Phospho-Tau (Ser396) Monoclonal Antibody (PHF13.6) | Invitrogen 35-5300 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Tau (Ser396) Monoclonal Antibody (PHF13.6)

catalog :

35-5300

quantity :

50 µg

price :

US 375.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

PHF13.6

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; dogs; 1:1000 western blot; mouse western blot; rat	Smolek T, Madari A, Farbáková J, Kandrac O, Jadhav S, Cente M, et al. Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment. J Comp Neurol. 2016;524:874-95 pubmed publisher
western blot; human; 1:1000	Loeffler D, Smith L, Klaver A, MartiÄ‡ S. Effects of antibodies to phosphorylated and non-phosphorylated tau on in vitro tau phosphorylation at Serine-199: Preliminary report. Exp Gerontol. 2015;67:15-8 pubmed publisher
western blot; human; fig 3, 4	Sepúlveda Díaz J, Alavi Naini S, Huynh M, Ouidja M, Yanicostas C, Chantepie S, et al. HS3ST2 expression is critical for the abnormal phosphorylation of tau in Alzheimer's disease-related tau pathology. Brain. 2015;138:1339-54 pubmed publisher
western blot; rat; 1:1000; fig 1	Li R, Xu D, Ma T. Lovastatin suppresses the aberrant tau phosphorylation from FTDP-17 mutation and okadaic acid-induction in rat primary neurons. Neuroscience. 2015;294:14-20 pubmed publisher
western blot; rat; fig 3	Å polcovÃ¡ A, MikulÃ¡Å¡kovÃ¡ B, KrÅ¡kovÃ¡ K, GajdoÅ¡echovÃ¡ L, ZÃ³rad Å, Olszanecki R, et al. Deficient hippocampal insulin signaling and augmented Tau phosphorylation is related to obesity- and age-induced peripheral insulin resistance: a study in Zucker rats. BMC Neurosci. 2014;15:111 pubmed publisher
western blot; human; 1:500	Spatara M, Robinson A. Transgenic mouse and cell culture models demonstrate a lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction. J Neurosci Res. 2010;88:1951-61 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200	Forno L, Langston J, Herrick M, Wilson J, Murayama S. Ubiquitin-positive neuronal and tau 2-positive glial inclusions in frontotemporal dementia of motor neuron type. Acta Neuropathol. 2002;103:599-606 pubmed
	Yu F, Shukla D, Armstrong R, Marion C, Radomski K, Selwyn R, et al. Repetitive Model of Mild Traumatic Brain Injury Produces Cortical Abnormalities Detectable by Magnetic Resonance Diffusion Imaging, Histopathology, and Behavior. J Neurotrauma. 2017;34:1364-1381 pubmed publisher
	Hoffmann R, Lee V, Leight S, Varga I, Otvos L. Unique Alzheimer's disease paired helical filament specific epitopes involve double phosphorylation at specific sites. Biochemistry. 1997;36:8114-24 pubmed

product information

Product Type :

Antibody

Product Name :

Phospho-Tau (Ser396) Monoclonal Antibody (PHF13.6)

Catalog # :

35-5300

Quantity :

50 µg

Price :

US 375.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 3-5 µg/mL, Western Blot: 1-3 µg/mL

Species :

Human, Mouse, Rat

Clone :

PHF13.6

Isotype :

IgG2b

Storage :

-20°C

Description :

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen :

Purified human PHF-Tau preparation

Format :

Liquid

Applications w/Dilutions :

ELISA: 3-5 µg/mL, Western Blot: 1-3 µg/mL

Aliases :

AI413597; AW045860; DDPAC; FLJ31424; FTDP17; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; Mapt; MAPTL; MGC138549; microtubule associated protein tau; microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; Tau; Tau microtubule-associated protein; tau protein; Tau-4; Tau5; Unknown (protein for MGC:134287)

more info or order :

Invitrogen product webpage