
to the supplier
KDEL Polyclonal Antibody, Alexa Fluor 555
Invitrogen
catalog: PA1-013-A555
rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat
conjugate: Alexa Fluor 555
application: immunocytochemistry
citations: 3
quantity: 50µl
price: US 305
to the supplier
KDEL Polyclonal Antibody, Alexa Fluor 647
Invitrogen
catalog: PA1-013-A647
rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat
conjugate: AF647
application: immunocytochemistry
citations: 3
quantity: 50µl
price: US 305
to the supplier
GRP94 Polyclonal Antibody
Invitrogen
catalog: PA5-17280
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

quantity: 100µL
price: US 345
to the supplier
GRP94 Polyclonal Antibody
Invitrogen
catalog: PA5-18534
goat polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Immunohistochemical staining of paraffin embedded of Human Liver using PA5-18534 at a concentration of 3.8 ug/ml. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.

Western Blot staining of NIH/3T3 cell lysate using PA5-18534 at a concentration of 0.03 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
quantity: 100µg
price: US 345
to the supplier