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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CXCR4 Monoclonal Antibody (12G5)
catalog :
35-8800
quantity :
100 µg
price :
US 500
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12G5
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 44
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 2 ug/ml; loading ...; fig 2b
Nakamura Y, Kinoshita J, Yamaguchi T, Aoki T, Saito H, Hamabe Horiike T, et al. Crosstalk between cancer-associated fibroblasts and immune cells in peritoneal metastasis: inhibition in the migration of M2 macrophages and mast cells by Tranilast. Gastric Cancer. 2022;25:515-526 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 5f
Matsuzawa F, Kamachi H, Mizukami T, Einama T, Kawamata F, Fujii Y, et al. Mesothelin blockage by Amatuximab suppresses cell invasiveness, enhances gemcitabine sensitivity and regulates cancer cell stemness in mesothelin-positive pancreatic cancer cells. BMC Cancer. 2021;21:200 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Lim S, Kim J, Jeon S, Shin M, Kwon J, Kim T, et al. Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients. Front Immunol. 2019;10:496 pubmed publisher
  • flow cytometry; human; loading ...; fig 2d
Seki A, Rutz S. Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells. J Exp Med. 2018;215:985-997 pubmed publisher
  • flow cytometry; human; loading ...; fig 2e
Strazza M, Azoulay Alfaguter I, Peled M, Smrcka A, Skolnik E, Srivastava S, et al. PLCε1 regulates SDF-1α-induced lymphocyte adhesion and migration to sites of inflammation. Proc Natl Acad Sci U S A. 2017;114:2693-2698 pubmed publisher
  • flow cytometry; human; loading ...; tbl 2
Hajjar I, Goldstein F, Waller E, Moss L, Quyyumi A. Circulating Progenitor Cells is Linked to Cognitive Decline in Healthy Adults. Am J Med Sci. 2016;351:147-52 pubmed publisher
  • immunocytochemistry; mouse; fig 7
  • immunocytochemistry; human; loading ...; fig 7
McCutcheon S, Unachukwu U, Thakur A, MAJESKA R, Redenti S, Vazquez M. In vitro formation of neuroclusters in microfluidic devices and cell migration as a function of stromal-derived growth factor 1 gradients. Cell Adh Migr. 2017;11:1-12 pubmed publisher
  • immunocytochemistry; human; 5 ug/ml; loading ...; fig 2b
Rico Varela J, Singh T, McCutcheon S, Vazquez M. EGF as a New Therapeutic Target for Medulloblastoma Metastasis. Cell Mol Bioeng. 2015;8:553-565 pubmed
  • immunohistochemistry; human; 1:25
Winczura P, Sosińska Mielcarek K, Duchnowska R, Badzio A, Lakomy J, Majewska H, et al. Immunohistochemical Predictors of Bone Metastases in Breast Cancer Patients. Pathol Oncol Res. 2015;21:1229-36 pubmed publisher
  • immunocytochemistry; human
van Gijsel Bonnello M, Acar N, Molino Y, Bretillon L, Khrestchatisky M, De Reggi M, et al. Pantethine Alters Lipid Composition and Cholesterol Content of Membrane Rafts, With Down-Regulation of CXCL12-Induced T Cell Migration. J Cell Physiol. 2015;230:2415-25 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Mizukami T, Kamachi H, Mitsuhashi T, Tsuruga Y, Hatanaka Y, Kamiyama T, et al. Immunohistochemical analysis of cancer stem cell markers in pancreatic adenocarcinoma patients after neoadjuvant chemoradiotherapy. BMC Cancer. 2014;14:687 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1200
Wyler L, Napoli C, Ingold B, Sulser T, Heikenwalder M, Schraml P, et al. Brain metastasis in renal cancer patients: metastatic pattern, tumour-associated macrophages and chemokine/chemoreceptor expression. Br J Cancer. 2014;110:686-94 pubmed publisher
  • immunohistochemistry; human; 1:25; tbl 3
Sosińska Mielcarek K, Duchnowska R, Winczura P, Badzio A, Majewska H, Lakomy J, et al. Immunohistochemical prediction of brain metastases in patients with advanced breast cancer: the role of Rad51. Breast. 2013;22:1178-83 pubmed publisher
  • flow cytometry; human; fig 1
Perdomo Arciniegas A, Vernot J. Co-culture of hematopoietic stem cells with mesenchymal stem cells increases VCAM-1-dependent migration of primitive hematopoietic stem cells. Int J Hematol. 2011;94:525-32 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; tbl 1
Welsch T, Keleg S, Bergmann F, Degrate L, Bauer S, Schmidt J. Comparative analysis of tumorbiology and CD133 positivity in primary and recurrent pancreatic ductal adenocarcinoma. Clin Exp Metastasis. 2009;26:701-11 pubmed publisher
  • flow cytometry; human; loading ...
Lai L, Vödrös D, Kozlowski P, Montefiori D, Wilson R, Akerstrom V, et al. GM-CSF DNA: an adjuvant for higher avidity IgG, rectal IgA, and increased protection against the acute phase of a SHIV-89.6P challenge by a DNA/MVA immunodeficiency virus vaccine. Virology. 2007;369:153-67 pubmed
  • immunohistochemistry - paraffin section; human; 1:50; fig 5B
Sato N, Fukushima N, Maitra A, Iacobuzio Donahue C, Van Heek N, Cameron J, et al. Gene expression profiling identifies genes associated with invasive intraductal papillary mucinous neoplasms of the pancreas. Am J Pathol. 2004;164:903-14 pubmed
Gao M, Fu Y, Zhou W, Gui G, Lal B, Li Y, et al. EGFR Activates a TAZ-Driven Oncogenic Program in Glioblastoma. Cancer Res. 2021;81:3580-3592 pubmed publisher
Jang J, Thuy P, Lee J, Moon E. CXCR4 promotes B cell viability by the cooperation of nuclear factor (erythroid-derived 2)-like 2 and hypoxia-inducible factor-1α under hypoxic conditions. Cell Death Dis. 2021;12:330 pubmed publisher
Ren T, Wei P, Song Q, Ye Z, Wang Y, Huang L. MiR-140-3p Ameliorates the Progression of Osteoarthritis via Targeting CXCR4. Biol Pharm Bull. 2020;43:810-816 pubmed publisher
Wang G, Hu W, Chen H, Shou X, Ye T, Xu Y. Cocktail Strategy Based on NK Cell-Derived Exosomes and Their Biomimetic Nanoparticles for Dual Tumor Therapy. Cancers (Basel). 2019;11: pubmed publisher
Liu G, Johnson S, Jain R, Peeler D, Shankland S, Pun S. Optimized nonviral gene delivery for primary urinary renal progenitor cells to enhance cell migration. J Biomed Mater Res A. 2019;107:2718-2725 pubmed publisher
Itkin T, Gur Cohen S, Spencer J, Schajnovitz A, Ramasamy S, Kusumbe A, et al. Distinct bone marrow blood vessels differentially regulate haematopoiesis. Nature. 2016;532:323-8 pubmed publisher
Han A, Lee J, Kim H, Min W, Park G, Kim S. A CXCR4 antagonist leads to tumor suppression by activation of immune cells in a leukemia-induced microenvironment. Oncol Rep. 2015;34:2880-8 pubmed publisher
Pelekanos R, Ting M, Sardesai V, Ryan J, Lim Y, Chan J, et al. Intracellular trafficking and endocytosis of CXCR4 in fetal mesenchymal stem/stromal cells. BMC Cell Biol. 2014;15:15 pubmed publisher
Beider K, Darash Yahana M, Blaier O, Koren Michowitz M, Abraham M, Wald H, et al. Combination of imatinib with CXCR4 antagonist BKT140 overcomes the protective effect of stroma and targets CML in vitro and in vivo. Mol Cancer Ther. 2014;13:1155-69 pubmed publisher
Serrano Pertierra E, Cernuda Morollón E, Lopez Larrea C. NKG2D- and CD28-mediated costimulation regulate CD8+ T cell chemotaxis through different mechanisms: the role of Cdc42/N-WASp. J Leukoc Biol. 2014;95:487-95 pubmed publisher
Igarashi S, Sato Y, Ren X, Harada K, Sasaki M, Nakanuma Y. Participation of peribiliary glands in biliary tract pathophysiologies. World J Hepatol. 2013;5:425-32 pubmed publisher
Park B, Kook S, Lee S, Jeong J, Brufsky A, Lee B. An isoform of C/EBP?, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration. J Biol Chem. 2013;288:28656-67 pubmed publisher
Turaç G, Hindley C, Thomas R, Davis J, Deleidi M, Gasser T, et al. Combined flow cytometric analysis of surface and intracellular antigens reveals surface molecule markers of human neuropoiesis. PLoS ONE. 2013;8:e68519 pubmed publisher
Lu C, Guo J, Gu J, Ge D, Hou Y, Lin Z, et al. CXCR4 heterogeneous expression in esophageal squamous cell cancer and stronger metastatic potential with CXCR4-positive cancer cells. Dis Esophagus. 2014;27:294-302 pubmed publisher
Sison E, McIntyre E, Magoon D, Brown P. Dynamic chemotherapy-induced upregulation of CXCR4 expression: a mechanism of therapeutic resistance in pediatric AML. Mol Cancer Res. 2013;11:1004-16 pubmed publisher
Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, et al. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013;73:2598-607 pubmed publisher
Oh Y, Kim H, Song I, Yun H, Jo D, Kim S, et al. Hypoxia induces CXCR4 expression and biological activity in gastric cancer cells through activation of hypoxia-inducible factor-1?. Oncol Rep. 2012;28:2239-46 pubmed publisher
Prabakar K, Dominguez Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, et al. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant. 2012;21:1321-39 pubmed publisher
Zhang R, Pan X, Huang Z, Weber G, Zhang G. Osteopontin enhances the expression and activity of MMP-2 via the SDF-1/CXCR4 axis in hepatocellular carcinoma cell lines. PLoS ONE. 2011;6:e23831 pubmed publisher
Kwajah M M S, Schwarz H. CD137 ligand signaling induces human monocyte to dendritic cell differentiation. Eur J Immunol. 2010;40:1938-49 pubmed publisher
Zou D, Yoon H, Anjomshoaa A, Perez D, Fukuzawa R, Guilford P, et al. Increased levels of active c-Src distinguish invasive from in situ lobular lesions. Breast Cancer Res. 2009;11:R45 pubmed publisher
Huang E, Singh B, Cristofanilli M, Gelovani J, Wei C, Vincent L, et al. A CXCR4 antagonist CTCE-9908 inhibits primary tumor growth and metastasis of breast cancer. J Surg Res. 2009;155:231-6 pubmed publisher
Dubeykovskaya Z, Dubeykovskiy A, Solal Cohen J, Wang T. Secreted trefoil factor 2 activates the CXCR4 receptor in epithelial and lymphocytic cancer cell lines. J Biol Chem. 2009;284:3650-62 pubmed publisher
Mikami S, Nakase H, Yamamoto S, Takeda Y, Yoshino T, Kasahara K, et al. Blockade of CXCL12/CXCR4 axis ameliorates murine experimental colitis. J Pharmacol Exp Ther. 2008;327:383-92 pubmed publisher
Koenen H, Smeets R, Vink P, van Rijssen E, Boots A, Joosten I. Human CD25highFoxp3pos regulatory T cells differentiate into IL-17-producing cells. Blood. 2008;112:2340-52 pubmed publisher
Uchida D, Begum N, Tomizuka Y, Bando T, Almofti A, Yoshida H, et al. Acquisition of lymph node, but not distant metastatic potentials, by the overexpression of CXCR4 in human oral squamous cell carcinoma. Lab Invest. 2004;84:1538-46 pubmed
Endres M, Clapham P, Marsh M, Ahuja M, Turner J, McKnight A, et al. CD4-independent infection by HIV-2 is mediated by fusin/CXCR4. Cell. 1996;87:745-56 pubmed
product information
Product Type :
Antibody
Product Name :
CXCR4 Monoclonal Antibody (12G5)
Catalog # :
35-8800
Quantity :
100 µg
Price :
US 500
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 3-5 µg/mL, Immunofluorescence: Assay Dependent, Western Blot: 2 µg/mL
Species :
Human
Clone :
12G5
Isotype :
IgG2a, kappa
Storage :
-20°C
Description :
CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
Immunogen :
SIV-infected SupT1 cells injected into Balb/c mice.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 3-5 µg/mL, Immunofluorescence: Assay Dependent, Western Blot: 2 µg/mL
Aliases :
b2b220Clo; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C) receptor 4; chemokine receptor; chemokine receptor (LCR1); chemokine receptor 4; chemokine receptor CXCR4; Cmkar4; CXC; CXC chemokine receptor; C-X-C chemokine receptor type 4; CXC motif chemokine; C-X-C motif chemokine receptor 4; Cxcr4; CXC-R4; CXCR-4; D2S201E; FB22; Fusin; HM89; HSY3RR; HUMSTR; LAP3; LAP-3; LCR1; LESTR; leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPY3R; NPYR; NPYRL; NPYY3R; PB-CKR; PBSF/SDF-1; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; Sdf1r; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; stromal cell-derived factor 1 receptor; WHIM; WHIMS
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA