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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
COX2 Monoclonal Antibody (COX 229)
catalog :
35-8200
quantity :
100 ug
price :
US 471.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
COX 229
reactivity :
human, mouse, rat, domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 34
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse
Chew G, Huo C, Huang D, Hill P, Cawson J, Frazer H, et al. Increased COX-2 expression in epithelial and stromal cells of high mammographic density tissues and in a xenograft model of mammographic density. Breast Cancer Res Treat. 2015;153:89-99 pubmed publisher
  • western blot; human; 1:1000; fig 4
Lei Q, Qiang F, Chao D, Di W, Guoqian Z, Bo Y, et al. Amelioration of hypoxia and LPS-induced intestinal epithelial barrier dysfunction by emodin through the suppression of the NF-κB and HIF-1α signaling pathways. Int J Mol Med. 2014;34:1629-39 pubmed publisher
  • western blot; human; 1:1000
Bhat I, Rasool R, Qasim I, Masoodi K, Paul S, Bhat B, et al. COX-2 overexpression and -8473 T/C polymorphism in 3' UTR in non-small cell lung cancer. Tumour Biol. 2014;35:11209-18 pubmed publisher
  • western blot; rat; 1:500
Tapias V, Cannon J, Greenamyre J. Pomegranate juice exacerbates oxidative stress and nigrostriatal degeneration in Parkinson's disease. Neurobiol Aging. 2014;35:1162-76 pubmed publisher
  • western blot; human; fig 3
Jourdain A, Koppen M, Wydro M, Rodley C, Lightowlers R, Chrzanowska Lightowlers Z, et al. GRSF1 regulates RNA processing in mitochondrial RNA granules. Cell Metab. 2013;17:399-410 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
Taskin S, Dunder I, Erol E, Taşkin E, Kiremitci S, Oztuna D, et al. Roles of E-cadherin and cyclooxygenase enzymes in predicting different survival patterns of optimally cytoreduced serous ovarian cancer patients. Asian Pac J Cancer Prev. 2012;13:5715-9 pubmed
  • immunocytochemistry; human; fig 4
Lundström S, Levänen B, Nording M, Klepczynska Nyström A, Sköld M, Haeggstrom J, et al. Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure. PLoS ONE. 2011;6:e23864 pubmed publisher
  • immunohistochemistry - paraffin section; human; tbl 2
  • immunocytochemistry; human; tbl 1
Luo H, Chen Z, Jin H, Zhuang M, Wang T, Su C, et al. Cyclooxygenase-2 up-regulates vascular endothelial growth factor via a protein kinase C pathway in non-small cell lung cancer. J Exp Clin Cancer Res. 2011;30:6 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; fig 3
Shiirevnyamba A, Takahashi T, Shan H, Ogawa H, Yano S, Kanayama H, et al. Enhancement of osteoclastogenic activity in osteolytic prostate cancer cells by physical contact with osteoblasts. Br J Cancer. 2011;104:505-13 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 3
Lage K, Møllgård K, Greenway S, Wakimoto H, Gorham J, Workman C, et al. Dissecting spatio-temporal protein networks driving human heart development and related disorders. Mol Syst Biol. 2010;6:381 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:400
Maloney S, Fernandes B, Castiglione E, Antecka E, Martins C, Marshall J, et al. Expression of cyclooxygenase-2 in choroidal neovascular membranes from age-related macular degeneration patients. Retina. 2009;29:176-80 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; fig 1
Sackett M, Bairati I, Meyer F, Jobin E, Lussier S, Fortin A, et al. Prognostic significance of cyclooxygenase-2 overexpression in glottic cancer. Clin Cancer Res. 2008;14:67-73 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; tbl 1
Ketabchi S, Massi D, Ficarra G, Rubino I, Franchi A, Paglierani M, et al. Expression of protease-activated receptor-1 and -2 in orofacial granulomatosis. Oral Dis. 2007;13:419-25 pubmed
  • immunohistochemistry - paraffin section; human; fig 2
Buccoliero A, Castiglione F, Rossi Degl Innocenti D, Arganini L, Taddei A, Ammannati F, et al. Cyclooxygenase-2 (COX-2) overexpression in meningiomas: real time PCR and immunohistochemistry. Appl Immunohistochem Mol Morphol. 2007;15:187-92 pubmed
  • immunohistochemistry - paraffin section; human; 1:50; fig 1
Bianchini F, Massi D, Marconi C, Franchi A, Baroni G, Santucci M, et al. Expression of cyclo-oxygenase-2 in macrophages associated with cutaneous melanoma at different stages of progression. Prostaglandins Other Lipid Mediat. 2007;83:320-8 pubmed
  • immunohistochemistry - paraffin section; domestic rabbit; 1:500
Marshall J, Fernandes B, Di Cesare S, Maloney S, Logan P, Antecka E, et al. The use of a cyclooxygenase-2 inhibitor (Nepafenac) in an ocular and metastatic animal model of uveal melanoma. Carcinogenesis. 2007;28:2053-8 pubmed
  • immunohistochemistry - paraffin section; human; 1:100
Kim K, Kim S, Kim S, Back J, Park M, Kim J. Cyclooxygenase-2 and inducible nitric oxide synthase expression in thyroid neoplasms and their clinicopathological correlation. J Korean Med Sci. 2006;21:1064-9 pubmed
  • immunohistochemistry - paraffin section; human; 1:50; fig 1
Souza Filho J, Martins M, Correa Z, Odashiro A, Antecka E, Coutinho A, et al. The expression of cyclooxygenase 2 in retinoblastoma: primary enucleated eyes and enucleation after conservative treatment. Am J Ophthalmol. 2006;142:625-31 pubmed
  • immunohistochemistry - paraffin section; rat; 1:100; fig 2
Asaad N, Sadek G. Pulmonary cryptosporidiosis: role of COX2 and NF-kB. APMIS. 2006;114:682-9 pubmed
  • immunohistochemistry - paraffin section; human
Bodey B, Siegel S, Kaiser H. Cyclooxygenase-2 (COX-2) overexpression in childhood brain tumors. In Vivo. 2006;20:519-25 pubmed
Hu Y, Gu X, Zhang Y, Ma W, Sun L, Wang C, et al. Adrenomedullin, transcriptionally regulated by vitamin D receptors, alleviates atherosclerosis in mice through suppressing AMPK-mediated endothelial ferroptosis. Environ Toxicol. 2024;39:199-211 pubmed publisher
Han R, Wan J, Han X, Ren H, Falck J, Munnuri S, et al. 20-HETE Participates in Intracerebral Hemorrhage-Induced Acute Injury by Promoting Cell Ferroptosis. Front Neurol. 2021;12:763419 pubmed publisher
Chen Y, Wang C, Cheng C, Liu F, Lee C, Lee H, et al. Interleukin-26 Has Synergistic Catabolic Effects with Palmitate in Human Articular Chondrocytes via the TLR4-ERK1/2-c-Jun Signaling Pathway. Cells. 2021;10: pubmed publisher
Clemenceau A, Hanna M, Ennour Idrissi K, Burguin A, Diorio C, Durocher F. Secreted Frizzled-Related Protein 1 as a Biomarker against Incomplete Age-Related Lobular Involution and Microcalcifications' Development. Cancers (Basel). 2020;12: pubmed publisher
Lee J, You J, Kim M, Roh J. Epigenetic reprogramming of epithelial-mesenchymal transition promotes ferroptosis of head and neck cancer. Redox Biol. 2020;37:101697 pubmed publisher
Lee J, Han S, Park M, Song I, Choi M, Yu E, et al. N-AS-triggered SPMs are direct regulators of microglia in a model of Alzheimer's disease. Nat Commun. 2020;11:2358 pubmed publisher
Albouchi F, Avola R, Dico G, Calabrese V, Graziano A, Abderrabba M, et al. Melaleuca styphelioides Sm. Polyphenols Modulate Interferon Gamma/Histamine-Induced Inflammation in Human NCTC 2544 Keratinocytes. Molecules. 2018;23: pubmed publisher
Rosário Pinheiro D, Harada M, Rodríguez Burbano R, Nascimento Borges B. COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma. Genet Mol Biol. 2018;41:496-501 pubmed publisher
Qian Y, Song J, Sun P, Yi R, Liu H, Feng X, et al. Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells. Molecules. 2018;23: pubmed publisher
Hanna M, Dumas I, Orain M, Jacob S, Tetu B, Sanschagrin F, et al. Association between local inflammation and breast tissue age-related lobular involution among premenopausal and postmenopausal breast cancer patients. PLoS ONE. 2017;12:e0183579 pubmed publisher
Larouche D, Hanna M, Chang S, Jacob S, Tetu B, Diorio C. Evaluation of Antioxidant Intakes in Relation to Inflammatory Markers Expression Within the Normal Breast Tissue of Breast Cancer Patients. Integr Cancer Ther. 2017;16:485-495 pubmed publisher
Grau L, Luque Garcia J, Gonzalez Peramato P, Theodorescu D, Palou J, Fernández Gómez J, et al. A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness. PLoS ONE. 2013;8:e53328 pubmed publisher
Birkenkamp Demtroder K, Olesen S, Sørensen F, Laurberg S, Laiho P, Aaltonen L, et al. Differential gene expression in colon cancer of the caecum versus the sigmoid and rectosigmoid. Gut. 2005;54:374-84 pubmed
Johnsen J, Lindskog M, Ponthan F, Pettersen I, Elfman L, Orrego A, et al. Cyclooxygenase-2 is expressed in neuroblastoma, and nonsteroidal anti-inflammatory drugs induce apoptosis and inhibit tumor growth in vivo. Cancer Res. 2004;64:7210-5 pubmed
product information
Product Type :
Antibody
Product Name :
COX2 Monoclonal Antibody (COX 229)
Catalog # :
35-8200
Quantity :
100 ug
Price :
US 471.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
ELISA: Assay-dependent, Immunocytochemistry: 2 ug/mL, Immunohistochemistry: Assay-dependent, Western Blot: 1 ug/mL
Species :
Human, Mouse
Clone :
COX 229
Isotype :
IgG1, kappa
Storage :
-20 C
Description :
COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.
Immunogen :
Synthetic peptide from human COX-2
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunocytochemistry: 2 ug/mL, Immunohistochemistry: Assay-dependent, Western Blot: 1 ug/mL
Aliases :
COII; COX2; Cox-2; COXII; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2b; cyclooxygenase-2; Cytochrome c oxidase polypeptide II; Cytochrome c oxidase subunit 2; cytochrome c oxidase subunit II; glucocorticoid-regulated inflammatory cyclooxygenase; Gripghs; hCox-2; Macrophage activation-associated marker protein P71/73; mitochondrially encoded cytochrome c oxidase II; MTCO2; MT-CO2; PES-2; PGG/HS; PGH synthase 2; Pghs2; PGHS-2; Pghs-b; PHS II; PHS-2; prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Ptgs2; Tis10; TIS10 protein
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA