PKP3 Monoclonal Antibody (23E3/4) | Invitrogen 35-7600 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PKP3 Monoclonal Antibody (23E3/4)

catalog :

35-7600

quantity :

100 µg

price :

425 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

23E3/4

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000	Basu S, Thorat R, Dalal S. MMP7 is required to mediate cell invasion and tumor formation upon Plakophilin3 loss. PLoS ONE. 2015;10:e0123979 pubmed publisher
immunocytochemistry; mouse; 1:250; fig 1	Baron S, Hoang A, Vogel H, Attardi L. Unimpaired skin carcinogenesis in Desmoglein 3 knockout mice. PLoS ONE. 2012;7:e50024 pubmed publisher
western blot; human; 1:1000	Khapare N, Kundu S, Sehgal L, Sawant M, Priya R, Gosavi P, et al. Plakophilin3 loss leads to an increase in PRL3 levels promoting K8 dephosphorylation, which is required for transformation and metastasis. PLoS ONE. 2012;7:e38561 pubmed publisher
immunocytochemistry; human; 1:25; fig 5 western blot; human; 1:1000; fig 2	Gosavi P, Kundu S, Khapare N, Sehgal L, Karkhanis M, Dalal S. E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly. Cell Mol Life Sci. 2011;68:1439-54 pubmed publisher
chromatin immunoprecipitation; human; fig 3 immunocytochemistry; human; fig 2 immunohistochemistry; human; fig 5 western blot; human; fig 1	Aigner K, Descovich L, Mikula M, Sultan A, Dampier B, Bonne S, et al. The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression. FEBS Lett. 2007;581:1617-24 pubmed
	Ehmsen S, Pedersen M, Wang G, Terp M, Arslanagic A, Hood B, et al. Increased Cholesterol Biosynthesis Is a Key Characteristic of Breast Cancer Stem Cells Influencing Patient Outcome. Cell Rep. 2019;27:3927-3938.e6 pubmed publisher
	Furukawa C, Daigo Y, Ishikawa N, Kato T, Ito T, Tsuchiya E, et al. Plakophilin 3 oncogene as prognostic marker and therapeutic target for lung cancer. Cancer Res. 2005;65:7102-10 pubmed

product information

Product Type :

Antibody

Product Name :

PKP3 Monoclonal Antibody (23E3/4)

Catalog # :

35-7600

Quantity :

100 µg

Price :

425 USD

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: 1-3 µg/mL

Species :

Human

Clone :

23E3/4

Isotype :

IgG2b

Storage :

-20°C

Description :

Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalization, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localization in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of ~87 kDa.

Immunogen :

Synthetic peptide from Armadillo domain of human Plakophilin-3.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: 1-3 µg/mL

Aliases :

2310056L12Rik; Pkp3; plakophilin 3; plakophilin 3b; plakophilin-3

more info or order :

Invitrogen product webpage