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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Claudin 4 Polyclonal Antibody (ZMD.306)
catalog :
36-4800
quantity :
100 µg
price :
US 450
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
ZMD.306
reactivity :
human, mouse, rat, domestic horse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:100; fig 3a, 5a
Tabariès S, Annis M, Lazaris A, Petrillo S, Huxham J, Abdellatif A, et al. Claudin-2 promotes colorectal cancer liver metastasis and is a biomarker of the replacement type growth pattern. Commun Biol. 2021;4:657 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6d
Xing T, Benderman L, Sabu S, Parker J, Yang J, Lu Q, et al. Tight Junction Protein Claudin-7 Is Essential for Intestinal Epithelial Stem Cell Self-Renewal and Differentiation. Cell Mol Gastroenterol Hepatol. 2020;9:641-659 pubmed publisher
  • immunocytochemistry; mouse; fig 4d
Miyoshi H, VanDussen K, Malvin N, Ryu S, Wang Y, Sonnek N, et al. Prostaglandin E2 promotes intestinal repair through an adaptive cellular response of the epithelium. EMBO J. 2017;36:5-24 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig 9
  • western blot; mouse; 1:1000; fig 5
Dianati E, Poiraud J, Weber Ouellette A, Plante I. Connexins, E-cadherin, Claudin-7 and ?-catenin transiently form junctional nexuses during the post-natal mammary gland development. Dev Biol. 2016;416:52-68 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 1
Kobayashi K, Tsugami Y, Matsunaga K, Oyama S, Kuki C, Kumura H. Prolactin and glucocorticoid signaling induces lactation-specific tight junctions concurrent with ?-casein expression in mammary epithelial cells. Biochim Biophys Acta. 2016;1863:2006-16 pubmed publisher
  • immunohistochemistry - paraffin section; domestic horse; fig 4
  • western blot; domestic horse; 1:1000; fig 1
Lee B, Kang H, Lee D, Ahn C, Jeung E. Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues. J Vet Sci. 2016;17:445-451 pubmed publisher
Wittner L, Wagener L, Wiese J, Stolzer I, Krug S, Naschberger E, et al. Proteolytic Activity of the Paracaspase MALT1 Is Involved in Epithelial Restitution and Mucosal Healing. Int J Mol Sci. 2023;24: pubmed publisher
Bekusova V, Zudova T, Fatyykhov I, Fedorova A, Amasheh S, Markov A. Selective Role of TNFα and IL10 in Regulation of Barrier Properties of the Colon in DMH-Induced Tumor and Healthy Rats. Int J Mol Sci. 2022;23: pubmed publisher
Peart N, Hwang J, Quesnel Valli xe8 res M, Sears M, Yang Y, Stoilov P, et al. The global Protein-RNA interaction map of ESRP1 defines a post-transcriptional program that is essential for epithelial cell function. iScience. 2022;25:105205 pubmed publisher
Delgado Diaz D, Jesaveluk B, Hayward J, Tyssen D, Alisoltani A, Potgieter M, et al. Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression. Microbiome. 2022;10:141 pubmed publisher
Koumangoye R, Penny P, Delpire E. Loss of NKCC1 function increases epithelial tight junction permeability by upregulating claudin-2 expression. Am J Physiol Cell Physiol. 2022;323:C1251-C1263 pubmed publisher
Yu H, Lin Y, Zhong Y, Guo X, Lin Y, Yang S, et al. Impaired AT2 to AT1 cell transition in PM2.5-induced mouse model of chronic obstructive pulmonary disease. Respir Res. 2022;23:70 pubmed publisher
Jeon H, Choi J, Kraaier L, Kim Y, Eisenbarth D, Yi K, et al. Airway secretory cell fate conversion via YAP-mTORC1-dependent essential amino acid metabolism. EMBO J. 2022;41:e109365 pubmed publisher
Apostolou A, Panchakshari R, Banerjee A, Manatakis D, Paraskevopoulou M, Luc R, et al. A Novel Microphysiological Colon Platform to Decipher Mechanisms Driving Human Intestinal Permeability. Cell Mol Gastroenterol Hepatol. 2021;12:1719-1741 pubmed publisher
Broussard J, Koetsier J, Hegazy M, Green K. Desmosomes polarize and integrate chemical and mechanical signaling to govern epidermal tissue form and function. Curr Biol. 2021;31:3275-3291.e5 pubmed publisher
Fan L, Qi Y, Qu S, Chen X, Li A, Hendi M, et al. B. adolescentis ameliorates chronic colitis by regulating Treg/Th2 response and gut microbiota remodeling. Gut Microbes. 2021;13:1-17 pubmed publisher
Jia H, Zhang Y, Si X, Jin Y, Jiang D, Dai Z, et al. Quercetin Alleviates Oxidative Damage by Activating Nuclear Factor Erythroid 2-Related Factor 2 Signaling in Porcine Enterocytes. Nutrients. 2021;13: pubmed publisher
Tinh N, Sitolo G, Yamamoto Y, Suzuki T. Citrus limon Peel Powder Reduces Intestinal Barrier Defects and Inflammation in a Colitic Murine Experimental Model. Foods. 2021;10: pubmed publisher
Cray P, Sheahan B, Cortes J, Dekaney C. Doxorubicin increases permeability of murine small intestinal epithelium and cultured T84 monolayers. Sci Rep. 2020;10:21486 pubmed publisher
Uc P, Miranda J, Raya Sandino A, Alarcón L, Roldán M, Ocadiz Delgado R, et al. E7 oncoprotein from human papillomavirus 16 alters claudins expression and the sealing of epithelial tight junctions. Int J Oncol. 2020;57:905-924 pubmed publisher
Choi J, Park J, Tsagkogeorga G, Yanagita M, Koo B, Han N, et al. Inflammatory Signals Induce AT2 Cell-Derived Damage-Associated Transient Progenitors that Mediate Alveolar Regeneration. Cell Stem Cell. 2020;27:366-382.e7 pubmed publisher
Marjanovic N, Hofree M, Chan J, Canner D, Wu K, Trakala M, et al. Emergence of a High-Plasticity Cell State during Lung Cancer Evolution. Cancer Cell. 2020;38:229-246.e13 pubmed publisher
Kobayashi Y, Tata A, Konkimalla A, Katsura H, Lee R, Ou J, et al. Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. Nat Cell Biol. 2020;22:934-946 pubmed publisher
Li J, Gordon J, Chen E, Xiao S, Wu L, Zuniga Pflucker J, et al. NOTCH1 signaling establishes the medullary thymic epithelial cell progenitor pool during mouse fetal development. Development. 2020;147: pubmed publisher
Quirós M, Feier D, Birkl D, Agarwal R, Zhou D, Garcia A, et al. Resolvin E1 is a pro-repair molecule that promotes intestinal epithelial wound healing. Proc Natl Acad Sci U S A. 2020;117:9477-9482 pubmed publisher
Kobayashi K, Tsugami Y, Suzuki N, Suzuki T, Nishimura T. Nicotine directly affects milk production in lactating mammary epithelial cells concurrently with inactivation of STAT5 and glucocorticoid receptor in vitro. Toxicol In Vitro. 2020;63:104741 pubmed publisher
Mehdizadeh Gohari I, Li J, Navarro M, Uzal F, McClane B. Effects of Claudin-1 on the Action of Clostridium perfringens Enterotoxin in Caco-2 Cells. Toxins (Basel). 2019;11: pubmed publisher
Gamero Estévez E, Andonian S, Jean Claude B, Gupta I, Ryan A. Temporal Effects of Quercetin on Tight Junction Barrier Properties and Claudin Expression and Localization in MDCK II Cells. Int J Mol Sci. 2019;20: pubmed publisher
Miranda J, Martín Tapia D, Valdespino Vazquez Y, Alarcón L, Espejel Nuñez A, Guzmán Huerta M, et al. Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability. Cells. 2019;8: pubmed publisher
Gunasekaran A, Eckert J, Burge K, Zheng W, Yu Z, Kessler S, et al. Hyaluronan 35 kDa enhances epithelial barrier function and protects against the development of murine necrotizing enterocolitis. Pediatr Res. 2020;87:1177-1184 pubmed publisher
Wagner T, Beer L, Gschwandtner M, Eckhart L, Kalinina P, Laggner M, et al. The differentiation-associated keratinocyte protein cornifelin contributes to cell-cell adhesion of epidermal and mucosal keratinocytes. J Invest Dermatol. 2019;: pubmed publisher
Ouyang X, Dong C, Ubogu E. In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves. J Peripher Nerv Syst. 2019;24:195-206 pubmed publisher
Becker J, Ott D, Diener M. Impact of Sensitization and Inflammation on the Interaction of Mast Cells With the Intestinal Epithelium in Rats. Front Physiol. 2019;10:329 pubmed publisher
Jang Y, Kim W, Han D, Lee K, Ko G. Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota. Gut Microbes. 2019;10:696-711 pubmed publisher
Suzuki K, Sentani K, Tanaka H, Yano T, Suzuki K, Oshima M, et al. Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection. Cell Mol Gastroenterol Hepatol. 2019;8:119-142 pubmed publisher
Lashhab R, Rumley A, Arutyunov D, Rizvi M, You C, Dimke H, et al. The kidney anion exchanger 1 affects tight junction properties via claudin-4. Sci Rep. 2019;9:3099 pubmed publisher
Logullo A, Pasini F, Nonogaki S, Rocha R, Soares F, Brentani M. Immunoexpression of claudins 4 and 7 among invasive breast carcinoma subtypes: A large diagnostic study using tissue microarray. Mol Clin Oncol. 2018;9:377-388 pubmed publisher
Palladino S, Helton E, Jain P, Dong C, Crowley M, Crossman D, et al. The Human Blood-Nerve Barrier Transcriptome. Sci Rep. 2017;7:17477 pubmed publisher
Shimada H, Satohisa S, Kohno T, Konno T, Takano K, Takahashi S, et al. Downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion via claudin-1-mediated matrix metalloproteinases in human endometrial cancer. Oncol Lett. 2017;14:6776-6782 pubmed publisher
Ahmad R, Rah B, Bastola D, Dhawan P, Singh A. Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching. Sci Rep. 2017;7:5125 pubmed publisher
Gao X, Bali A, Randell S, Hogan B. GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells. J Cell Biol. 2015;211:669-82 pubmed publisher
Gruber R, Börnchen C, Rose K, Daubmann A, Volksdorf T, Wladykowski E, et al. Diverse regulation of claudin-1 and claudin-4 in atopic dermatitis. Am J Pathol. 2015;185:2777-89 pubmed publisher
Lu Z, Kim D, Fan J, Lu Q, Verbanac K, Ding L, et al. A non-tight junction function of claudin-7-Interaction with integrin signaling in suppressing lung cancer cell proliferation and detachment. Mol Cancer. 2015;14:120 pubmed publisher
Staat C, Coisne C, Dabrowski S, Stamatovic S, Andjelkovic A, Wolburg H, et al. Mode of action of claudin peptidomimetics in the transient opening of cellular tight junction barriers. Biomaterials. 2015;54:9-20 pubmed publisher
Overgaard C, Schlingmann B, Dorsainvil White S, Ward C, Fan X, Swarnakar S, et al. The relative balance of GM-CSF and TGF-β1 regulates lung epithelial barrier function. Am J Physiol Lung Cell Mol Physiol. 2015;308:L1212-23 pubmed publisher
Ward C, Schlingmann B, Stecenko A, Guidot D, Koval M. NF-κB inhibitors impair lung epithelial tight junctions in the absence of inflammation. Tissue Barriers. 2015;3:e982424 pubmed publisher
Fredriksson K, Van Itallie C, Aponte A, Gucek M, Tietgens A, Anderson J. Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks. PLoS ONE. 2015;10:e0117074 pubmed publisher
García Hernández V, Flores Maldonado C, Rincon Heredia R, Verdejo Torres O, Bonilla Delgado J, Meneses Morales I, et al. EGF regulates claudin-2 and -4 expression through Src and STAT3 in MDCK cells. J Cell Physiol. 2015;230:105-15 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Claudin 4 Polyclonal Antibody (ZMD.306)
Catalog # :
36-4800
Quantity :
100 µg
Price :
US 450
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Mouse, Rat
Applications :
ELISA: 0.1-1 µg/mL, Immunocytochemistry: Assay-dependent, Immunoprecipitation: 1-3 µg/mL, Western Blot: 1-3 µg/mL
Species :
Mouse, Rat
Clone :
ZMD.306
Isotype :
IgG
Storage :
-20°C
Description :
Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl - permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.
Immunogen :
Synthetic peptide derived from the C-terminal region of mouse Claudin-4
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1 µg/mL, Immunocytochemistry: Assay-dependent, Immunoprecipitation: 1-3 µg/mL, Western Blot: 1-3 µg/mL
Aliases :
Cep-r; claudin 4; CLAUDIN4; claudin-4; CLDN4; Clostridium perfringens enterotoxin receptor; Clostridium perfringens enterotoxin receptor 1; CPER; CPE-R; CPE-receptor; Cpetr; CPETR1; h CPE-receptor; hCPE-R; WBSCR8; Williams-Beuren syndrome chromosomal region 8 protein
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA