Connexin 43 Monoclonal Antibody (3D8A5) | Invitrogen 35-5000 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Connexin 43 Monoclonal Antibody (3D8A5)

catalog :

35-5000

quantity :

100 ug

price :

US 451.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3D8A5

reactivity :

human, mouse, rat, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:100; loading ...; fig 1b	Theofilas P, Steinhäuser C, Theis M, Derouiche A. Morphological study of a connexin 43-GFP reporter mouse highlights glial heterogeneity, amacrine cells, and olfactory ensheathing cells. J Neurosci Res. 2017;95:2182-2194 pubmed publisher
western blot; domestic rabbit; tbl 2	Chen X, Lu S, Huang K, Zhang W, Liu Z, Zhong J. Effects of Ang â…¡ perfusion on transmural heterogeneous of Cx43 in acute myocardial ischemia reperfusion. Asian Pac J Trop Med. 2016;9:96-9 pubmed publisher
western blot; mouse	Kundu S, Pushpakumar S, Sen U. MMP-9- and NMDA receptor-mediated mechanism of diabetic renovascular remodeling and kidney dysfunction: hydrogen sulfide is a key modulator. Nitric Oxide. 2015;46:172-85 pubmed publisher
immunocytochemistry; rat immunocytochemistry; mouse	Bautista W, Rash J, Vanderpool K, Yasumura T, Nagy J. Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus. Eur J Neurosci. 2014;39:757-70 pubmed publisher
immunohistochemistry - frozen section; human; 1:200; fig 3 immunohistochemistry - frozen section; rat; 1:200; fig 2 immunohistochemistry - frozen section; mouse; 1:200; fig 1	Liang B, Nissen J, Laursen M, Wang X, Skibsbye L, Hearing M, et al. G-protein-coupled inward rectifier potassium current contributes to ventricular repolarization. Cardiovasc Res. 2014;101:175-84 pubmed publisher
immunohistochemistry - frozen section; mouse; 2 ug/ml	Lynn B, Tress O, May D, Willecke K, Nagy J. Ablation of connexin30 in transgenic mice alters expression patterns of connexin26 and connexin32 in glial cells and leptomeninges. Eur J Neurosci. 2011;34:1783-93 pubmed publisher
western blot; rat; fig 3	Takahashi D, Vargas J, Wilcox K. Increased coupling and altered glutamate transport currents in astrocytes following kainic-acid-induced status epilepticus. Neurobiol Dis. 2010;40:573-85 pubmed publisher
immunohistochemistry; rat	Procida K, Jørgensen L, Schmitt N, Delmar M, Taffet S, Holstein Rathlou N, et al. Phosphorylation of connexin43 on serine 306 regulates electrical coupling. Heart Rhythm. 2009;6:1632-8 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 3	ElZarrad M, Haroon A, Willecke K, Dobrowolski R, Gillespie M, Al Mehdi A. Connexin-43 upregulation in micrometastases and tumor vasculature and its role in tumor cell attachment to pulmonary endothelium. BMC Med. 2008;6:20 pubmed publisher
immunocytochemistry; rat; 1:500; fig 2	Sorensen C, Salomonsson M, Braunstein T, Nielsen M, Holstein Rathlou N. Connexin mimetic peptides fail to inhibit vascular conducted calcium responses in renal arterioles. Am J Physiol Regul Integr Comp Physiol. 2008;295:R840-7 pubmed publisher
immunohistochemistry - frozen section; mouse	Leaf D, Feig J, Vasquez C, Riva P, Yu C, Lader J, et al. Connexin40 imparts conduction heterogeneity to atrial tissue. Circ Res. 2008;103:1001-8 pubmed publisher
immunocytochemistry; rat; fig 2	Kang J, Kang N, Lovatt D, Torres A, Zhao Z, Lin J, et al. Connexin 43 hemichannels are permeable to ATP. J Neurosci. 2008;28:4702-11 pubmed publisher
immunohistochemistry; rat; 2 ug/ml; fig 3	Rash J, Olson C, Davidson K, Yasumura T, Kamasawa N, Nagy J. Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus. Neuroscience. 2007;147:938-56 pubmed
western blot; domestic rabbit; 1:1000; fig 3	Tansey E, Kwaku K, Hammer P, Cowan D, Federman M, Levitsky S, et al. Reduction and redistribution of gap and adherens junction proteins after ischemia and reperfusion. Ann Thorac Surg. 2006;82:1472-9 pubmed
immunohistochemistry - frozen section; human; fig 5	Van Der Aa F, Roskams T, Blyweert W, Ost D, Bogaert G, De Ridder D. Identification of kit positive cells in the human urinary tract. J Urol. 2004;171:2492-6 pubmed
immunohistochemistry - frozen section; mouse	Nagy J, Ionescu A, Lynn B, Rash J. Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice. Glia. 2003;44:205-18 pubmed
	Wu I, Zeng A, Greer Short A, Aycinena J, Tefera A, Shenwai R, et al. AAV9:PKP2 improves heart function and survival in a Pkp2-deficient mouse model of arrhythmogenic right ventricular cardiomyopathy. Commun Med (Lond). 2024;4:38 pubmed publisher
	Misrani A, Ngwa C, Mamun A, Sharmeen R, Manyam K, Ritzel R, et al. Brain endothelial CD200 signaling protects brain against ischemic damage. Brain Res Bull. 2024;207:110864 pubmed publisher
	Mastrototaro G, Carullo P, Zhang J, Scellini B, Piroddi N, Nemska S, et al. Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities. elife. 2023;12: pubmed publisher
	Afzal J, Liu Y, Du W, Suhail Y, Zong P, Feng J, et al. Cardiac ultrastructure inspired matrix induces advanced metabolic and functional maturation of differentiated human cardiomyocytes. Cell Rep. 2022;40:111146 pubmed publisher
	Pang J, Thomas N, Tsuchiya D, Parmely T, Yan D, Xie T, et al. Step-by-step preparation of mouse eye sections for routine histology, immunofluorescence, and RNA in situ hybridization multiplexing. STAR Protoc. 2021;2:100879 pubmed publisher
	Godinho Pereira J, Garcia A, Figueira I, Malh xf3 R, Brito M. Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption. Int J Mol Sci. 2021;22: pubmed publisher
	Pang J, Le L, Zhou Y, Tu R, Hou Q, Tsuchiya D, et al. NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression. Cell Rep. 2021;34:108603 pubmed publisher
	Veeranki S, Tyagi S. Mdivi-1 induced acute changes in the angiogenic profile after ischemia-reperfusion injury in female mice. Physiol Rep. 2017;5: pubmed publisher
	Tsao D, Wang S, Lynn B, Nagy J. Immunofluorescence reveals unusual patterns of labelling for connexin43 localized to calbindin-D28K-positive interstitial cells in the pineal gland. Eur J Neurosci. 2017;45:1553-1569 pubmed publisher
	Schimenti K, Feuer S, Griffin L, Graham N, Bovet C, Hartford S, et al. AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice. Genetics. 2013;194:447-57 pubmed publisher
	Kanady J, Dellinger M, Munger S, Witte M, Simon A. Connexin37 and Connexin43 deficiencies in mice disrupt lymphatic valve development and result in lymphatic disorders including lymphedema and chylothorax. Dev Biol. 2011;354:253-66 pubmed publisher
	Li X, Penes M, Odermatt B, Willecke K, Nagy J. Ablation of Cx47 in transgenic mice leads to the loss of MUPP1, ZONAB and multiple connexins at oligodendrocyte-astrocyte gap junctions. Eur J Neurosci. 2008;28:1503-17 pubmed publisher
	Rash J, Davidson K, Kamasawa N, Yasumura T, Kamasawa M, Zhang C, et al. Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb. J Neurocytol. 2005;34:307-41 pubmed
	Cluff A, Bystrom B, Klimaviciute A, Dahlqvist C, Cebers G, Malmstrom A, et al. Prolonged labour associated with lower expression of syndecan 3 and connexin 43 in human uterine tissue. Reprod Biol Endocrinol. 2006;4:24 pubmed
	Dobrenis K, Chang H, Pina Benabou M, Woodroffe A, Lee S, Rozental R, et al. Human and mouse microglia express connexin36, and functional gap junctions are formed between rodent microglia and neurons. J Neurosci Res. 2005;82:306-15 pubmed
	Kamasawa N, Sik A, Morita M, Yasumura T, Davidson K, Nagy J, et al. Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning. Neuroscience. 2005;136:65-86 pubmed
	Penes M, Li X, Nagy J. Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain. Eur J Neurosci. 2005;22:404-18 pubmed
	Li X, Ionescu A, Lynn B, Lu S, Kamasawa N, Morita M, et al. Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain. Neuroscience. 2004;126:611-30 pubmed
	Nagy J, Ionescu A, Lynn B, Rash J. Connexin29 and connexin32 at oligodendrocyte and astrocyte gap junctions and in myelin of the mouse central nervous system. J Comp Neurol. 2003;464:356-70 pubmed

product information

Product Type :

Antibody

Product Name :

Connexin 43 Monoclonal Antibody (3D8A5)

Catalog # :

35-5000

Quantity :

100 ug

Price :

US 451.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Mouse, Rat

Applications :

Immunohistochemistry (Frozen): Assay-dependent, Western Blot: Assay-dependent

Species :

Mouse, Rat

Clone :

3D8A5

Isotype :

IgG1

Storage :

-20 C

Description :

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

Immunogen :

Synthetic peptide derived from rat Connexin 43.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Frozen): Assay-dependent, Western Blot: Assay-dependent

Aliases :

alpha 1 connexin; AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin 43; connexin43; connexin-43; cx43; Cx-43; cx43 protein; cx43a1; Cx43alpha1; CXA1; Cxn-43; DFNB38; EKVP; etID309742.9; gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein; gap junction protein alpha 1; gap junction protein connexin 43; gap junction protein, alpha 1; gap junction protein, alpha 1, 43 kD (connexin 43); gap junction protein, alpha 1, 43kDa; gap junction protein, alpha 1, 43kDa (connexin 43); gja1; Gja-1; GJAL; HLHS1; HSS; Npm1; ODD; ODDD; ODOD; PPKCA; SDTY3; shf; Short fin protein; sof; stopsel; stp; Syndactyly type III; vascular smooth muscle connexin43; Vascular smooth muscle connexin-43; zfCx43.3

more info or order :

Invitrogen product webpage