Prolactin Receptor Monoclonal Antibody (1A2B1) | Invitrogen 35-9200 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Prolactin Receptor Monoclonal Antibody (1A2B1)

catalog :

35-9200

quantity :

100 ug

price :

US 471.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1A2B1

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; loading ...; fig 4e	Alkharusi A, AlMuslahi A, AlBalushi N, AlAjmi R, AlRawahi S, AlFarqani A, et al. Connections between prolactin and ovarian cancer. PLoS ONE. 2021;16:e0255701 pubmed publisher
western blot; human; loading ...; fig 3a	Andreev J, Thambi N, Perez Bay A, Delfino F, Martin J, Kelly M, et al. Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs. Mol Cancer Ther. 2017;16:681-693 pubmed publisher
immunohistochemistry; human; loading ...; fig 1d	Tang M, Reedquist K, Garcia S, Fernandez B, Codullo V, Vieira Sousa E, et al. The prolactin receptor is expressed in rheumatoid arthritis and psoriatic arthritis synovial tissue and contributes to macrophage activation. Rheumatology (Oxford). 2016;55:2248-2259 pubmed
immunohistochemistry - paraffin section; human; loading ...; fig 1a	Chakhtoura Z, Laki F, Bernadet M, Cherifi I, Chiche A, Pigat N, et al. Gain-of-function Prolactin Receptor Variants Are Not Associated With Breast Cancer and Multiple Fibroadenoma Risk. J Clin Endocrinol Metab. 2016;101:4449-4460 pubmed
western blot; human; loading ...; fig 1g	Barcus C, Keely P, Eliceiri K, Schuler L. Prolactin signaling through focal adhesion complexes is amplified by stiff extracellular matrices in breast cancer cells. Oncotarget. 2016;7:48093-48106 pubmed publisher
flow cytometry; human; loading ...; fig 2d	Legorreta Haquet M, ChÃ¡vez Rueda K, ChÃ¡vez SÃ¡nchez L, Cervera Castillo H, Zenteno Galindo E, Barile Fabris L, et al. Function of Treg Cells Decreased in Patients With Systemic Lupus Erythematosus Due To the Effect of Prolactin. Medicine (Baltimore). 2016;95:e2384 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 4 immunocytochemistry; human; fig 3 western blot; mouse; fig 1	Alkharusi A, Lesma E, Ancona S, Chiaramonte E, Nystrom T, Gorio A, et al. Role of Prolactin Receptors in Lymphangioleiomyomatosis. PLoS ONE. 2016;11:e0146653 pubmed publisher
immunohistochemistry; human; 1:100; loading ...; fig 1a	Sutherland A, Forsyth A, Cong Y, Grant L, Juan T, Lee J, et al. The Role of Prolactin in Bone Metastasis and Breast Cancer Cell-Mediated Osteoclast Differentiation. J Natl Cancer Inst. 2016;108: pubmed publisher
western blot; human; 1:1000; fig 3	Zhang C, Nygaard M, Haxholm G, Boutillon F, Bernadet M, Hoos S, et al. A Residue Quartet in the Extracellular Domain of the Prolactin Receptor Selectively Controls Mitogen-activated Protein Kinase Signaling. J Biol Chem. 2015;290:11890-904 pubmed publisher
western blot; human	Barcus C, Holt E, Keely P, Eliceiri K, Schuler L. Dense collagen-I matrices enhance pro-tumorigenic estrogen-prolactin crosstalk in MCF-7 and T47D breast cancer cells. PLoS ONE. 2015;10:e0116891 pubmed publisher
western blot; human; 1:1000	Huang K, Tan D, Chen K, Walker A. Blockade of estrogen-stimulated proliferation by a constitutively-active prolactin receptor having lower expression in invasive ductal carcinoma. Cancer Lett. 2015;358:152-60 pubmed publisher
western blot; human; fig 4	Nitze L, Galsgaard E, Din N, Lund V, Rasmussen B, Berchtold M, et al. Reevaluation of the proposed autocrine proliferative function of prolactin in breast cancer. Breast Cancer Res Treat. 2013;142:31-44 pubmed publisher
western blot; human	Barcus C, Keely P, Eliceiri K, Schuler L. Stiff collagen matrices increase tumorigenic prolactin signaling in breast cancer cells. J Biol Chem. 2013;288:12722-32 pubmed publisher
immunohistochemistry; human; fig 6	Reuwer A, Nowak Sliwinska P, Mans L, van der Loos C, von der Thüsen J, Twickler M, et al. Functional consequences of prolactin signalling in endothelial cells: a potential link with angiogenesis in pathophysiology?. J Cell Mol Med. 2012;16:2035-48 pubmed publisher
western blot; human; 1:1000; fig 1	Tan D, Chen K, Khoo T, Walker A. Prolactin increases survival and migration of ovarian cancer cells: importance of prolactin receptor type and therapeutic potential of S179D and G129R receptor antagonists. Cancer Lett. 2011;310:101-8 pubmed publisher
immunohistochemistry; human; 1:500; fig 2	Liu W, Brooks C. Functional impact of manipulation on the relative orientation of human prolactin receptor domains. Biochemistry. 2011;50:5333-44 pubmed publisher
immunohistochemistry - paraffin section; human; 1:500; fig 2	Reuwer A, van Eijk M, Houttuijn Bloemendaal F, van der Loos C, Claessen N, Teeling P, et al. The prolactin receptor is expressed in macrophages within human carotid atherosclerotic plaques: a role for prolactin in atherogenesis?. J Endocrinol. 2011;208:107-17 pubmed publisher
western blot; human; fig 17.2	Goffin V, Bogorad R, Touraine P. Identification of gain-of-function variants of the human prolactin receptor. Methods Enzymol. 2010;484:329-55 pubmed publisher
western blot; human; fig 1	Rasmussen L, Frederiksen K, Din N, Galsgaard E, Christensen L, Berchtold M, et al. Prolactin and oestrogen synergistically regulate gene expression and proliferation of breast cancer cells. Endocr Relat Cancer. 2010;17:809-22 pubmed publisher
western blot; human	Carver K, Piazza T, Schuler L. Prolactin enhances insulin-like growth factor I receptor phosphorylation by decreasing its association with the tyrosine phosphatase SHP-2 in MCF-7 breast cancer cells. J Biol Chem. 2010;285:8003-12 pubmed publisher
western blot; human; fig 3	Varghese B, Barriere H, Carbone C, Banerjee A, Swaminathan G, Plotnikov A, et al. Polyubiquitination of prolactin receptor stimulates its internalization, postinternalization sorting, and degradation via the lysosomal pathway. Mol Cell Biol. 2008;28:5275-87 pubmed publisher
immunoprecipitation; human; fig 3 western blot; human; fig 3	Ueda E, Ozerdem U, Chen Y, Yao M, Huang K, Sun H, et al. A molecular mimic demonstrates that phosphorylated human prolactin is a potent anti-angiogenic hormone. Endocr Relat Cancer. 2006;13:95-111 pubmed
western blot; human; 1:2000; fig 5	Gutzman J, Miller K, Schuler L. Endogenous human prolactin and not exogenous human prolactin induces estrogen receptor alpha and prolactin receptor expression and increases estrogen responsiveness in breast cancer cells. J Steroid Biochem Mol Biol. 2004;88:69-77 pubmed
western blot; human; 1:2000; fig 4	Schroeder M, Brockman J, Walker A, Schuler L. Inhibition of prolactin (PRL)-induced proliferative signals in breast cancer cells by a molecular mimic of phosphorylated PRL, S179D-PRL. Endocrinology. 2003;144:5300-7 pubmed
	Grible J, Zot P, Olex A, Hedrick S, Harrell J, Woock A, et al. The human intermediate prolactin receptor is a mammary proto-oncogene. NPJ Breast Cancer. 2021;7:37 pubmed publisher
	Nie H, Huang P, Jiang S, Yang Q, Hu L, Yang X, et al. The short isoform of PRLR suppresses the pentose phosphate pathway and nucleotide synthesis through the NEK9-Hippo axis in pancreatic cancer. Theranostics. 2021;11:3898-3915 pubmed publisher
	Cayre S, Faraldo M, Bardin S, Miserey Lenkei S, Deugnier M, Goud B. RAB6 GTPase regulates mammary secretory function by controlling the activation of STAT5. Development. 2020;147: pubmed publisher
	Alkharusi A, Yu S, LandÃ¡zuri N, Zadjali F, Davodi B, NystrÃ¶m T, et al. Stimulation of prolactin receptor induces STAT-5 phosphorylation and cellular invasion in glioblastoma multiforme. Oncotarget. 2016;7:79572-79583 pubmed publisher
	Faupel Badger J, Duggan M, Sherman M, Garcia Closas M, Yang X, Lissowska J, et al. Prolactin receptor expression and breast cancer: relationships with tumor characteristics among pre- and post-menopausal women in a population-based case-control study from Poland. Horm Cancer. 2014;5:42-50 pubmed publisher
	Fleming J, Ginsburg E, McAndrew C, Heger C, Cheston L, Rodriguez Canales J, et al. Characterization of ?7/11, a functional prolactin-binding protein. J Mol Endocrinol. 2013;50:79-90 pubmed publisher
	Bellocci M, Sala G, Callegari F, Rossini G. Azaspiracid-1 inhibits endocytosis of plasma membrane proteins in epithelial cells. Toxicol Sci. 2010;117:109-21 pubmed publisher
	Galsgaard E, Rasmussen B, Folkesson C, Rasmussen L, Berchtold M, Christensen L, et al. Re-evaluation of the prolactin receptor expression in human breast cancer. J Endocrinol. 2009;201:115-28 pubmed publisher
	Bogorad R, Courtillot C, Mestayer C, Bernichtein S, Harutyunyan L, Jomain J, et al. Identification of a gain-of-function mutation of the prolactin receptor in women with benign breast tumors. Proc Natl Acad Sci U S A. 2008;105:14533-8 pubmed publisher
	Fang F, Antico G, Zheng J, Clevenger C. Quantification of PRL/Stat5 signaling with a novel pGL4-CISH reporter. BMC Biotechnol. 2008;8:11 pubmed publisher
	Lu J, Piazza T, Schuler L. Proteasomes mediate prolactin-induced receptor down-regulation and fragment generation in breast cancer cells. J Biol Chem. 2005;280:33909-16 pubmed

product information

Product Type :

Antibody

Product Name :

Prolactin Receptor Monoclonal Antibody (1A2B1)

Catalog # :

35-9200

Quantity :

100 ug

Price :

US 471.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: 2 ug/mL, Immunohistochemistry: 5-10 ug/mL, Immunoprecipitation: 5-10 ug/mL, Western Blot: 1-3 ug/mL

Species :

Human

Clone :

1A2B1

Isotype :

IgG2b, kappa

Storage :

-20 C

Description :

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Immunogen :

Recombinant human prolactin receptor protein containing the extracellular domain (ECD).

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 ug/mL, Immunohistochemistry: 5-10 ug/mL, Immunoprecipitation: 5-10 ug/mL, Western Blot: 1-3 ug/mL

Aliases :

AI987712; cytokine receptor; HPRL; hPRL receptor; hPRLrI; Lactogen receptor; MFAB; pPRLR-SF; Pr-1; Pr-3; Prl R; PRLR; PRL-R; Prlr-rs1; Prolactin receptor; prolactin receptor 2 precursor; prolactin receptor related sequence 1; prolactin receptor short isoform; RATPRLR; secreted prolactin binding protein

more info or order :

Invitrogen product webpage