Phospho-EGFR (Tyr1086) Polyclonal Antibody | Invitrogen 36-9700 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-EGFR (Tyr1086) Polyclonal Antibody

catalog :

36-9700

quantity :

50 µg

price :

US 425.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:250; loading ...; fig s4i	Gut G, Herrmann M, Pelkmans L. Multiplexed protein maps link subcellular organization to cellular states. Science. 2018;361: pubmed publisher
western blot; human; 1:1000; fig s2a	Mai W, Gosa L, Daniëls V, Ta L, Tsang J, Higgins B, et al. Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nat Med. 2017;23:1342-1351 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 4a western blot; human; loading ...; fig 4d	Zhang Y, Yang J, Ding M, Li L, Lu Z, Zhang Q, et al. Tumor-penetration and antitumor efficacy of cetuximab are enhanced by co-administered iRGD in a murine model of human NSCLC. Oncol Lett. 2016;12:3241-3249 pubmed
	Fassunke J, Müller F, Keul M, Michels S, Dammert M, Schmitt A, et al. Overcoming EGFRG724S-mediated osimertinib resistance through unique binding characteristics of second-generation EGFR inhibitors. Nat Commun. 2018;9:4655 pubmed publisher
	Yang B, Qian F, Li W, Li Y, Han Y. Effects of general anesthesia with or without epidural block on tumor metastasis and mechanisms. Oncol Lett. 2018;15:4662-4668 pubmed publisher
	Wong C, Ma B, Hui C, Tao Q, Chan A. Preclinical evaluation of afatinib (BIBW2992) in esophageal squamous cell carcinoma (ESCC). Am J Cancer Res. 2015;5:3588-99 pubmed

product information

Product Type :

Antibody

Product Name :

Phospho-EGFR (Tyr1086) Polyclonal Antibody

Catalog # :

36-9700

Quantity :

50 µg

Price :

US 425.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry: Assay-dependent, Immunoprecipitation: 0.1-0.5 µg/mL, Western Blot: 0.5 µg/mL

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20°C

Description :

EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Immunogen :

Synthetic peptide derived from the region surrounding the phosphorylated Tyr1086 residue of the human, mouse, rat, and pig epidermal growth factor receptor (EGFr) protein.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry: Assay-dependent, Immunoprecipitation: 0.1-0.5 µg/mL, Western Blot: 0.5 µg/mL

Aliases :

2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2

more info or order :

Invitrogen product webpage