Connexin 32 Monoclonal Antibody (5F9A9) | Invitrogen 35-8900 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Connexin 32 Monoclonal Antibody (5F9A9)

catalog :

35-8900

quantity :

100 ug

price :

US 471.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5F9A9

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig 2, 3 immunocytochemistry; mouse; 1:800; fig 4	Fowler S, McLean A, Bennett S. Tissue-specific cross-reactivity of connexin32 antibodies: problems and solutions unique to the central nervous system. Cell Commun Adhes. 2009;16:117-30 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 5a	Yoshikawa F, Sato Y, Tohyama K, Akagi T, Furuse T, Sadakata T, et al. Mammalian-Specific Central Myelin Protein Opalin Is Redundant for Normal Myelination: Structural and Behavioral Assessments. PLoS ONE. 2016;11:e0166732 pubmed publisher
western blot; mouse; fig 9	Park W, Kim S, Kim Y, Park J. Bortezomib alleviates drug-induced liver injury by regulating CYP2E1 gene transcription. Int J Mol Med. 2016;37:613-22 pubmed publisher
immunoprecipitation; human; fig 8b immunocytochemistry; human; fig 8c western blot; human; fig 8a	Kopanic J, Schlingmann B, Koval M, Lau A, Sorgen P, Su V. Degradation of gap junction connexins is regulated by the interaction with Cx43-interacting protein of 75 kDa (CIP75). Biochem J. 2015;466:571-85 pubmed publisher
western blot; mouse; 1:500	Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher
immunohistochemistry; mouse; 1:50; fig 2	Markoullis K, Sargiannidou I, Gardner C, Hadjisavvas A, Reynolds R, Kleopa K. Disruption of oligodendrocyte gap junctions in experimental autoimmune encephalomyelitis. Glia. 2012;60:1053-66 pubmed publisher
immunohistochemistry - frozen section; mouse; 2 ug/ml	Lynn B, Tress O, May D, Willecke K, Nagy J. Ablation of connexin30 in transgenic mice alters expression patterns of connexin26 and connexin32 in glial cells and leptomeninges. Eur J Neurosci. 2011;34:1783-93 pubmed publisher
immunocytochemistry; mouse; 1:500; fig 3	Degen J, Schütz M, Dicke N, Strenzke N, Jokwitz M, Moser T, et al. Connexin32 can restore hearing in connexin26 deficient mice. Eur J Cell Biol. 2011;90:817-24 pubmed publisher
western blot; mouse; 1:250; fig 7 immunocytochemistry; rat; 1:100 western blot; rat; 1:250	Yamaji S, Droggiti A, Lu S, Martinez Chantar M, Warner A, Varela Rey M. S-Adenosylmethionine regulates connexins sub-types expressed by hepatocytes. Eur J Cell Biol. 2011;90:312-22 pubmed publisher
immunohistochemistry; rat; 2 ug/ml; fig 3	Rash J, Olson C, Davidson K, Yasumura T, Kamasawa N, Nagy J. Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus. Neuroscience. 2007;147:938-56 pubmed
immunohistochemistry - frozen section; mouse; 1:400 immunohistochemistry; mouse western blot; mouse	Nagy J, Ionescu A, Lynn B, Rash J. Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice. Glia. 2003;44:205-18 pubmed
	Chang K, Agrawal I, Vainshtein A, Ho W, Xin W, Tucker Kellogg G, et al. TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells. elife. 2021;10: pubmed publisher
	Kim E, Bae Y, Choi M, Hong S. Connexin 43 plays an important role in the transformation of cholangiocytes with Clonochis sinensis excretory-secretory protein and N-nitrosodimethylamine. PLoS Negl Trop Dis. 2019;13:e0006843 pubmed publisher
	Jarjour A, Sherman D. Teasing of Ventral Spinal Cord White Matter Fibers for the Analysis of Central Nervous System Nodes of Ranvier. Methods Mol Biol. 2019;1936:129-139 pubmed publisher
	Coulibaly A, Isaacson L. Increased Cx32 expression in spinal cord TrkB oligodendrocytes following peripheral axon injury. Neurosci Lett. 2016;627:115-20 pubmed publisher
	Li X, Penes M, Odermatt B, Willecke K, Nagy J. Ablation of Cx47 in transgenic mice leads to the loss of MUPP1, ZONAB and multiple connexins at oligodendrocyte-astrocyte gap junctions. Eur J Neurosci. 2008;28:1503-17 pubmed publisher
	Kamasawa N, Sik A, Morita M, Yasumura T, Davidson K, Nagy J, et al. Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning. Neuroscience. 2005;136:65-86 pubmed
	Penes M, Li X, Nagy J. Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain. Eur J Neurosci. 2005;22:404-18 pubmed
	Nagy J, Ionescu A, Lynn B, Rash J. Connexin29 and connexin32 at oligodendrocyte and astrocyte gap junctions and in myelin of the mouse central nervous system. J Comp Neurol. 2003;464:356-70 pubmed

product information

Product Type :

Antibody

Product Name :

Connexin 32 Monoclonal Antibody (5F9A9)

Catalog # :

35-8900

Quantity :

100 ug

Price :

US 471.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Mouse, Rat

Applications :

Immunohistochemistry (Frozen): Assay-dependent, Western Blot: Assay-dependent

Species :

Mouse, Rat

Clone :

5F9A9

Isotype :

IgG2a

Storage :

-20 C

Description :

This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Immunogen :

Synthetic peptide derived from the C-terminus of mouse Connexin 32.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Frozen): Assay-dependent, Western Blot: Assay-dependent

Aliases :

CMTX; CMTX1; Cnx32; connexin 32; connexin-32; CX32; Cxn-32; GAP junction 28 kDa liver protein; gap junction beta-1 protein; gap junction membrane channel protein beta 1; gap junction protein beta 1; gap junction protein, beta 1; gap junction protein, beta 1, 32kDa; Gjb1

more info or order :

Invitrogen product webpage